Mouse anti Bovine Interleukin-10 antibody, clone CC318 recognizes bovine IL-10. Mouse anti Bovine Interleukin-10 antibody, clone CC318 has been shown not to inhibit the biological activity of IL-10.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Plasmid cDNA encoding bovine IL-10.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
response to molecule of bacterial origin
negative regulation of cytokine secretion involved in immune response
positive regulation of B cell apoptosis
negative regulation of B cell proliferation
negative regulation of interleukin-6 production
receptor biosynthetic process
interferon-gamma biosynthetic process
positive regulation of gene-specific transcription
negative regulation of nitric oxide biosynthetic process
negative regulation of interferon-gamma biosynthetic process
positive regulation of cytokine secretion
negative regulation of membrane protein ectodomain proteolysis
positive regulation of transcription factor activity
response to glucocorticoid stimulus
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IL-10 antibody
|Flow Cytometry 1
- 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- This reagent may be used as a capture antibody in a sandwich ELISA assay for bovine IL-10 in combination with MCA2111B as detection reagent, see Bannerman, D.D.et al.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for IL-10 antibody
Kwong, L.S. et al. (2002) Development of an ELISA for bovine IL-10.
Vet Immunol Immunopathol. 85 (3-4): 213-23.
Scandurra, G.M. et al. (2009) Assessment of live candidate vaccines for paratuberculosis in animal models and macrophages.
Infect Immun. 78: 1383-9.
Weiss DJ et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
J Leukoc Biol. 83 (1): 48-55.
Hamza, E. et al. (2007) Modulation of allergy incidence in icelandic horses is associated with a change in IL-4-producing T cells.
Int Arch Allergy Immunol. 144: 325-37.
Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
J Dairy Sci. 93: 2458-70.
Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
Funct Integr Genomics. 10: 21-38.
Parker, D.G. et al. (2010) Lentivirus-mediated gene transfer of interleukin 10 to the ovine and human cornea.
Clin Experiment Ophthalmol. 38: 405-13.
Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
Vet Res. 42:19.
Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
Clin Diagn Lab Immunol. 11: 463-72.
Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.
Vet Res. 41: 14.
den Hartog, G. et al. (2011) Modulation of human immune responses by bovine interleukin-10.
PLoSone 6: e18188
Ikebuchi, R. et al. (2013) Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro.
Vet Res. 44: 59.
Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
PLoS One. 5: e13705.
Jones, G.J. et al. (2010) Simultaneous measurement of antigen-stimulated interleukin-1 beta and gamma interferon production enhances test sensitivity for the detection of Mycobacterium bovis infection in cattle.
Clin Vaccine Immunol. 17: 1946-51.
McGill, J.L. et al. (2013) Differential chemokine and cytokine production by neonatal bovine γ/δ T-cell subsets in response to viral toll-like receptor agonists and in vivo respiratory syncytial virus infection.
Immunology. 139: 227-44.
Olivier, M. et al. (2009) Capacities of migrating CD1b+ lymph dendritic cells to present Salmonella antigens to naive T cells.
PLoS One. 7: e30430.
Shu, D. et al. (2011) Diverse cytokine profile from mesenteric lymph node cells of cull cows severely affected with Johne's disease.
Clin Vaccine Immunol. 18: 1467-76.
Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
J Comp Pathol. 150 (4): 434-48.
Dooley LM et al. (2015) Effect of mesenchymal precursor cells on the systemic inflammatory response and endothelial dysfunction in an ovine model of collagen-induced arthritis.
PLoS One. 10 (5): e0124144.
Rainard, P. et al. (2016) Innate and Adaptive Immunity Synergize to Trigger Inflammation in the Mammary Gland.
PLoS One. 11 (4): e0154172.
Canal AM et al. (2017) Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection.
Res Vet Sci. 110: 34-39.
Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.
Infect Immun. 85 (2)Jan 26 [Epub ahead of print].
Pomeroy B et al. (2016) Impact of in vitro treatments of physiological levels of estradiol and progesterone observed in pregnancy on bovine monocyte-derived dendritic cell differentiation and maturation.
Vet Immunol Immunopathol. 182: 37-42.