Mouse anti Human PDGF-BB antibody, clone AB06-1E10,
recognizes the homodimer of platelet-derived growth factor subunit B (PDGF-B) also known as PDGF-2, PDGF beta chain and PDGF beta polypeptide. PDGF-BB is a member of the PDGF growth factor family which is made up of 4 isoforms (A, B, C and D). PDGF-BB is a homodimer of the B isoform which is able to bind all three of the PDGF receptors to trigger various signaling pathways including Ras-MAPK, PI3K, PLCγ, and JAK (Evrova & Buschmann 2017
). Activation of these pathways lead to transcription of PDGF target genes playing roles in embryogenesis, and in adults under physiological conditions, roles in tissue repair, wound healing and maturation of blood vessels and white adipocytes (Saplacan et al. 2017
The purified Mouse anti Human PDGF-BB antibody, clone AB06-1E10 (MCA6264GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human PDGF-BB antibody, clone AB13-3G6 (MCA6265B
) as the detection antibody.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of PDGF-BB antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.