StarBright Dye Technical Notes
Overview
Flow cytometry is a complex application and navigating new protocols can be tricky. Our comprehensive technical notes are designed to help you understand how to successfully perform experiments and avoid common pitfalls. With detailed protocols, troubleshooting tips, and expert insights, you'll find everything you need to achieve accurate and reliable results.
We have a diverse range of technical notes to assist and guide you in generating high-quality data from your flow cytometry assays using antibodies conjugated to StarBright™ Dyes and other fluorophores.
These include immunophenotyping panels ranging from a large 43-color human panel to a smaller 10-color porcine panel. They offer detailed protocols, gating strategies, and best practices for designing and executing your own panels.
In addition to immunophenotyping, we also have technical notes focusing on other aspects of flow cytometry. For example, designing and using premixed cocktails, and how StarBright Dye–conjugated antibodies are compatible with compensation beads, allowing you to use beads for your single-stained controls in your assays with confidence.
Access all technical notes by selecting the relevant section.
Human Immunophenotyping Panels
Panel design, protocol, gating strategy, and results from a large multiplexing panel containing StarBright Dye and traditional conjugated antibodies acquired on a Cytek Aurora (Cytek Biosciences).
A 27-color panel acquired on the ZE5 Cell Analyzer, includes information on panel design and gating strategy.
Validated Method to Measure Multiple Parameters in Stimulated Human Peripheral Blood
A detailed method for simultaneously measuring cell proliferation, viability, and activation status using compatible products.
Highlights the benefits of including StarBright Dye–conjugated antibodies in a human immunophenotyping panel. Includes technical details and information on panel design.
Veterinary Immunophenotyping Panels
An immunophenotyping panel to identify major bovine blood cell populations, including T cell subsets, B cells, NK cells, and monocytes.
10-Color Canine Immunophenotyping Panel Featuring StarBright™ Dye–Conjugated Antibodies.
An immunophenotyping panel to identify T cells, B cells, NK cells, monocytes, and granulocytes in canine peripheral blood.
A 10-color porcine panel enabling the identification of T cells, B cells, NK cell, monocytes, and granulocytes in one panel.
Using StarBright Dyes in Premixed Panels
Describes the 12-month stability of StarBright Dye and traditional conjugated antibodies in a premixed cocktail, with tips on how to design your own premixed cocktails.
Using StarBright Dyes with Compensation Beads
Comparison of Antibody-Capture Compensation Beads and StarBright Dyes
Demonstrates the compatibility of StarBright Dye–conjugated antibodies with common compensation beads.
Resources for Flow Cytometry
- Flow Cytometry Hub
- Protocols
- Multicolour Panel Building
- Spectraviewer
- Flow Cytometry Design Tool
- Bio-Rad Academy — Fundamentals of FC
Resources for Flow Cytometry
Latest Resources
Fluorophores and Viability Dyes for Flow Cytometry
Easily select your ideal fluorophore with this handy reference guide that helps you quickly compare characteristics such as relative brightness and maximal emission.
Bio-Rad Academy — Fundamentals of Flow Cytometry
Enroll in Bio-Rad’s Fundamentals of Flow Cytometry Series, a free, online course that covers the basics of flow cytometry and experimental best practices.