Matched Antibody Pairs for ELISA
s
Overview
A matched antibody pair for ELISA consists of a capture antibody and a detection antibody. In a matched ELISA pair, each antibody is specific for a different and non-overlapping region or epitope of the antigen. By design, matched pairs function in a sandwich ELISA set-up; for further information on sandwich ELISA, guides and protocols go to the main ELISA page. Figure 1 below shows the sandwich ELISA protocol.
Fig. 1. Typical sandwich ELISA set-up using matched antibody pairs.
We have validated our matched antibody pairs in sandwich ELISA tests, removing any concerns about performance and reagent selection; the assay data can be found on the respective product pages. We also offer protein standards, as controls and for generation of a standard curve, for a subset of the matched ELISA pairs.
View our recommended ELISA protocol for use with matched antibody pairs.
See the tables below for our range of matched ELISA antibody pairs for the following species:
Human
Table 1. Human matched ELISA antibody pairs.
Specificity |
Antibody Type |
Catalog Number |
Description |
|---|---|---|---|
|
BDNF |
Capture |
Rabbit Anti-Human BDNF |
|
|
BDNF |
Detection |
Rabbit Anti-Human BDNF:Biotin |
|
|
BMP-2 |
Capture |
Rabbit Anti-Human BMP-2 |
|
|
BMP-2 |
Detection |
Rabbit Anti-Human BMP-2:Biotin |
|
|
CCL2 |
Capture |
Mouse Anti-Human CCL2 |
|
|
CCL2 |
Detection |
Mouse Anti-Human CCL2:Biotin |
|
|
CCL3 |
Capture |
Mouse Anti-Human CCL3 |
|
|
CCL3 |
Detection |
Mouse Anti-Human CCL3:Biotin |
|
|
CCL17 |
Capture |
Rabbit Anti-Human CCL17 |
|
|
CCL17 |
Detection |
Rabbit Anti-Human CCL17:Biotin |
|
|
CCL17 |
Capture |
Mouse Anti-Human CCL17 |
|
|
CCL17 |
Detection |
Mouse Anti-Human CCL17:Biotin |
|
|
CCL24 |
Capture |
Mouse Anti-Human CCL24 |
|
|
CCL24 |
Detection |
Mouse Anti-Human CCL24:Biotin |
|
|
CD120b |
Capture |
Rabbit Anti-Human CD120b |
|
|
CD120b |
Detection |
Rabbit Anti-Human CD120b:Biotin |
|
|
CRP |
Capture |
Mouse Anti-Human CRP |
|
|
CRP |
Detection |
Mouse Anti-Human CRP:Biotin |
|
|
CTGF |
Capture |
Rabbit Anti-Human CTGF |
|
|
CTGF |
Detection |
Rabbit Anti-Human CTGF:Biotin |
|
|
CXCL10 |
Capture |
Mouse Anti-Human CXCL10 |
|
|
CXCL10 |
Detection |
Mouse Anti-Human CXCL10:Biotin |
|
|
CXCL13 |
Capture |
Rabbit Anti-Human CXCL13 |
|
|
CXCL13 |
Detection |
Rabbit Anti-Human CXCL13:Biotin |
|
|
Cystatin C |
Capture |
Mouse Anti-Human Cystatin C |
|
|
Cystatin C |
Detection |
Mouse Anti-Human Cystatin C:Biotin |
|
|
Defensin beta 2 |
Capture |
Goat Anti-Human Defensin Beta 2 |
|
|
Defensin beta 2 |
Detection |
Goat Anti-Human Defensin Beta 2:Biotin |
|
|
EGF |
Capture |
Rabbit Anti-Human EGF |
|
|
EGF |
Detection |
Rabbit Anti-Human EGF:Biotin |
|
|
FGF basic |
Capture |
Rabbit Anti-Human FGF Basic |
|
|
FGF basic |
Detection |
Rabbit Anti-Human FGF Basic:Biotin |
|
|
Factor H |
Capture |
Mouse Anti-Human Complement Factor H |
|
|
Factor H |
Detection |
Mouse Anti-Human Complement Factor H:Biotin |
|
|
Fibrinogen |
Capture |
Mouse Anti-Human Fibrinogen |
|
|
Fibrinogen |
Detection |
Mouse Anti-Human Fibrinogen:Biotin |
|
|
G-CSF |
Capture |
Rat Anti-Human G-CSF |
|
|
G-CSF |
Detection |
Rat Anti-Human G-CSF:Biotin |
|
|
G-CSF |
Capture |
Rabbit Anti-Human G-CSF |
|
|
G-CSF |
Detection |
Rabbit Anti-Human G-CSF:Biotin |
|
|
Granzyme B |
Capture |
Mouse Anti-Granzyme B |
|
|
Granzyme B |
Detection |
Mouse Anti-Granzyme B:Biotin |
|
|
Haptoglobin |
Capture |
Mouse Anti-Human Haptoglobin |
|
|
Haptoglobin |
Detection |
Mouse Anti-Human Haptoglobin:Biotin |
|
|
IFN Beta |
Capture |
Mouse Anti-Human IFN Beta |
|
|
IFN Beta |
Detection |
Mouse Anti-Human IFN Beta:Biotin |
|
|
IFN gamma |
Capture |
Mouse Anti-Human IFN Gamma |
|
|
IFN gamma |
Detection |
Mouse Anti-Human IFN Gamma:Biotin |
|
|
IFN gamma |
Capture |
Rabbit Anti-Human Interferon Gamma |
|
|
IFN gamma |
Detection |
Rabbit Anti-Human Interferon Gamma:Biotin |
|
|
IFN gamma |
Capture |
Human Anti-Human Interferon Gamma |
|
|
IFN gamma |
Detection |
Human Anti-Human Interferon Gamma |
|
|
IGF-I |
Capture |
Rabbit Anti-Human Insulin-Like Growth Factor I |
|
|
IGF-I |
Detection |
Rabbit Anti-Human Insulin-Like Growth Factor I:Biotin |
|
|
IL-2 |
Capture |
Rabbit Anti-Human Interleukin-2 |
|
|
IL-2 |
Detection |
Rabbit Anti-Human Interleukin-2:Biotin |
|
|
IL-2 |
Capture |
Rat anti Human Interleukin-2 |
|
|
IL-2 |
Detection |
Rabbit Anti-Human Interleukin-2:Biotin |
|
|
IL-5 |
Capture |
Rabbit Anti-Human Interleukin-5 |
|
|
IL-5 |
Detection |
Rabbit Anti-Human Interleukin-5:Biotin |
|
|
IL-6 |
Capture |
Mouse Anti-Human Interleukin-6 |
|
|
IL-6 |
Detection |
Mouse Anti-Human Interleukin-6:Biotin |
|
|
IL-6 |
Capture |
Rabbit Anti-Human Interleukin-6 |
|
|
IL-6 |
Detection |
Rabbit Anti-Human Interleukin-6:Biotin |
|
|
IL-11 |
Capture |
Mouse Anti-Human Interleukin-11 |
|
|
IL-11 |
Detection |
Mouse Anti-Human Interleukin-11:Biotin |
|
|
IL-12 p40 |
Capture |
Mouse Anti-Human Interleukin-12p40 |
|
|
IL-12 p40 |
Detection |
Mouse Anti-Human Interleukin-12p40:Biotin |
|
|
MMP-2 |
Capture |
Mouse Anti-Human MMP-2 |
|
|
MMP-2 |
Detection |
Mouse Anti-Human MMP-2:Biotin |
|
|
MMP-9 |
Capture |
Mouse Anti-Human MMP-9 |
|
|
MMP-9 |
Detection |
Mouse Anti-Human MMP-9:Biotin |
|
|
MPO |
Capture |
Mouse Anti-Human Myeloperoxidase |
|
|
MPO |
Detection |
Mouse Anti-Human Myeloperoxidase:Biotin |
|
|
PDGF-BB |
Capture |
Mouse Anti-Human PDGF-BB |
|
|
PDGF-BB |
Detection |
Mouse Anti-Human PDGF-BB:Biotin |
|
|
Prolactin |
Capture |
Mouse Anti-Human Prolactin |
|
|
Prolactin |
Detection |
Mouse Anti-Human Prolactin |
|
|
RANTES |
Capture |
Rabbit Anti-Human RANTES |
|
|
RANTES |
Detection |
Rabbit Anti-Human RANTES:Biotin |
|
|
SAP |
Capture |
Mouse Anti-Human SAP |
|
|
SAP |
Detection |
Mouse Anti-Human SAP:Biotin |
|
|
SDF-1 alpha |
Capture |
Rabbit Anti-Human SDF-1 Alpha |
|
|
SDF-1 alpha |
Detection |
Rabbit Anti-Human SDF-1 Alpha:Biotin |
|
|
TNF alpha |
Capture |
Goat Anti-Human TNF Alpha |
|
|
TNF alpha |
Detection |
Goat Anti-Human TNF Alpha:Biotin |
|
|
VEGF |
Capture |
Mouse Anti-Human VEGF |
|
|
VEGF |
Detection |
Mouse Anti-Human VEGF:Biotin |
|
|
VEGF |
Capture |
Goat Anti-Human VEGF |
|
|
VEGF |
Detection |
Goat Anti-Human VEGF:Biotin |
|
|
vWF |
Capture |
Mouse Anti-Human vWF |
|
|
vWF |
Detection |
Mouse Anti-Human vWF:Biotin |
|
|
WNT-3a |
Capture |
Rabbit Anti-Human WNT-3a |
|
|
WNT-3a |
Detection |
Rabbit Anti-Human WNT-3a:Biotin |
Mouse
Table 2. Mouse matched ELISA antibody pairs.
Specificity |
Antibody Type |
Catalog Number |
Description |
|---|---|---|---|
|
IgG1 |
Capture |
Rat Anti-Mouse IgG1 |
|
|
IgG1 |
Detection |
Rat Anti-Mouse IgG1 Heavy Chain:HRP |
|
|
IgG2a |
Capture |
Rat Anti-Mouse IgG2a Heavy Chain |
|
|
IgG2a |
Detection |
Rat Anti-Mouse IgG2a Heavy Chain:HRP |
|
|
IgG2b |
Capture |
Rat Anti-Mouse IgG2b |
|
|
IgG2b |
Detection |
Rat Anti-Mouse IgG2b:HRP |
|
|
IgM |
Capture |
Rat Anti-Mouse IgM Heavy Chain |
|
|
IgM |
Detection |
Rat Anti-Mouse IgM Heavy Chain:Biotin |
|
|
IL-1 beta |
Capture |
Rabbit Anti-Mouse Interleukin-1 Beta |
|
|
IL-1 beta |
Detection |
Rabbit Anti-Mouse Interleukin-1 Beta:Biotin |
|
|
IL-13 |
Capture |
Rabbit Anti-Mouse Interleukin-13 |
|
|
IL-13 |
Detection |
Rabbit Anti-Mouse Interleukin-13:Biotin |
|
|
IL-21 |
Capture |
Rabbit Anti-Mouse Interleukin-21 |
|
|
IL-21 |
Detection |
Rabbit Anti-Mouse Interleukin-21:Biotin |
|
|
IP-10 |
Capture |
Rabbit Anti-Mouse IP-10 |
|
|
IP-10 |
Detection |
Rabbit Anti-Mouse IP-10:Biotin |
|
|
MIP-2 |
Capture |
Rabbit Anti-Mouse MIP-2 |
|
|
MIP-2 |
Detection |
Rabbit Anti-Mouse MIP-2:Biotin |
|
|
SDF-1 alpha |
Capture |
Goat Anti-Mouse SDF-1 Alpha |
|
|
SDF-1 alpha |
Detection |
Goat Anti-Mouse SDF-1 Alpha:Biotin |
|
|
VEGF |
Capture |
Rabbit Anti-Mouse VEGF |
|
|
VEGF |
Detection |
Rabbit Anti-Mouse VEGF:Biotin |
Rat
Table 3. Rat matched ELISA antibody pairs.
Specificity |
Antibody |
Catalog Number |
Description |
|---|---|---|---|
|
IL-1 beta |
Capture |
Rabbit Anti-Rat Interleukin-1 Beta |
|
|
IL-1 beta |
Detection |
Rabbit Anti-Rat Interleukin-1 Beta:Biotin |
|
|
VEGF |
Capture |
Rabbit Anti-Rat VEGF |
|
|
VEGF |
Detection |
Rabbit Anti-Rat VEGF:Biotin |
Bovine
Table 4. Bovine cytokine antibody pairs for ELISA.
| Specificity & Format | Format | Clone |
|---|---|---|
| GM-CSF (capture) | Purified | Polyclonal |
| GM-CSF (detection) | Biotin | Polyclonal |
| IFN gamma (capture) | Purified | CC330 |
| IFN gamma (detection) | Biotin | CC302 |
| IL-4 (capture) | Purified | CC313 |
| IL-4 (detection) | Biotin | CC314 |
| IL-6 (capture) | Purified | Polyclonal |
| IL-6 (detection) | Biotin | Polyclonal |
| IL-8 (capture) | Purified | Polyclonal |
| IL-8 (detection) | Biotin | Polyclonal |
| IL-10 (capture) | Purified | CC318 |
| IL-10 (detection) | Biotin | CC320 |
| IL-17A (capture) | Purified | Polyclonal |
| IL-17A (detection) | Biotin | Polyclonal |
| IL-22 (capture) | Purified | Monoclonal |
| IL-22 (detection) | Purified | Monoclonal |
| TNF-alpha (capture) | Purified | CC327 |
| TNF-alpha (detection) | Biotin | CC328 |
| TNF alpha (capture) | Purified | Polyclonal |
| TNF alpha (detection) | Biotin | Polyclonal |