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IL-10 antibody | CC318

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Anti Bovine Interleukin-10 Antibody, clone CC318 gallery image 1
Published customer image:
Mouse anti Bovine interleukin-10 antibody, clone CC318 (MCA2110) used as a capture reagent for the determination of IL-10 levels in ovine plasma by ELISA
Image caption:
Comparison of plasma concentrations of (A) IL-10 and (B) IL-6 measured over time in arthritic sheep treated and untreated with mesenchymal precursor cells (MPC). Each point represents the mean ± SEM of 7–8 sheep.**** Indicates a statistically significant difference between groups at that time point, p ≤ 0.0001.

From: Dooley LM, Abdalmula A, Washington EA, Kaufman C, Tudor EM, Ghosh P, et al. (2015)
Effect of Mesenchymal Precursor Cells on the Systemic Inflammatory Response and Endothelial Dysfunction in an Ovine Model of Collagen-Induced Arthritis.
PLoS ONE 10(5): e0124144.

Mouse anti Bovine Interleukin-10

Product Type
Monoclonal Antibody
Clone
CC318
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2110 E ES F* 0.5 mg
Mouse anti Bovine Interleukin-10 antibody, clone CC318 recognizes bovine IL-10. Mouse anti Bovine Interleukin-10 antibody, clone CC318 has been shown not to inhibit the biological activity of IL-10.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Horse
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Plasmid cDNA encoding bovine IL-10.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P43480 Related reagents
Entrez Gene
IL10 Related reagents
GO Terms
GO:0002237 response to molecule of bacterial origin
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0002904 positive regulation of B cell apoptosis
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0030889 negative regulation of B cell proliferation
GO:0032715 negative regulation of interleukin-6 production
GO:0032800 receptor biosynthetic process
GO:0042095 interferon-gamma biosynthetic process
GO:0043193 positive regulation of gene-specific transcription
GO:0045019 negative regulation of nitric oxide biosynthetic process
GO:0045077 negative regulation of interferon-gamma biosynthetic process
GO:0050715 positive regulation of cytokine secretion
GO:0051045 negative regulation of membrane protein ectodomain proteolysis
GO:0051091 positive regulation of transcription factor activity
GO:0051384 response to glucocorticoid stimulus
Regulatory
For research purposes only

Applications of IL-10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml 10ug/ml
ELISpot
Flow Cytometry 1
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This reagent may be used as a capture antibody in a sandwich ELISA assay for bovine IL-10 in combination with MCA2111B as detection reagent, see Bannerman, D.D.et al.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F 0.1 mg F
Human anti Mouse IgG2b:HRP HCA038P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2b Negative Control MCA691 100 Tests F

Application Based External Images

ELISA

ELISpot

Flow Cytometry

Product Specific References

References for IL-10 antibody

  1. Kwong, L.S. et al. (2002) Development of an ELISA for bovine IL-10.
    Vet Immunol Immunopathol. 85 (3-4): 213-23.
  2. Scandurra, G.M. et al. (2009) Assessment of live candidate vaccines for paratuberculosis in animal models and macrophages.
    Infect Immun. 78: 1383-9.
  3. Weiss DJ et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
    J Leukoc Biol. 83 (1): 48-55.
  4. Hamza, E. et al. (2007) Modulation of allergy incidence in icelandic horses is associated with a change in IL-4-producing T cells.
    Int Arch Allergy Immunol. 144: 325-37.
  5. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
    J Dairy Sci. 93: 2458-70.
  6. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
    Funct Integr Genomics. 10: 21-38.
  7. Parker, D.G. et al. (2010) Lentivirus-mediated gene transfer of interleukin 10 to the ovine and human cornea.
    Clin Experiment Ophthalmol. 38: 405-13.
  8. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
    Vet Res. 42:19.
  9. Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
    Clin Diagn Lab Immunol. 11: 463-72.
  10. Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.
    Vet Res. 41: 14.
  11. den Hartog, G. et al. (2011) Modulation of human immune responses by bovine interleukin-10.
    PLoSone 6: e18188
  12. Ikebuchi, R. et al. (2013) Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro.
    Vet Res. 44: 59.
  13. Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
    PLoS One. 5: e13705.
  14. Jones, G.J. et al. (2010) Simultaneous measurement of antigen-stimulated interleukin-1 beta and gamma interferon production enhances test sensitivity for the detection of Mycobacterium bovis infection in cattle.
    Clin Vaccine Immunol. 17: 1946-51.
  15. McGill, J.L. et al. (2013) Differential chemokine and cytokine production by neonatal bovine γ/δ T-cell subsets in response to viral toll-like receptor agonists and in vivo respiratory syncytial virus infection.
    Immunology. 139: 227-44.
  16. Olivier, M. et al. (2009) Capacities of migrating CD1b+ lymph dendritic cells to present Salmonella antigens to naive T cells.
    PLoS One. 7: e30430.
  17. Shu, D. et al. (2011) Diverse cytokine profile from mesenteric lymph node cells of cull cows severely affected with Johne's disease.
    Clin Vaccine Immunol. 18: 1467-76.
  18. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
    J Comp Pathol. 150 (4): 434-48.
  19. Dooley LM et al. (2015) Effect of mesenchymal precursor cells on the systemic inflammatory response and endothelial dysfunction in an ovine model of collagen-induced arthritis.
    PLoS One. 10 (5): e0124144.
  20. Rainard, P. et al. (2016) Innate and Adaptive Immunity Synergize to Trigger Inflammation in the Mammary Gland.
    PLoS One. 11 (4): e0154172.
  21. Canal AM et al. (2017) Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection.
    Res Vet Sci. 110: 34-39.
  22. Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.
    Infect Immun. 85 (2): pii: e00845-16. [Epub ahead of print]
  23. Pomeroy B et al. (2016) Impact of in vitro treatments of physiological levels of estradiol and progesterone observed in pregnancy on bovine monocyte-derived dendritic cell differentiation and maturation.
    Vet Immunol Immunopathol. 182: 37-42.
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