EGF antibody
Rabbit anti Human EGF
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- EGF
Antibody Quality and Performance Guarantee
| Rabbit anti Human EGF recognizes human Epidermal Growth Factor, a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, expressed by a variety of cells. The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the ~170 kDa EGF receptor (EGFR), which also binds other EGF-like ligands with high affinity, including TGF-a (transforming growth factor alpha), VGF (vaccinia virus growth factor) and Betacellulin, expressed in the pancreas, small intestine and by certain tumour cells (Seno et al. 1996). Receptor activation through EGF binding triggers several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water.
For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add azide.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Antiserum Preparation
- Antisera to human EGF were raised by repeated immunisations of rabbits with highly purified antigen.
Purified IgG prepared by affinity chromatography. - Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present.
- Carrier Free
- Yes
- Immunogen
- Recombinant human EGF (PHP030A).
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays | |
<0.1ug/ml | |
| Immunohistology - Paraffin 1 | |
5.0ug/ml | |
| Western Blotting | |
0.1 | 0.2ug/ml |
- 1This product requires heat-mediated pre-treatment of paraffin sections prior to staining. Citrate buffer pH 6.0 is recommended for this purpose
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This purified human EGF antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human EGF antibody (AHP767B) as the detection reagent and recombinant human EGF (PHP030A) as the standard.
- Histology Positive Control Tissue
- Human kidney
- Western Blotting
- Rabbit anti human EGF may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human EGF (PHP030A) as the positive control.
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| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
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Further Reading
-
Carpenter, G. & Cohen, S. (1990) Epidermal growth factor.
J Biol Chem. 265 (14): 7709-12.
- RRID
- AB_2095966
- UniProt
- P01133
- Entrez Gene
- EGF
- GO Terms
- GO:0005886 plasma membrane
- GO:0006260 DNA replication
- GO:0005509 calcium ion binding
- GO:0001525 angiogenesis
- GO:0002576 platelet degranulation
- GO:0016021 integral to membrane
- GO:0005154 epidermal growth factor receptor binding
- GO:0030297 transmembrane receptor protein tyrosine kinase activator activity
- GO:0045741 positive regulation of epidermal growth factor receptor activity
- View More GO Terms
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0008083 growth factor activity
- GO:0030168 platelet activation
- GO:0031093 platelet alpha granule lumen
- GO:0035413 positive regulation of catenin import into nucleus
- GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
- GO:0043406 positive regulation of MAP kinase activity
- GO:0045840 positive regulation of mitosis
- GO:0051048 negative regulation of secretion
- GO:0090279 regulation of calcium ion import
- GO:2000008 regulation of protein localization at cell surface
View more products with EGF specificity
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