Cystatin C antibody | E01-8H8

Mouse anti Human Cystatin C:Biotin

Product Type
Monoclonal Antibody
Clone
E01-8H8
Isotype
IgG1
Specificity
Cystatin C

Product Code Applications Pack Size List Price Your Price Qty
MCA6227B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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Mouse anti Human cystatin C antibody, clone E01-8H8 , recognizes cystatin C, a small non-glycosylated 13.3 kDa basic cysteine inhibitor and a member of the Cystatin superfamily. Cystatin C is widely expressed in all nucleated cells in the body. It is also secreted from cells into circulation and other biological fluids including urine, seminal fluid, saliva and cerebrospinal fluid (Mussap & Plebani 2004).

Cystatin C specifically inhibits cysteine proteases such as papain, legumain and the cathepsins B, H, K, L and S. Through the regulation of cysteine proteases, it plays a role in various biological processes including immune responses, protein turnover, phatocytosis, antigen presentation and apoptosis (Zi & Xu 2018). When released into the blood, cystatin C is filtered by the glomerous and metabolised by proximal tubular cells. Because of this filtration of cystatin C by the kidneys, this inhibitor is commonly used clinically as a biomarker of kidney function. (Zi & Xu 2018). Within immunological processes, cystatin C regulates proteolytic processing of pro-granzymes, MHC II antigen presentation and maturation of dendritic cells (Pierre & Mellman 1998).

Cystatin C is encoded by the gene CST3 and mutations of this gene are responsible for the heritable disease, cerebral amyloid angiopathy (Jurczak et al. 2016).

The biotinylated Mouse anti Human cystatin C antibody, clone E01-8H8 (MCA6227B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human cystatin C antibody, clone B01-8B1 (MCA6226GA) as the capture antibody.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length cystatin C (Ser27-Ala146)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human Cystatin C MCA6226GA E 0.1 mg loader
List Price Your Price
loader
Description Mouse anti Human Cystatin C

UniProt
P01034
Entrez Gene
CST3
GO Terms
GO:0001540 beta-amyloid binding
GO:0002020 protease binding
GO:0006952 defense response
GO:0004869 cysteine-type endopeptidase inhibitor activity
GO:0005615 extracellular space
GO:0010703 negative regulation of histolysis
GO:0010711 negative regulation of collagen catabolic process
GO:0010716 negative regulation of extracellular matrix disassembly
GO:0043206 fibril organization
GO:0060311 negative regulation of elastin catabolic process
GO:0060313 negative regulation of blood vessel remodeling
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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