Complement Factor H antibody | C06-2F3
Mouse anti Human Complement Factor H
- Product Type
- Monoclonal Antibody
- Clone
- C06-2F3
- Isotype
- IgG1
- Specificity
- Complement Factor H
| Mouse anti Human complement Factor H antibody, clone C06-2F3, recognizes, complement factor H, also known as H Factor1. Complement factor H is a major regulatory protein of the alternative activation pathway of the complement cascade. It regulates this pathway by targeting C3 convertase (C3bBb) for degradation and by acting as a co-factor in the cleavage and inactivation of C3b. Complement factor H is a soluble ~155 kDa glycoprotein present in serum at a relatively high concentration, averaging between 200 – 800 ug/ml. It is also expressed locally by many cells types including epithelial cells and platelets (Ferreira et al. 2010, Parente et al. 2017). Due to the major regulatory functionality of complement factor H, mutations and polymorphisms of the encoding gene are associated with inappropriate recognition of “self” cell markers in patients and consequently results in complement mediated tissue damage and disease. These mutations are associated with various diseases including atypical haemolytic uremic syndrome and C3 glomerulophathy (Martinez-Barricate et al. 2008, Cserhalmi et al. 2017). The purified Mouse anti Human complement factor H antibody, clone C06-2F3 (MCA6228GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human complement factor H antibody, clone C01-4A10 (MCA6229B) as the detection antibody. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified native human complement factor H
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- ELISA Matched Pair(s)
-
ELISA Matched Pair-Detection Antibody:
MCA6229B
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
MCA6228GA
100005532
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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