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CXCL13 antibody

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Rabbit anti Human CXCL13:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CXCL13

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AHP766B
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Rabbit anti Human CXCL13 antibody recognizes human BCA-1 (B cell attractant chemokine), a 10.1 kDa CXC cytokine designated CXCL13, a potent chemoattractant for B lymphocytes expressed at high levels in the liver and to a lesser extent by lymph nodes, spleen, stomach, appendix and salivary glands.

BCA-1 functions as a ligand for Burkitt's lymphoma receptor 1 (BLR-1), a chemokine receptor designated CXCR5, expressed by all mature B lymphocytes.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilized
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
N.B. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antisera to human CXCL13 were raised by repeated immunisations of rabbits with highly purified antigen.
Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This biotinylated human CXCL13 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human CXCL13 antibody (AHP766) as the capture reagent and recombinant human CXCL13 (PHP129) as the standard.

References for CXCL13 antibody

  1. Gunn, M.D. et al. (1998) A B-cell-homing chemokine made in lymphoid follicles activates Burkitt’s lymphoma receptor-1.
    Nature. 391: 799-803.

RRID
AB_2245651
UniProt
O43927
Entrez Gene
CXCL13
GO Terms
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0007267 cell-cell signaling
GO:0008009 chemokine activity
View more products with CXCL13 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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