IL-21 antibody

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Rabbit anti Mouse Interleukin-21:Biotin

Rabbit anti Mouse Interleukin-21

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAM61B E WB 50 µg
AAM61 E WB 0.1 mg
Rabbit anti Mouse Interleukin-21 antibody recognizes mouse interleukin-21 (IL-21), a pleiotropic cytokine and member of the IL-15/IL-21 family, secreted by stimulated CD4+ T cells, which enhances stimulated B-cell proliferation/differentiation, the proliferation of bone marrow progenitor cells, and increases the activity of NK cells and disease-specific CD8+ T-cells.

IL-21 signals through binding to the specific type I cytokine receptor IL-21R, coupled with the common cytokine receptor g chain (gc), initiating the activation of the JAK/STAT signalling pathway.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised.
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antiserum to mouse IL-21 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Immunogen
Recombinant mouse IL-21
Approx. Protein Concentrations
IgG concentration 0.1mg/ml after reconstitution
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
Q9ES17 Related reagents
Entrez Gene
Il21 Related reagents
GO Terms
GO:0001783 B cell apoptosis
GO:0002729 positive regulation of natural killer cell cytokine production
GO:0005125 cytokine activity
GO:0005126 cytokine receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0032733 positive regulation of interleukin-10 production
GO:0032825 positive regulation of natural killer cell differentiation
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0045579 positive regulation of B cell differentiation
GO:0045954 positive regulation of natural killer cell mediated cytotoxicity
Regulatory
For research purposes only

Applications of IL-21 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
ELISA 0.5 2.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This antibody may be used in an indirect ELISA or as a detection reagent in a sandwich ELISA with AAM61 as the capture antibody.
ELISA
This antibody may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with AAM61B as the detection antibody.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*