StarBright Yellow Dyes

Excitable by the 561 nm laser, StarBright Yellow (SBY) Dyes, are perfect to replace existing dyes in your flow cytometry panels and expand your capabilities across the yellow laser.

Designed to be bright enough to resolve most populations, with reduced excitation by the 488 nm laser, compared to PE and PE-tandems, StarBright Yellow Dyes should be your first choice in panel design.

StarBright Yellow Dye Range

StarBright Dye

Max Ex, nm

Max Em, nm

Brightness 1-5

5-laser ZE5 Cell Analyzer Optimal Filter

3/4-Laser ZE5 Cell Analyzer Filter

Comparison Dye

Launch Date

Flier

StarBright Yellow 575

548

579

3

583/30

577/15

PE

Available now

 

StarBright Yellow 605

572

606

3

615/24

615/24

PE-Dazzle594, PE-DyL594

Available now

 

StarBright Yellow 665

554

670

3

670/30

670/30

PE-Cy5, PE-A647

Coming soon

 

StarBright Yellow 720

549

719

4

720/60

720/60

PE-Cy5.5, PE-Fire700

Available now

 

StarBright Yellow 800

549

788

4

 

750LP

750LP

PE-Cy7, PE-A750

Coming soon

 

Abbreviations: Axxx, Alexa Fluor; Cy, cyanine; DyL, DyLight; PE, Phycoerythrin

Fig. 1. Excitation and emission spectra of StarBright Yellow Dyes


Fig. 1. Excitation and emission spectra of StarBright Yellow Dyes 

Fig. 2. Comparison of the effects of fixation.

Fig. 2. Comparison of the effects of fixation. Human blood was stained with CD4SBY575 or CD4PE and the effects of methanol fixation compared to fresh stained samples are shown.

StarBright Yellow Dyes are the perfect replacements for PE and PE-tandems in your flow cytometry experiments.

Bright, with reproducible unique spectra that can be fixed in PFA or alcohol-based fixatives with minimal performance changes, these dyes are suitable for both conventional and spectral flow cytometry (Figure 2).

With reduced excitation by the 488 nm laser compared to PE and PE tandems, StarBright Yellow Dyes have reduced spillover and spreading, giving you better resolution in multicolor panels.


Fig. 3. Brightness comparison.

Fig. 3. Brightness comparison. Human peripheral blood was stained with CD4 SBY575 (MCA1267SBY575) (red), or CD4 PE (blue) and analyzed on the ZE5 Cell Analyzer detected using the 577/15 filter. All antibodies were titrated prior to use to determine the optimal concentration.

StarBright Yellow (SBY575) Dye

SBY575 is the first of our new StarBright Yellow Dye series. It is a bright 561 nm excitable dye emitting at 579 nm with a narrow excitation and emission profile.

Although it is not as bright as PE, it is still a bright dye (Figure 3) with the benefit of not being excited to the same extent by the 488 nm laser.

This results in less spillover when building panels containing 488 nm excitable dyes in conventional flow cytometry. As it has a unique spectra, StarBright Yellow 575 Dye can be used with PE in full spectrum flow cytometry.

StarBright Yellow Dyes can be used successfully in combination with other StarBright Dyes and traditional fluorophores. Figure 4 shows how they can be used to identify common and rare subsets including naïve and memory status in human peripheral blood.


Fig. 4. Immunophenotyping panel including StarBright Yellow Dye conjugated antibodies


Fig. 4. Immunophenotyping panel including StarBright Yellow Dye conjugated antibodies. Red blood cell lysed human peripheral blood was stained with 11 antibodies and a L/D marker. StarBright Yellow Dyes used in this panel were SBY575, SBY605, SBY665, and SBY800. Cells were also gated on live, single cells (not shown).

StarBright Yellow Antibodies

    DescriptionSpecificityTargetFormatHostIsotypeClone Applications Citations Product Type Code Validation Types

    Associated Reading

    For more information on how these products can improve the effectiveness of your flow cytometry experiments, please take a look at our StarBright Dyes page.

    Useful information about fluorescent dyes and immunophenotyping can be found in our flow cytometry explained knowledge hub. Alternatively, feel free to explore the associated resource links below, for more in-depth information about each cited topic. If you are interested in learning the fundamentals of this application, please view our flow cytometry basics guide.