HuCAL® Recombinant Monoclonal Antibody Generation Service
A personal, no obligation quotation for a HuCAL custom monoclonal antibody generation project
Tel: +49 (0) 89 80 90 95 45
Fax: +49 (0) 89 80 90 95 50
Office: Zeppelinstr. 4, 82178 Puchheim, Germany
We understand that design and optimization of immunoassays is complex and time consuming. Having an antibody in only one format limits your options, and the resulting assay may not answer all your questions. Producing several different conjugates or alternative formats can be tedious and costly, and delay your progress.
We can help you overcome these challenges by providing more sophisticated antibodies. We have incorporated SpyTag-SpyCatcher technology into our custom HuCAL® recombinant antibody generation service, to make 'TrailBlazer Antibodies'. The service provides you with an extensive antibody toolbox that enables rapid development of robust assays, leading to results that advance your research.
Fig. 1. Convert one monovalent Fab antibody with SpyTag (center) to multiple formats using a variety of SpyCatchers.
When the SpyTag peptide and SpyCatcher protein are mixed, a spontaneous reaction results in an irreversible covalent isopeptide bond forming between them. We have added the SpyTag peptide as a tag to the HuCAL Fab antibody, and made a set of SpyCatchers to couple to the Fab. The Catchers enable site-directed conjugation to HRP or biotin, and the formation of bivalent Fab and Ig-like constructs with human, mouse, and rabbit isotypes.
a. Generate in a new custom antibody project, or
b. Convert an existing custom HuCAL clone to SpyTag format
The degree of labeling of Catchers is measured and controlled between batches, and coupling Fab to Catcher is site-specific at a 1:1 ratio using SpyTag-SpyCatcher technology. This results in consistency of labeling between batches and avoids conjugation occurring at the active site.
This innovative combination of SpyTag and HuCAL technologies brings you:
Rapid generation of highly specific Fab antibodies in as little as 8 weeks
Isolation of antibodies against virtually any type of antigen, for instance proteins, peptides,
biologics, low immunogenic antigens, and toxins
Fast, flexible format conversion using SpyTag technology, +1 week
Site-directed conjugation with consistent degree of labeling using SpyTag technology
Sequenced ─ antibody identity known, long-term secure supply guaranteed
Follow these steps to get your customized TrailBlazer Antibodies:
R&D Team Leader Dr Francisco Ylera explains how we have incorporated SpyTag-SpyCatcher technology into the custom HuCAL antibody generation service.