IL-1 Beta antibody

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Rabbit anti Rat Interleukin-1 beta:Biotin

Rabbit anti Rat Interleukin-1 beta

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAR15B E P WB 50 µg
AAR15G E FN P WB 0.1 mg
Rabbit anti Rat Interleukin-1 beta antibody recognizes rat IL-1 beta. Interleukin-1β is a 152 amino acid active pro-inflammatory cytokine produced with an additional 116 smino acid pro-peptide region. IL-1&beta has a broad mode of action, stimulating prostaglandin synthesis, neutrophil, T cell and B cell activation and collagen synthesis.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to rat IL-1 beta were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by antigen affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None Present
Preservative Stabilisers
None present.
Immunogen
Recombinant rat IL-1 beta (PRP23).
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution.

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
Q63264 Related reagents
Entrez Gene
Il1b Related reagents
GO Terms
GO:0001660 fever generation
GO:0001666 response to hypoxia
GO:0002439 chronic inflammatory response to antigenic stimulus
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005615 extracellular space
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0007568 aging
GO:0007613 memory
GO:0042493 response to drug
GO:0009408 response to heat
GO:0010193 response to ozone
GO:0010243 response to organic nitrogen
GO:0010332 response to gamma radiation
GO:0014050 negative regulation of glutamate secretion
GO:0019221 cytokine-mediated signaling pathway
GO:0030728 ovulation
GO:0032355 response to estradiol stimulus
GO:0032496 response to lipopolysaccharide
GO:0032755 positive regulation of interleukin-6 production
GO:0032874 positive regulation of stress-activated MAPK cascade
GO:0033280 response to vitamin D
GO:0033591 response to L-ascorbic acid
GO:0035176 social behavior
GO:0042060 wound healing
GO:0043065 positive regulation of apoptosis
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043278 response to morphine
GO:0043507 positive regulation of JUN kinase activity
GO:0046330 positive regulation of JNK cascade
GO:0051384 response to glucocorticoid stimulus
GO:0051781 positive regulation of cell division
GO:0070374 positive regulation of ERK1 and ERK2 cascade
Regulatory
For research purposes only

Applications of IL-1 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Immunohistology - Paraffin
Western Blotting 0.1 0.2ug/ml
ELISA 0.5ug/ml 2.0ug/ml
Functional Assays 0.09ug/ml 0.14ug/ml
Immunohistology - Paraffin
Western Blotting 0.1ug/ml 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
ELISA
This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with AAR15G as the capture reagent and PRP23 as the standard.
ELISA
This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with AAR15B as the detection reagent and PRP23 as the standard.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for IL-1 Beta antibody

  1. Girard, S. (2008) Pro-inflammatory disequilibrium of the IL-1 beta/IL-1ra ratio in an experimental model of perinatal brain damages induced by lipopolysaccharide and hypoxia-ischemia.
    Cytokine. 43: 54-62.
  2. Glatz, T. et al. (2010) Peroxisome-proliferator-activated receptors gamma and peroxisome-proliferator-activated receptors beta/delta and the regulation of interleukin 1 receptor antagonist expression by pioglitazone in ischaemic brain.
    J Hypertens. 28: 1488-97.
  3. Mahmood, J. et al. (2011) Mitigation of radiation-induced lung injury by genistein and EUK-207.
    Int J Radiat Biol. 87: 889-901.
  4. Weksler-Zangen, S. et al. (2008) Impaired glucose-stimulated insulin secretion is coupled with exocrine pancreatic lesions in the Cohen diabetic rat.
    Diabetes. 57: 279-87.
  5. Mahmood, J. et al. (2013) Mitigation of radiation-induced lung injury with EUK-207 and genistein: effects in adolescent rats.
    Radiat Res. 179 (2): 125-34.
  6. Cho, G.S. et al. (2013) N-Methyl-D-aspartate receptor antagonists memantine and MK-801 attenuate the cerebral infarct accelerated by intracorpus callosum injection of lipopolysaccharides.
    Neurosci Lett. 538: 9-14.
  7. Savard, A. et al. (2013) Involvement of neuronal IL-1β in acquired brain lesions in a rat model of neonatal encephalopathy.
    J Neuroinflammation. 10: 110.
  8. Aharon-Hananel G et al. (2015) Antidiabetic Effect of Interleukin-1β Antibody Therapy Through β-Cell Protection in the Cohen Diabetes-Sensitive Rat.
    Diabetes. 64 (5): 1780-5.
  9. Bergeron, J. et al. (2016) Activation of the IL-1β/CXCL1/MMP-10 axis in chorioamnionitis induced by inactivated Group B Streptococcus.
    Placenta. 47: 116-23.
  10. Alizadeh A et al. (2017) Neuregulin-1 positively modulates glial response and improves neurological recovery following traumatic spinal cord injury.
    Glia. Apr 29. [Epub ahead of print]
  11. Calveley, V.L. et al. (2010) Genistein can mitigate the effect of radiation on rat lung tissue.
    Radiat Res. 173 (5): 602-11.
  12. Miyai, H. et al. (2017) Topical application of ointment containing 0.5% green tea catechins suppresses tongue oxidative stress in 5-fluorouracil administered rats.
    Arch Oral Biol. 82: 247-55.
  13. Kataria, H. et al. (2017) Neuregulin-1 promotes remyelination and fosters a pro-regenerative inflammatory response in focal demyelinating lesions of the spinal cord.
    Glia. Nov 17 [Epub ahead of print].
  14. Dyck, S. et al. (2018) Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTPσ receptors promotes a beneficial inflammatory response following spinal cord injury.
    J Neuroinflammation. 15 (1): 90.