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TNF Alpha antibody

Rabbit anti Bovine TNF Alpha:Biotin

Product Type
Polyclonal Antibody
Polyclonal IgG
TNF Alpha

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 50 µg loader
List Price Your Price

Rabbit anti Bovine TNF alpha antibody recognizes bovine tumour necrosis factor alpha (TNF alpha).

TNF alpha is produced by a range of cells including macrophages, monocytes, T and B cells, neutrophils and endothelial cells. TNF alpha has a key regulatory role in the bovine immune response, where it is involved in modulating the expression of numerous cytokines, growth factors, inflammatory mediators and acute phase proteins.

Target Species
Product Form
Purified IgG conjugated to Biotin - liquid
Antiserum Preparation
Antisera to bovine TNF-alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Recombinant bovine tumour necrosis factor alpha (TNF-α)
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml
For research purposes only
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.05 ug/ml 0.5 ug/ml
Western Blotting 0.1 ug/ml 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
This product may be used as a detection antibody in a sandwich ELISA together with AHP2383 as the capture reagent. Recombinant bovine TNF-alpha (PBP035) may be used as a standard.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Bovine TNF Alpha AHP2383 E WB 0.1 mg loader
List Price Your Price
Description Rabbit anti Bovine TNF Alpha
Recombinant Bovine TNF Alpha PBP035 E 5 µg loader
List Price Your Price
Description Recombinant Bovine TNF Alpha

References for TNF Alpha antibody

  1. Rebollada-Merino, A. et al. (2023) Immunopathology of early and advanced epididymis lesions caused by Brucella ovis in rams.
    Vet Immunol Immunopathol. 261: 110621.

Entrez Gene
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space


139047 151210

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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