IL-4 antibody | CC313
Mouse anti Bovine Interleukin-4 antibody, clone CC313 (MCA2371) used as a capture reagent for the detection of interleukin-4 in supernatants of cultured bovine cells using a sandwich ELISA together with biotinylated clone CC314 (MCA2372B) as a detection reagent.
Image caption:
CD1b+ L-DCs are efficient APC for the priming of allogeneic naive T cells. Sorted CD1b+ L-DCs were incubated at different ratios with 105 allogeneic CD4+ T cells (A) or CD62L+ T cells (C), and tested for proliferation by [3H]-thymidine incorporation after six days of culture. Data express the median (quartiles) of counts per minute (cpm) from triplicates for each condition. After three days of culture, supernatants were sampled and IFN-γ (black bars) and IL-4 (grey bars) production analysed by ELISA (B, D). The results shown are from one representative experiment out of four performed on different sheep (A) or out of two performed on one sheep(C).
From: Olivier M, Foret B, Le Vern Y, Guilloteau LA (2012)
Capacities of Migrating CD1b+ Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.
PLoS ONE 7(1): e30430.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine interleukin-4 antibody, clone CC313 (MCA2371) used to detect IL-4 expressing ovine cells in pulmonary tissue sections by immunofluorescence.
Image caption:
Immunostaining of IL-4 and IL-13 in lung tissues following allergen challenge, showing (a) negative staining (isotype-matched control Ab), (b) IL-4+ cells (red, arrows), (c) CD45+ leukocyte (red) staining, and (d) CD45+ (red) and IL-13+ (green, arrows) staining; the arrow shows an IL-13 expressing leukocyte (IL-13+CD45+) and the arrow head shows an IL-13 expressing non-leukocyte (IL-13+CD45−). Representative sections were taken from the same lung lobe of a sheep, collected post-mortem at 48 h post-HDM challenge. All slides were counterstained with DAPI (blue) when mounted in Mowiol (Original magnification × 400)
From: Liravi B, Piedrafita D, Nguyen G, Bischof RJ.
Dynamics of IL-4 and IL-13 expression in the airways of sheep following allergen challenge.
BMC Pulm Med. 2015 Sep 11;15:101.
doi: 10.1186/s12890-015-0097-9.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Bovine interleukin-4 antibody, clone CC313 (MCA2371) used to evaluate IL-4 levels in bronchiolar lavage samples by ELISA
Image caption:
Cytokine protein levels over time (0–48 h) showing (a) IL-4, (b) IL-13, (c) IL-6, (d) IL-10 and (E) TNF-α in BAL fluid following saline and HDM bronchial challenges. Data presented as means ± SD for n = 10 sheep (*denotes significant difference between HDM and saline-challenged lobes at the corresponding time point: *p < 0.05, **p <0.01, ***p <0.001, ****p <0.0001; #denotes significant difference between time points: #p <0.05, ##p <0.01, ###p <0.001, ####p <0.0001).
From: Liravi B, Piedrafita D, Nguyen G, Bischof RJ.
Dynamics of IL-4 and IL-13 expression in the airways of sheep following allergen challenge.
BMC Pulm Med. 2015 Sep 11;15:101.
doi: 10.1186/s12890-015-0097-9.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
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- Product Type
- Monoclonal Antibody
- Clone
- CC313
- Isotype
- IgG2a
- Specificity
- IL-4
Antibody Quality and Performance Guarantee
| Mouse anti Bovine interleukin-4, clone CC313 is specific for bovine interleukin 4 (IL-4), also known as B-cell stimulatory factor 1. Bovine interleukin-4 is a 15 kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6 kDa active cytokine, the apparent MW may be altered by glycosylation (Heussler et al. 1992). IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses. Mouse anti Bovine interleukin-4, clone CC313 has been used successfully as a coating antibody in a sandwich ELISA with biotinylated Mouse anti Bovine IL-4 antibody, clone CC314 (MCA2372B) as a detection reagent both with bovine (Stabel et al. 2011) and ovine samples (Olivier et al. 2012). Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples (Marinaro et al. 2012). |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant bovine IL-4.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells form immunized BALB/c mice were fused with cells of the SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
2ug/ml | 5ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
- ELISA
- Mouse anti Bovine interleukin-4 antibody, clone CC313 may be used as a capture antibody in sandwich ELISA assays for bovine IL-4 in combination with MCA2372B as a detection reagent, and recombinant bovine IL-4 (PBP006) as a standard.
References for IL-4 antibody
-
Hope, J.C. et al. (2005) Development of detection methods for ruminant interleukin (IL)-4.
J Immunol Methods. 301 (1-2): 114-23. -
Abbott, J.R. et al. (2005) Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale.
J Immunol. 174 (11): 6702-15. -
Sipka, A. et al. (2013) Prednisolone and cefapirin act synergistically in resolving experimental Escherichia coli mastitis
J Dairy Sci. 96: 4406-18. -
Stabel, J.R. et al. (2013) Disparate Host Immunity to Mycobacterium avium subsp. paratuberculosis Antigens in Calves Inoculated with M. avium subsp. paratuberculosis, M. avium subsp. avium, M. kansasii and M. bovis.
Clin Vaccine Immunol. 20: 848-57. -
Stabel, J.R. et al. (2011) Mediation of host immune responses after immunization of neonatal calves with a heat-killed Mycobacterium avium subsp. paratuberculosis vaccine.
Clin Vaccine Immunol. 18: 2079-89. -
Dacal, V. et al. (2009) Local and systemic cytokine responses during larval penetration in cattle experimentally infested with Hypoderma lineatum (Diptera: Oestridae).
Vet Immunol Immunopathol. 131: 59-64. -
Olivier, M. et al. (2012) Capacities of migrating CD1b+ lymph dendritic cells to present Salmonella antigens to naive T cells.
PLoS One. 7: e30430. -
Marinaro, M. et al. (2012) Antigen-specific IFN-gamma and IL-4 production in caprine herpesvirus infected goats.
Res Vet Sci. 93: 662-7.
View The Latest Product References
-
Panadero, R. et al. (2009) Immunomodulatory effect of Hypoderma lineatum antigens: in vitro effect on bovine lymphocyte proliferation and cytokine production.
Parasite Immunol. 31: 72-7. -
Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
J Comp Pathol. 150 (4): 434-48. -
Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.
Infect Immun. 85 (2): pii: e00845-16. [Epub ahead of print] -
Liravi, B. et al. (2015) Dynamics of IL-4 and IL-13 expression in the airways of sheep following allergen challenge.
BMC Pulm Med. 15: 101. -
Rodrigues, V. et al. (2017) Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry.
Cytometry A. 91 (9): 901-7. -
Stabel, J.R. et al. (2021) Comparative cellular immune responses in calves after infection with Mycobacterium avium. subsp. paratuberculosis., M. avium. subsp. avium., M. kansasii. and M. bovis..
Vet Immunol Immunopathol. 237: 110268.
- RRID
- AB_2249178
- UniProt
- P30367
- Entrez Gene
- IL4
- GO Terms
- GO:0005125 cytokine activity
- GO:0005136 interleukin-4 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0030890 positive regulation of B cell proliferation
- GO:0042113 B cell activation
- GO:0043066 negative regulation of apoptosis
- GO:0045348 positive regulation of MHC class II biosynthetic process
- View More GO Terms
- GO:0045671 negative regulation of osteoclast differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048295 positive regulation of isotype switching to IgE isotypes
- GO:0048304 positive regulation of isotype switching to IgG isotypes
MCA2371
1605
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