A matched antibody pair for ELISA consists of two antibodies, a capture and a detection antibody. In a matched ELISA pair, each antibody is specific for a different and non-overlapping region or epitope of the antigen. By design, matched pairs function in a sandwich ELISA set-up; for further information on sandwich ELISA, guides and protocols go to the ELISA resource pages. Figure 1 below shows the sandwich ELISA protocol.

Fig. 1. Typical sandwich ELISA set-up using matched antibody pairs.

Fig. 1. Typical sandwich ELISA set-up using matched antibody pairs.

We have validated our matched antibody pairs in sandwich ELISA tests, removing any concerns about performance and reagent selection; the assay data can be found on the respective product pages. We also offer protein standards, as controls and for generation of a standard curve, for a subset of the matched ELISA pairs.

See the tables below for our matched ELISA antibody pairs for human, mouse, and rat.

Human

Table 1. Human matched ELISA antibody pairs.

Specificity

Antibody Type

Catalog Number

Description

Apolipoprotein A1

Capture

0650-0050

Mouse Anti-Human Apolipoprotein A1

Apolipoprotein A1

Detection

0650-0180

Sheep Anti-Human Apolipoprotein A1

Apolipoprotein A1

Detection

0650-0190

Sheep Anti-Human Apolipoprotein A1:HRP

BDNF

Capture

AHP1831

Rabbit Anti-Human BDNF

BDNF

Detection

AHP1831B

Rabbit Anti-Human BDNF:Biotin

BMP-2

Capture

AHP960

Rabbit Anti-Human BMP-2

BMP-2

Detection

AHP960B

Rabbit Anti-Human BMP-2:Biotin

CCL17

Capture

AHP1919

Rabbit Anti-Human CCL17

CCL17

Detection

AHP1919B

Rabbit Anti-Human CCL17:Biotin

CCL3L1

Capture

AHP1832

Goat Anti-Human CCL3L1

CCL3L1

Detection

AHP1832B

Goat Anti-Human CCL3L1:Biotin

CD120b

Capture

AHP1833

Rabbit Anti-Human CD120b

CD120b

Detection

AHP1833B

Rabbit Anti-Human CD120b:Biotin

CTGF

Capture

AHP1278

Rabbit Anti-Human CTGF

CTGF

Detection

AHP1278B

Rabbit Anti-Human CTGF:Biotin

CXCL13

Capture

AHP766

Rabbit Anti-Human CXCL13

CXCL13

Detection

AHP766B

Rabbit Anti-Human CXCL13:Biotin

Defensin beta 2

Capture

AHP849

Goat Anti-Human Defensin Beta 2

Defensin beta 2

Detection

AHP849B

Goat Anti-Human Defensin Beta 2:Biotin

EGF

Capture

AHP767

Rabbit Anti-Human EGF

EGF

Detection

AHP767B

Rabbit Anti-Human EGF:Biotin

FGF basic

Capture

AHP1038

Rabbit Anti-Human FGF Basic

FGF basic

Detection

AHP1038B

Rabbit Anti-Human FGF Basic:Biotin

FSH

Capture

0100-0663

Mouse Anti-Human FSH

FSH

Detection

0100-0662

Mouse Anti-Human FSH Alpha

FSH

Detection

0100-0662B

Mouse Anti-Human FSH Alpha:Biotin

G-CSF

Capture

1012701

Rat Anti-Human G-CSF

G-CSF

Detection

1012808

Rat Anti-Human G-CSF:Biotin

G-CSF

Capture

AHP1031

Rabbit Anti-Human G-CSF

G-CSF

Detection

AHP1031B

Rabbit Anti-Human G-CSF:Biotin

Grandzyme B

Capture

MCA2120

Mouse Anti-Grandzyme B

Grandzyme B

Detection

MCA2119B

Mouse Anti-Grandzyme B:Biotin

IFN gamma

Capture

AHP775

Rabbit Anti-Human Interferon Gamma

IFN gamma

Detection

AHP775B

Rabbit Anti-Human Interferon Gamma:Biotin

IFN gamma

Capture

HCA043

Human Anti-Human Interferon Gamma

IFN gamma

Capture

HCA043A

Human Anti-Human Interferon Gamma

IFN gamma

Detection

HCA044

Human Anti-Human Interferon Gamma

IFN gamma

Detection

HCA044A

Human Anti-Human Interferon Gamma

IFN gamma

Detection

HCA044P

Human Anti-Human Interferon Gamma:HRP

IGF-I

Capture

AHP1281

Rabbit Anti-Human Insulin-Like Growth Factor I

IGF-I

Detection

AHP1281B

Rabbit Anti-Human Insulin-Like Growth Factor I:Biotin

IL-1RA

Capture

AHP2184

Rabbit Anti-Human Interleukin-1RA

IL-1RA

Detection

AHP2184B

Rabbit Anti-Human Interleukin-1RA:Biotin

IL-2

Capture

AHP1032

Rabbit Anti-Human Interleukin-2

IL-2

Detection

AHP1032B

Rabbit Anti-Human Interleukin-2:Biotin

IL-2

Capture

MCA1552

Rat anti Human Interleukin-2

IL-2

Detection

AHP1032B

Rabbit Anti-Human Interleukin-2:Biotin

IL-5

Capture

AHP1034

Rabbit Anti-Human Interleukin-5

IL-5

Detection

AHP1034B

Rabbit Anti-Human Interleukin-5:Biotin

IL-6

Capture

1012001

Rat Anti-Human Interleukin-6

IL-6

Detection

1012108

Rat Anti-Human Interleukin-6:Biotin

IL-6

Capture

AHP1040

Rabbit Anti-Human Interleukin-6

IL-6

Detection

AHP1040B

Rabbit Anti-Human Interleukin-6:Biotin

IL-33

Capture

HCA140

Human Anti-Human Interleukin-33

IL-33

Capture

HCA140A

Human Anti-Human Interleukin-33

IL-33

Detection

AHP1482B

Rabbit Anti-Human Interleukin-33:Biotin

MCP-1

Capture

AHP784

Rabbit Anti-Human MCP-1

MCP-1

Capture

MCA2486

Mouse Anti-Human MCP-1

MCP-1

Detection

AHP784B

Rabbit Anti-Human MCP-1:Biotin

Prolactin

Capture

0100-0665

Mouse Anti-Human Prolactin

Prolactin

Detection

0100-0664

Mouse Anti-Human Prolactin

RANTES

Capture

AHP340G

Rabbit Anti-Human RANTES

RANTES

Detection

AHP340B

Rabbit Anti-Human RANTES:Biotin

SDF-1 alpha

Capture

AHP794

Rabbit Anti-Human SDF-1 Alpha

SDF-1 alpha

Detection

AHP794B

Rabbit Anti-Human SDF-1 Alpha:Biotin

Thyroglobulin

Capture

8900-0700

Mouse Anti-Human Thyroglobulin

Thyroglobulin

Detection

8900-0730

Mouse Anti-Human Thyroglobulin

TNF alpha

Capture

AHP1212

Goat Anti-Human TNF Alpha

TNF alpha

Detection

AHP1212B

Goat Anti-Human TNF Alpha:Biotin

VEGF

Capture

AHP831

Goat Anti-Human VEGF

VEGF

Detection

AHP831B

Goat Anti-Human VEGF:Biotin

WNT-3a

Capture

AHP2188

Rabbit Anti-Human WNT-3a

WNT-3a

Detection

AHP2188B

Rabbit Anti-Human WNT-3a:Biotin

Mouse

Table 2. Mouse matched ELISA antibody pairs.

Specificity

Antibody Type

Catalog Number

Description

CXCL1

Capture

AAM40

Rabbit Anti-Mouse CXCL1

CXCL1

Detection

AAM40B

Rabbit Anti-Mouse CXCL1:Biotin

IgG1

Capture

MCA1289

Rat Anti-Mouse IgG1

IgG1

Detection

MCA336P

Rat Anti-Mouse IgG1 Heavy Chain:HRP

IgG2a

Capture

MCA1588

Rat Anti-Mouse IgG2a Heavy Chain

IgG2a

Detection

MCA421P

Rat Anti-Mouse IgG2a Heavy Chain:HRP

IgG2b

Capture

MCA1589

Rat Anti-Mouse IgG2b

IgG2b

Detection

MCA1291P

Rat Anti-Mouse IgG2b:HRP

IgG3

Capture

MCA1292

Rat Anti-Mouse IgG3

IgG3

Detection

MCA423B

Rat Anti-Mouse IgG3 Heavy Chain:Biotin

IgM

Capture

MCA1293

Rat Anti-Mouse IgM Heavy Chain

IgM

Detection

MCA199B

Rat Anti-Mouse IgM Heavy Chain:Biotin

IL-1 beta

Capture

AAM13G

Rabbit Anti-Mouse Interleukin-1 Beta

IL-1 beta

Detection

AAM13B

Rabbit Anti-Mouse Interleukin-1 Beta:Biotin

IL-2

Capture

AAM35

Rabbit Anti-Mouse Interleukin-2

IL-2

Detection

AAM35B

Rabbit Anti-Mouse Interleukin-2:Biotin

IL-13

Capture

AAM34

Rabbit Anti-Mouse Interleukin-13

IL-13

Detection

AAM34B

Rabbit Anti-Mouse Interleukin-13:Biotin

IL-21

Capture

AAM61

Rabbit Anti-Mouse Interleukin-21

IL-21

Detection

AAM61B

Rabbit Anti-Mouse Interleukin-21:Biotin

IP-10

Capture

AAM39

Rabbit Anti-Mouse IP-10

IP-10

Detection

AAM39B

Rabbit Anti-Mouse IP-10:Biotin

MIP-2

Capture

AAM48

Rabbit Anti-Mouse MIP-2

MIP-2

Detection

AAM48B

Rabbit Anti-Mouse MIP-2:Biotin

SDF-1 alpha

Capture

AAM64

Goat Anti-Mouse SDF-1 Alpha

SDF-1 alpha

Detection

AAM64B

Goat Anti-Mouse SDF-1 Alpha:Biotin

VEGF

Capture

AAM51

Rabbit Anti-Mouse VEGF

VEGF

Detection

AAM51B

Rabbit Anti-Mouse VEGF:Biotin

Rat

Table 3. Rat matched ELISA antibody pairs.

Specificity

Antibody

Catalog Number

Description

IgE

Capture

MCA191

Mouse Anti-Rat IgA Heavy Chain

IgG1

Capture

MCA190

Mouse Anti-Rat IgD

IgE

Capture

MCA193

Mouse Anti-Rat IgE

IgG1

Capture

MCA194

Mouse Anti-Rat IgG1 Heavy Chain

IgG2a

Capture

MCA278

Mouse Anti-Rat IgG2a Heavy Chain

IgG2b

Capture

MCA1294

Mouse Anti-Rat IgG2b Heavy Chain

IgG2c

Capture

MCA1295

Mouse Anti-Rat IgG2c Heavy Chain

IgM

Capture

MCA189

Mouse Anti-Rat IgM Heavy Chain

Kappa/Lambda Light Chain

Detection

MCA1296P

Mouse Anti-Rat Kappa/Lambda Light Chain:HRP

IL-1 beta

Capture

AAR15G

Rabbit Anti-Rat Interleukin-1 Beta

IL-1 beta

Detection

AAR15B

Rabbit Anti-Rat Interleukin-1 Beta:Biotin

RANTES

Capture

AAR18G

Rabbit Anti-Rat RANTES

RANTES

Detection

AAR18B

Rabbit Anti-Rat RANTES:Biotin

VEGF

Capture

AAR42

Rabbit Anti-Rat VEGF

VEGF

Detection

AAR42B

Rabbit Anti-Rat VEGF:Biotin