Fluorophores for Flow Cytometry
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Fluorophores and Viability Dyes for Flow Cytometry
Overview
Flow cytometry generally requires bright fluorophores that are excitable by one laser with a narrow emission wavelength to allow multicolor panel building. Below is a handy guide to the range of fluorophores Bio-Rad offers, including our new StarBright Dyes, that are suitable for flow cytometry, grouped by the laser used to excite them.
Additional fluorophore information can be found on specific webpages organized by the laser that excites them. This includes the maximal emission wavelength, relative brightness, ZE5 Cell Analyzer filter used for detection, similar or incompatible fluorophores, and Bio-Rad antibodies available conjugated to them, helping you select the right fluorophore for your experiment.
For more information on which fluorophores are the most suitable for your experiment, try our fluorescent spectraviewer or download our poster showing common fluorophores used in flow cytometry.
Fluorophores excited by the 355 nm (Ultraviolet) Laser
- StarBright UltraViolet 400
- StarBright UltraViolet 445
- StarBright UltraViolet 510
- StarBright UltraViolet 575
- StarBright UltraViolet 605
- StarBright UltraViolet 665
- StarBright UltraViolet 740
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StarBright UltraViolet 795
Fluorophores Excited by the 405 nm (Violet) Laser
- Pacific Blue
- StarBright Violet 440
- StarBright Violet 475
- StarBright Violet 515
- Amethyst Orange
- StarBright Violet 570
- StarBright Violet 610
- StarBright Violet 670
- StarBright Violet 710
- StarBright Violet 760
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StarBright Violet 790
Fluorophores Excited by the 488 nm (Blue) Laser
- FITC
- Alexa Fluor 488
- PE
- PE-Alexa Fluor647
- PE-Cy5
- PE-Cy5.5
- PE-Alexa Fluor750
- PE-Cy7
- PerCP
- StarBright Blue 580
- StarBright Blue 615
- StarBright Blue 675
- StarBright Blue 700
- StarBright Blue 765
-
StarBright Blue 810
Fluorophores Excited by the 561 nm (Yellow) Laser
- PE
- PE-Alexa Fluor647
- PE-Cy5
- PE-Cy5.5
- PE-Alexa Fluor750
- PE-Cy7
- StarBright Yellow 575
- StarBright Yellow 605
- StarBright Yellow 665
- StarBright Yellow 720
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StarBright Yellow 800
Fluorophores Excited by the 640 nm (Red) Laser
- Alexa Fluor 647
- APC
- Alexa Fluor 700
- StarBright Red 670
- StarBright Red 715
- StarBright Red 775
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StarBright Red 815
For more information about fluorophores and immunophenotyping, refer to our flow cytometry resources.
Click on the relevant links below to find more in-depth information on each topic and view our popular flow cytometry basics guide.