EGF antibody

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Rabbit anti Human EGF:Biotin

Rabbit anti Human EGF

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP767B
Datasheet
E WB
MSDS
50 µg loader

AHP767
Datasheet
E FN P* WB
MSDS
0.1 mg loader

Rabbit anti human EGF recognizes human Epidermal Growth Factor, a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, expressed by a variety of cells.

The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the ~170 kDa EGF receptor (EGFR), which also binds other EGF-like ligands with high affinity, including TGF-a (transforming growth factor alpha), VGF (vaccinia virus growth factor) and Betacellulin, expressed in the pancreas, small intestine and by certain tumour cells (Seno et al. 1996). Receptor activation through EGF binding triggers several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution
Reconstitute with 0.1ml distilled water.
For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add azide.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation
Antisera to human EGF were raised by repeated immunisations of rabbits with highly purified antigen.
Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present.
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Recombinant human EGF (PHP030A).
Approx. Protein Concentrations
IgG concentration 0.1mg/ml after reconstitution.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P01133
Entrez Gene
EGF
GO Terms
GO:0005886 plasma membrane
GO:0006260 DNA replication
GO:0005509 calcium ion binding
GO:0001525 angiogenesis
GO:0002576 platelet degranulation
GO:0016021 integral to membrane
GO:0005154 epidermal growth factor receptor binding
GO:0030297 transmembrane receptor protein tyrosine kinase activator activity
GO:0045741 positive regulation of epidermal growth factor receptor activity
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0008083 growth factor activity
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0035413 positive regulation of catenin import into nucleus
GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
GO:0043406 positive regulation of MAP kinase activity
GO:0045840 positive regulation of mitosis
GO:0051048 negative regulation of secretion
GO:0090279 regulation of calcium ion import
GO:2000008 regulation of protein localization at cell surface
Regulatory
For research purposes only

Applications of EGF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
ELISA 0.5 2.0ug/ml
Functional Assays <0.1ug/ml
Immunohistology - Paraffin 1 5.0ug/ml
Western Blotting 0.1 0.2ug/ml
  1. 1This product requires heat-mediated pre-treatment of paraffin sections prior to staining. Citrate buffer pH 6.0 is recommended for this purpose
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This biotinylated human EGF antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with a purified human EGF antibody (AHP767) as the capture reagent and recombinant human EGF (PHP030A) as the standard.
ELISA
This purified human EGF antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human EGF antibody (AHP767B) as the detection reagent and recombinant human EGF (PHP030A) as the standard.
Histology Positive Control Tissue
Human kidney
Western Blotting
Rabbit anti human EGF may be used as in Western Blotting applications under either reducing or non-reducing conditions with recombinant human EGF (PHP030A) as the positive control.
Western Blotting
Rabbit anti human EGF may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human EGF (PHP030A) as the positive control.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Carpenter, G. & Cohen, S. (1990) Epidermal growth factor.
    J Biol Chem. 265 (14): 7709-12.