StarBright Yellow Dyes

SBY575 is the first of our new StarBright Yellow Dye series. It is a bright 561 nm excitable dye emitting at 579 nm with a narrow excitation and emission profile.

Although it is not as bright as PE, it is still a bright dye (Figure 3) with the benefit of not being excited to the same extent by the 488 nm laser.

This results in less spillover when building panels containing 488 nm excitable dyes in conventional flow cytometry. As it has a unique spectra, StarBright Yellow 575 Dye can be used with PE in full spectrum flow cytometry.
 

SBY605 is a bright 561 nm excitable dye with a unique and narrow emission that unlike PE-tandems with similar emissions, is not excited by the 488 nm laser.

Suitable for use in conventional and full spectrum flow cytometry and compatible with other StarBright Dyes without the requirement for a special buffer, it is as bright as PE-Dazzle594 when excited by the 561 nm laser (Figure 4).

SBY605 is not a traditional tandem dye so unlike PE-based tandems can be fixed in PFA or alcohol-based fixatives with no loss of signal or changes to the spectral profile giving you reduced spillover and more flexibility in your experiments.
 

SBY665 is a bright 561 nm laser excitable dye emitting at 670 nm with a unique and narrow emission that, unlike PE-tandems with similar emissions such as PE-Cy5 and PE-A647, is not excited by the 488 nm laser to the same extent, resulting in lower spillover and compensation.

SBY665 is suitable for use in conventional and full spectrum flow cytometry with a unique spectra, and is compatible with other StarBright Dyes without the requirement for a special buffer. It is brighter than PE-A647, but not as bright as the very bright dye PE-Cy5, when excited by the 561 nm laser (Figure 5).

SBY665 is not a traditional tandem dye so, unlike PE-based tandems, it can be fixed in PFA or alcohol-based fixatives with no loss of signal and minimal changes to the spectral profile giving you reduced spillover and more flexibility in your experiments.
 

SBY720 is a bright 561 nm excitable dye with a unique and narrow emission. It can be used in place of PE-Cy5.5 and PE-A700 in multicolor panels as it is brighter, has reduced 488 nm excitation, and is fixable in PFA and alcohol-based buffers without loss of signal or changes to the spectra (Figure 5).

Furthermore, as it is not a traditional tandem dye it is not prone to changes in emission over time due to acceptor and donor uncoupling, giving you reproducible data.

Like all StarBright Dyes, SBY720 can be multiplexed with other StarBright Dyes or other fluorophores without the need for a special buffer and can be premixed in a panel and stored at 4⁰C for over a month without changes to staining.
 

SBY800 is a bright 561 nm laser excitable dye, which emits at 788 nm with a unique and narrow emission that, unlike PE-Cy7, is not excited by the 488 nm laser to the same extent.

SBY800 is a bright 561 nm laser excitable dye, which emits at 788 nm with a unique and narrow emission that, unlike PE-Cy7, is not excited by the 488 nm laser to the same extent.

SBY800 is not a traditional tandem dye so, unlike PE-based tandems, can be fixed in PFA or alcohol-based fixatives with minimal loss of signal or changes to the spectral profile giving you reduced spillover and more flexibility in your experiments.