Matched Antibody Pairs for ELISA
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Introduction to ELISA Basics Guide
The ELISA Basics Guide has the right amount of detail to help you plan your experiment and achieve a successful ELISA.
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Overview
A matched antibody pair for ELISA consists of two antibodies, a capture and a detection antibody. In a matched ELISA pair, each antibody is specific for a different and non-overlapping region or epitope of the antigen. By design, matched pairs function in a sandwich ELISA set-up; for further information on sandwich ELISA, guides and protocols go to the ELISA resource pages. Figure 1 below shows the sandwich ELISA protocol.
Fig. 1. Typical sandwich ELISA set-up using matched antibody pairs.
We have validated our matched antibody pairs in sandwich ELISA tests, removing any concerns about performance and reagent selection; the assay data can be found on the respective product pages. We also offer protein standards, as controls and for generation of a standard curve, for a subset of the matched ELISA pairs.
See the tables below for our matched ELISA antibody pairs for human, mouse, and rat.
Human
Table 1. Human matched ELISA antibody pairs.
Specificity |
Antibody Type |
Catalog Number |
Description |
|---|---|---|---|
|
Apolipoprotein A1 |
Capture |
Mouse Anti-Human Apolipoprotein A1 |
|
|
Apolipoprotein A1 |
Detection |
Sheep Anti-Human Apolipoprotein A1 |
|
|
Apolipoprotein A1 |
Detection |
Sheep Anti-Human Apolipoprotein A1:HRP |
|
|
BDNF |
Capture |
Rabbit Anti-Human BDNF |
|
|
BDNF |
Detection |
Rabbit Anti-Human BDNF:Biotin |
|
|
BMP-2 |
Capture |
Rabbit Anti-Human BMP-2 |
|
|
BMP-2 |
Detection |
Rabbit Anti-Human BMP-2:Biotin |
|
|
CCL17 |
Capture |
Rabbit Anti-Human CCL17 |
|
|
CCL17 |
Detection |
Rabbit Anti-Human CCL17:Biotin |
|
|
CCL3L1 |
Capture |
Goat Anti-Human CCL3L1 |
|
|
CCL3L1 |
Detection |
Goat Anti-Human CCL3L1:Biotin |
|
|
CD120b |
Capture |
Rabbit Anti-Human CD120b |
|
|
CD120b |
Detection |
Rabbit Anti-Human CD120b:Biotin |
|
|
CTGF |
Capture |
Rabbit Anti-Human CTGF |
|
|
CTGF |
Detection |
Rabbit Anti-Human CTGF:Biotin |
|
|
CXCL13 |
Capture |
Rabbit Anti-Human CXCL13 |
|
|
CXCL13 |
Detection |
Rabbit Anti-Human CXCL13:Biotin |
|
|
Defensin beta 2 |
Capture |
Goat Anti-Human Defensin Beta 2 |
|
|
Defensin beta 2 |
Detection |
Goat Anti-Human Defensin Beta 2:Biotin |
|
|
EGF |
Capture |
Rabbit Anti-Human EGF |
|
|
EGF |
Detection |
Rabbit Anti-Human EGF:Biotin |
|
|
FGF basic |
Capture |
Rabbit Anti-Human FGF Basic |
|
|
FGF basic |
Detection |
Rabbit Anti-Human FGF Basic:Biotin |
|
|
FSH |
Capture |
Mouse Anti-Human FSH |
|
|
FSH |
Detection |
Mouse Anti-Human FSH Alpha |
|
|
FSH |
Detection |
Mouse Anti-Human FSH Alpha:Biotin |
|
|
G-CSF |
Capture |
Rat Anti-Human G-CSF |
|
|
G-CSF |
Detection |
Rat Anti-Human G-CSF:Biotin |
|
|
G-CSF |
Capture |
Rabbit Anti-Human G-CSF |
|
|
G-CSF |
Detection |
Rabbit Anti-Human G-CSF:Biotin |
|
|
Grandzyme B |
Capture |
Mouse Anti-Grandzyme B |
|
|
Grandzyme B |
Detection |
Mouse Anti-Grandzyme B:Biotin |
|
|
IFN gamma |
Capture |
Rabbit Anti-Human Interferon Gamma |
|
|
IFN gamma |
Detection |
Rabbit Anti-Human Interferon Gamma:Biotin |
|
|
IFN gamma |
Capture |
Human Anti-Human Interferon Gamma |
|
|
IFN gamma |
Capture |
Human Anti-Human Interferon Gamma |
|
|
IFN gamma |
Detection |
Human Anti-Human Interferon Gamma |
|
|
IFN gamma |
Detection |
Human Anti-Human Interferon Gamma |
|
|
IFN gamma |
Detection |
Human Anti-Human Interferon Gamma:HRP |
|
|
IGF-I |
Capture |
Rabbit Anti-Human Insulin-Like Growth Factor I |
|
|
IGF-I |
Detection |
Rabbit Anti-Human Insulin-Like Growth Factor I:Biotin |
|
|
IL-1RA |
Capture |
Rabbit Anti-Human Interleukin-1RA |
|
|
IL-1RA |
Detection |
Rabbit Anti-Human Interleukin-1RA:Biotin |
|
|
IL-2 |
Capture |
Rabbit Anti-Human Interleukin-2 |
|
|
IL-2 |
Detection |
Rabbit Anti-Human Interleukin-2:Biotin |
|
|
IL-2 |
Capture |
Rat anti Human Interleukin-2 |
|
|
IL-2 |
Detection |
Rabbit Anti-Human Interleukin-2:Biotin |
|
|
IL-5 |
Capture |
Rabbit Anti-Human Interleukin-5 |
|
|
IL-5 |
Detection |
Rabbit Anti-Human Interleukin-5:Biotin |
|
|
IL-6 |
Capture |
Rat Anti-Human Interleukin-6 |
|
|
IL-6 |
Detection |
Rat Anti-Human Interleukin-6:Biotin |
|
|
IL-6 |
Capture |
Rabbit Anti-Human Interleukin-6 |
|
|
IL-6 |
Detection |
Rabbit Anti-Human Interleukin-6:Biotin |
|
|
MCP-1 |
Capture |
Rabbit Anti-Human MCP-1 |
|
|
MCP-1 |
Capture |
Mouse Anti-Human MCP-1 |
|
|
MCP-1 |
Detection |
Rabbit Anti-Human MCP-1:Biotin |
|
|
Prolactin |
Capture |
Mouse Anti-Human Prolactin |
|
|
Prolactin |
Detection |
Mouse Anti-Human Prolactin |
|
|
RANTES |
Capture |
Rabbit Anti-Human RANTES |
|
|
RANTES |
Detection |
Rabbit Anti-Human RANTES:Biotin |
|
|
SDF-1 alpha |
Capture |
Rabbit Anti-Human SDF-1 Alpha |
|
|
SDF-1 alpha |
Detection |
Rabbit Anti-Human SDF-1 Alpha:Biotin |
|
|
Thyroglobulin |
Capture |
Mouse Anti-Human Thyroglobulin |
|
|
Thyroglobulin |
Detection |
Mouse Anti-Human Thyroglobulin |
|
|
TNF alpha |
Capture |
Goat Anti-Human TNF Alpha |
|
|
TNF alpha |
Detection |
Goat Anti-Human TNF Alpha:Biotin |
|
|
VEGF |
Capture |
Goat Anti-Human VEGF |
|
|
VEGF |
Detection |
Goat Anti-Human VEGF:Biotin |
|
|
WNT-3a |
Capture |
Rabbit Anti-Human WNT-3a |
|
|
WNT-3a |
Detection |
Rabbit Anti-Human WNT-3a:Biotin |
Mouse
Table 2. Mouse matched ELISA antibody pairs.
Specificity |
Antibody Type |
Catalog Number |
Description |
|---|---|---|---|
|
CXCL1 |
Capture |
Rabbit Anti-Mouse CXCL1 |
|
|
CXCL1 |
Detection |
Rabbit Anti-Mouse CXCL1:Biotin |
|
|
IgG1 |
Capture |
Rat Anti-Mouse IgG1 |
|
|
IgG1 |
Detection |
Rat Anti-Mouse IgG1 Heavy Chain:HRP |
|
|
IgG2a |
Capture |
Rat Anti-Mouse IgG2a Heavy Chain |
|
|
IgG2a |
Detection |
Rat Anti-Mouse IgG2a Heavy Chain:HRP |
|
|
IgG2b |
Capture |
Rat Anti-Mouse IgG2b |
|
|
IgG2b |
Detection |
Rat Anti-Mouse IgG2b:HRP |
|
|
IgG3 |
Capture |
Rat Anti-Mouse IgG3 |
|
|
IgG3 |
Detection |
Rat Anti-Mouse IgG3 Heavy Chain:Biotin |
|
|
IgM |
Capture |
Rat Anti-Mouse IgM Heavy Chain |
|
|
IgM |
Detection |
Rat Anti-Mouse IgM Heavy Chain:Biotin |
|
|
IL-1 beta |
Capture |
Rabbit Anti-Mouse Interleukin-1 Beta |
|
|
IL-1 beta |
Detection |
Rabbit Anti-Mouse Interleukin-1 Beta:Biotin |
|
|
IL-2 |
Capture |
Rabbit Anti-Mouse Interleukin-2 |
|
|
IL-2 |
Detection |
Rabbit Anti-Mouse Interleukin-2:Biotin |
|
|
IL-13 |
Capture |
Rabbit Anti-Mouse Interleukin-13 |
|
|
IL-13 |
Detection |
Rabbit Anti-Mouse Interleukin-13:Biotin |
|
|
IL-21 |
Capture |
Rabbit Anti-Mouse Interleukin-21 |
|
|
IL-21 |
Detection |
Rabbit Anti-Mouse Interleukin-21:Biotin |
|
|
IP-10 |
Capture |
Rabbit Anti-Mouse IP-10 |
|
|
IP-10 |
Detection |
Rabbit Anti-Mouse IP-10:Biotin |
|
|
MIP-2 |
Capture |
Rabbit Anti-Mouse MIP-2 |
|
|
MIP-2 |
Detection |
Rabbit Anti-Mouse MIP-2:Biotin |
|
|
SDF-1 alpha |
Capture |
Goat Anti-Mouse SDF-1 Alpha |
|
|
SDF-1 alpha |
Detection |
Goat Anti-Mouse SDF-1 Alpha:Biotin |
|
|
VEGF |
Capture |
Rabbit Anti-Mouse VEGF |
|
|
VEGF |
Detection |
Rabbit Anti-Mouse VEGF:Biotin |
Rat
Table 3. Rat matched ELISA antibody pairs.
Specificity |
Antibody |
Catalog Number |
Description |
|---|---|---|---|
|
IgE |
Capture |
Mouse Anti-Rat IgA Heavy Chain |
|
|
IgG1 |
Capture |
Mouse Anti-Rat IgD |
|
|
IgE |
Capture |
Mouse Anti-Rat IgE |
|
|
IgG1 |
Capture |
Mouse Anti-Rat IgG1 Heavy Chain |
|
|
IgG2a |
Capture |
Mouse Anti-Rat IgG2a Heavy Chain |
|
|
IgG2b |
Capture |
Mouse Anti-Rat IgG2b Heavy Chain |
|
|
IgG2c |
Capture |
Mouse Anti-Rat IgG2c Heavy Chain |
|
|
IgM |
Capture |
Mouse Anti-Rat IgM Heavy Chain |
|
|
Kappa/Lambda Light Chain |
Detection |
Mouse Anti-Rat Kappa/Lambda Light Chain:HRP |
|
|
IL-1 beta |
Capture |
Rabbit Anti-Rat Interleukin-1 Beta |
|
|
IL-1 beta |
Detection |
Rabbit Anti-Rat Interleukin-1 Beta:Biotin |
|
|
RANTES |
Capture |
Rabbit Anti-Rat RANTES |
|
|
RANTES |
Detection |
Rabbit Anti-Rat RANTES:Biotin |
|
|
VEGF |
Capture |
Rabbit Anti-Rat VEGF |
|
|
VEGF |
Detection |
Rabbit Anti-Rat VEGF:Biotin |