IL-4 antibody | CC313

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Mouse anti Bovine Interleukin-4

Product Type
Monoclonal Antibody
Clone
CC313
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2371 E 0.5 mg
Mouse anti Bovine interleukin-4, clone CC313 is specific for bovine interleukin 4 (IL-4), also known as B-cell stimulatory factor 1. Bovine interleukin-4 is a 15 kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6 kDa active cytokine, the apparent Mw may be altered by glycosylation (Heussler et al. 1992).

IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses.

Mouse anti Bovine interleukin-4, clone CC313 has been used successfully as a coating antibody in a sandwich ELISA with biotinylated Mouse anti Bovine IL-4 antibody, clone CC314 (MCA2372B) as a detection reagent both with bovine (Stabel et al. 2011) and ovine samples (Olivier et al. 2012). Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples (Marinaro et al. 2012).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Recombinant bovine IL-4.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells form immunised BALB/c mice were fused with cells of the SP2/O myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P30367 Related reagents
Entrez Gene
IL4 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005136 interleukin-4 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0030890 positive regulation of B cell proliferation
GO:0042113 B cell activation
GO:0043066 negative regulation of apoptosis
GO:0045348 positive regulation of MHC class II biosynthetic process
GO:0045671 negative regulation of osteoclast differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048295 positive regulation of isotype switching to IgE isotypes
GO:0048304 positive regulation of isotype switching to IgG isotypes
Regulatory
For research purposes only

Applications of IL-4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 2ug/ml 5ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
ELISA
This reagent may be used as a capture antibody in sandwich ELISA assays for bovine IL-4 in combination with MCA2372B as a detection reagent, and recombinant bovine IL-4 (PBP006) as a standard.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

ELISA

Product Specific References

References for IL-4 antibody

  1. Hope, J.C. et al. (2005) Development of detection methods for ruminant interleukin (IL)-4.
    J Immunol Methods. 301 (1-2): 114-23.
  2. Abbott, J.R. et al. (2005) Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale.
    J Immunol. 174 (11): 6702-15.
  3. Sipka, A. et al. (2013) Prednisolone and cefapirin act synergistically in resolving experimental Escherichia coli mastitis
    J Dairy Sci. 96: 4406-18.
  4. Stabel, J.R. et al. (2013) Disparate Host Immunity to Mycobacterium avium subsp. paratuberculosis Antigens in Calves Inoculated with M. avium subsp. paratuberculosis, M. avium subsp. avium, M. kansasii and M. bovis.
    Clin Vaccine Immunol. 20: 848-57.
  5. Stabel, J.R. et al. (2011) Mediation of host immune responses after immunization of neonatal calves with a heat-killed Mycobacterium avium subsp. paratuberculosis vaccine.
    Clin Vaccine Immunol. 18: 2079-89.
  6. Dacal, V. et al. (2009) Local and systemic cytokine responses during larval penetration in cattle experimentally infested with Hypoderma lineatum (Diptera: Oestridae).
    Vet Immunol Immunopathol. 131: 59-64.
  7. Olivier, M. et al. (2012) Capacities of migrating CD1b+ lymph dendritic cells to present Salmonella antigens to naive T cells.
    PLoS One. 7: e30430.
  8. Marinaro, M. et al. (2012) Antigen-specific IFN-gamma and IL-4 production in caprine herpesvirus infected goats.
    Res Vet Sci. 93: 662-7.
  9. Panadero, R. et al. (2009) Immunomodulatory effect of Hypoderma lineatum antigens: in vitro effect on bovine lymphocyte proliferation and cytokine production.
    Parasite Immunol. 31: 72-7.
  10. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
    J Comp Pathol. 150 (4): 434-48.
  11. Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.
    Infect Immun. 85 (2): pii: e00845-16. [Epub ahead of print]