CXCL13 antibody

Rabbit anti Human CXCL13:Biotin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- CXCL13
Quick Links:
Rabbit anti Human CXCL13 antibody recognizes human BCA-1 (B cell attractant chemokine), a 10.1 kDa CXC cytokine designated CXCL13, a potent chemoattractant for B lymphocytes expressed at high levels in the liver and to a lesser extent by lymph nodes, spleen, stomach, appendix and salivary glands. BCA-1 functions as a ligand for Burkitt's lymphoma receptor 1 (BLR-1), a chemokine receptor designated CXCR5, expressed by all mature B lymphocytes. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - lyophilized
- Reconstitution
- Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
N.B. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antisera to human CXCL13 were raised by repeated immunisations of rabbits with highly purified antigen.
Purified IgG was prepared from whole serum by affinity chromatography. - Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human CXCL13.
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 6 months from date of despatch
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | ![]() |
0.25 | 1.0ug/ml |
Western Blotting | ![]() |
0.1 | 0.2ug/ml |
- ELISA
- This biotinylated human CXCL13 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human CXCL13 antibody (AHP766) as the capture reagent and recombinant human CXCL13 (PHP129) as the standard.
References for CXCL13 antibody
-
Gunn, M.D. et al. (1998) A B-cell-homing chemokine made in lymphoid follicles activates Burkitt’s lymphoma receptor-1.
Nature. 391: 799-803.
- RRID
- AB_2245651
- UniProt
- O43927
- Entrez Gene
- CXCL13
- GO Terms
- GO:0005615 extracellular space
- GO:0005625 soluble fraction
- GO:0006935 chemotaxis
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0007204 elevation of cytosolic calcium ion concentration
- GO:0007267 cell-cell signaling
- GO:0008009 chemokine activity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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