"A question of Life or Death" - differentiating between healthy and apoptotic cells
Dead or Alive on the ZE5™ - Using flow cytometry to measure apoptosis
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No experiment is complete without all the pieces and one key piece is confidence in the health of your cells. Determining the health of your cells is a crucial component to ensure the reproducibility of your experiments and it can even be the experimental result. Whether you are determining the viability of cells in culture, proliferation responses, understanding whether treatments cause apoptosis or autophagy, or simply wanting to improve your Flow Cytometry data, our years of experience providing reliable products to fit into many applications will give you the confidence you need to get the right result.
Determining cell viability is vital in many cellular assays. It can be used as the main experimental data in toxicity assays, to determine side effects of therapeutics or experimental conditions, to provide accurate information on cellular activity, or to simply ensure you are studying a live population. We have many reagents available, in easy-to-use formulations for determining cell viability. This includes both fixable and non-fixable dyes for use in Flow Cytometry and Microscopy.
The increase or decrease in cell growth and division can be the key to cytotoxicity and apoptosis assays and can be used to determine the effect of experimental treatment on cellular replication.
Apoptosis is the process of programed cell death characterized by distinct morphological and biochemical changes within a cell. It is crucial to regulate immune responses, development and can be impaired in cancer. We have easy-to-use apoptosis kits, annexin V reagents and antibodies against common apoptosis markers to determine cellular apoptosis levels.
If you are interested in our specialist apoptosis related products, such as cell surface death receptor signaling, cytoplasmic proteins involved in signaling, or the role of mitochondria in apoptosis. See our full range of apoptosis products.
Autophagy is the orderly process of degradation and recycling of cytoplasmic components though the lysosome. The induction of autophagy can be through starvation or growth factor suppression, ER stress, infection or immune signals. Autophagy is thought to be a repair and survival response during stress and infection and has been linked closely to apoptosis.
If you are interested in antibodies against upstream events which can lead to autophagy or those involved in macroautophagy including antibodies against ATG and ATG related proteins see our full range of autophagy products.