WNT-3a antibody

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Rabbit anti Human WNT-3a:Biotin

Rabbit anti Human WNT-3a

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2188B E WB 50 µg
AHP2188 E P* WB 0.1 mg
Rabbit anti Human WNT-3a antibody recognizes human WNT-3a, a secreted protein and member of the Wnt family of signalling proteins, acting as a ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3a plays key roles in many developmental and cell fate decisions, including embryonic patterning, CNS development, and cytoskeletal formation.

Wnt-3a acts as a promoter of the proliferation of ventral midbrain dopaminergic neuron precursor cells, and has been implicated in the progressive differentiation of anterior neural cells, as well as regulating the survival and expansion of neural progenitors derived from human embryonic stem cells. Wnt3a acts upstream of the Notch signalling pathway, in the regulation of somitogenesis, controlled by a segmentation clock, consisting of molecular oscillators in the Notch, Wnt3a and Fgf8 pathways(Aulehla et al. 2003).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antiserum to human WNT-3a were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Immunogen
Recombinant human WNT-3a.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml after reconstitution
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

Please see label for expiry date.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P56704 Related reagents
Entrez Gene
WNT3A Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001756 somitogenesis
GO:0002092 positive regulation of receptor internalization
GO:0003136 negative regulation of heart induction by canonical Wnt receptor signaling pathway
GO:0004871 signal transducer activity
GO:0007223 Wnt receptor signaling pathway, calcium modulating pathway
GO:0005110 frizzled-2 binding
GO:0005201 extracellular matrix structural constituent
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0005769 early endosome
GO:0005770 late endosome
GO:0007267 cell-cell signaling
GO:0009880 embryonic pattern specification
GO:0007409 axonogenesis
GO:0008284 positive regulation of cell proliferation
GO:0009798 axis specification
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0021766 hippocampus development
GO:0021874 Wnt receptor signaling pathway involved in forebrain neuroblast division
GO:0021904 dorsal/ventral neural tube patterning
GO:0030198 extracellular matrix organization
GO:0030879 mammary gland development
GO:0030903 notochord development
GO:0030917 midbrain-hindbrain boundary development
GO:0032092 positive regulation of protein binding
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0035121 tail morphogenesis
GO:0035413 positive regulation of catenin import into nucleus
GO:0042472 inner ear morphogenesis
GO:0045121 membrane raft
GO:0045599 negative regulation of fat cell differentiation
GO:0048018 receptor agonist activity
GO:0048343 paraxial mesodermal cell fate commitment
GO:0060021 palate development
GO:0060026 convergent extension
GO:0061054 dermatome development
GO:0071300 cellular response to retinoic acid
GO:0071425 hemopoietic stem cell proliferation
GO:0090263 positive regulation of canonical Wnt receptor signaling pathway
Regulatory
For research purposes only

Applications of WNT-3a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
ELISA 0.5 2.0ug/ml
Immunohistology - Paraffin 1
Western Blotting 0.1 0.2ug/ml
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
AHP2188B may be used in a direct ELISA or as the detection antibody in a sandwich ELISA with (AHP2188) as the capture reagent.
ELISA
AHP2188 may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with (AHP2188B) as the detection antibody.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Lai, Y.H. et al. (2011) SOX4 interacts with plakoglobin in a Wnt3a-dependent manner in prostate cancer cells.
    BMC Cell Biol. 12: 50.
  2. Yan, L. et al. (2011) Activation of the canonical Wnt/β-catenin pathway in ATF3-induced mammary tumors.
    PLoS One. 6:e16515.