TrailBlazer Antibodies

Be the first to know when we launch new products and resources to help you achieve more in the lab.

Sign Up Now ▸

s

HuCAL® Recombinant Monoclonal Antibody Generation Service

Request or Download a Copy ▸

s

Tel: +49 (0) 89 80 90 95 45
Fax: +49 (0) 89 80 90 95 50
Office: Bio-Rad AbD Serotec GmbH, Campus Neuried, Anna-Sigmund-Str. 5, 82061 Neuried, Germany

Email Us ▸

s

Improve Experimental Flexibility and Achieve Reproducible Results

We understand that design and optimization of immunoassays is complex and time consuming. Having an antibody in only one format limits your options, and the resulting assay may not answer all your questions. Producing several different conjugates or alternative formats can be tedious and costly, and delay your progress.

We can help you overcome these challenges by providing more sophisticated antibodies. We have incorporated SpyTag-SpyCatcher technology into our custom HuCAL® recombinant antibody generation service, to make TrailBlazer Antibodies. The service provides you with an extensive antibody toolbox that enables rapid development of robust assays, leading to results that advance your research.

Watch this short animation to discover how antibody phage display and SpyTag technology have been combined to make TrailBlazer Antibodies.

How SpyTag-SpyCatcher Technology Works with Custom HuCAL Antibodies

When the SpyTag peptide and SpyCatcher protein are mixed, a spontaneous reaction results in an irreversible covalent isopeptide bond forming between them. We have added the SpyTag peptide as a tag to the HuCAL Fab antibody, and made a set of SpyCatchers to couple to the Fab. The Catchers enable site-directed conjugation to HRP or biotin, and the formation of bivalent Fab and Ig-like constructs with human, mouse, and rabbit isotypes.

The process is simple:
 

1. Start with a monovalent Fab with a SpyTag

a. Generate in a new custom antibody project, or
b. Convert an existing custom HuCAL clone to SpyTag format

2. Select from a variety of Catchers for coupling to the Fab to make:

• Monovalent labeled with HRP or biotin
• Bivalent (unlabeled)
• Bivalent labeled with HRP, biotin, or RPE
• Synthetic Ig-like human IgG1 labeled with HRP or biotin
• Ig-like human IgG1, 2, 3, 4, IgA (unlabeled)
• Ig-like mouse IgG2a or rabbit IgG (unlabeled)
   

The degree of labeling of Catchers is measured and controlled between batches, and coupling Fab to Catcher is site-specific at a 1:1 ratio using SpyTag-SpyCatcher technology. This results in consistency of labeling between batches and avoids conjugation occurring at the active site.

Listen to this interview to learn how SpyTag-SpyCatcher technology has been introduced into the custom HuCAL antibody generation process, enabling site-specific antibody conjugation and rapid assembly of different antibody formats.

The advantages this innovation brings to the development of pharmacokinetic and anti-drug antibody bridging ELISAs are explained in detail.

Imagine Having Such a Variety of Products in Your Hands from the Outset

• Time saving - access to multiple antibody formats at once
• Enormous flexibility - test different formats to optimize your assay
• Better experimental reproducibility - consistently label antibodies
• Robust data – have confidence in your results

This innovative combination of SpyTag and HuCAL technologies brings you:

    Rapid generation of highly specific Fab antibodies in as little as 8 weeks
    Isolation of antibodies against virtually any type of antigen, for instance proteins, peptides,
         biologics, low immunogenic antigens, and toxins
    Fast, flexible format conversion using SpyTag technology
    Site-directed conjugation with consistent degree of labeling using SpyTag technology
    Sequenced - antibody identity known, long-term secure supply guaranteed

Next Steps to Achieving Superior Assays

Follow these steps to get your customized TrailBlazer Antibodies:

1.  Get all of the details in the technical article [Download] 
2.  Discuss your project with a custom antibody specialist [Schedule a call] 
3.  Work with us to design the antibodies you need
4.  Order your custom antibody project
5.  Receive your antibodies within 3 months and get your assays started
6.  Order SpyCatchers to switch antibody format or conjugate to alternative labels yourself

Resources

TrailBlazer Antibody Custom Services
 

Get more details about SpyTag technology, how it works with our antibody generation service, and see data for different experimental applications.

Download PDF

Recombinant Antibodies with SpyTag
Technology
 

Learn how SpyTag technology has been incorporated with
custom recombinant antibody generation to make
TrailBlazer Antibodies, enabling switching between multiple
format and conjugation options in less than one hour.

Listen to Webinar

Transform Your Bioanalytical Assay
Development with TrailBlazer Antibodies
 

Learn how SpyTag technology has been incorporated with
custom recombinant antibody generation to make
"TrailBlazer Antibodies".

Listen to Webinar

Faster Generation of Anti-Drug Antibodies Using SpyTag Technology
 

This poster explains how recombinant antibodies with a SpyTag are combined with SpyCatchers to provide antibodies in multiple formats for fast assay development. Learn how site-directed conjugation of the detection antibody leads to increased assay sensitivity.

Download PDF

References

• Keeble et al. (2017). Evolving accelerated amidation by SpyTag/SpyCatcher to analyze membrane dynamics. Angew Chem Int Ed Engl. 56(52), 16521-16525.
• Zakeri et al. (2012). Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. PNAS 109(12), 690-697.