IL-11 antibody | D08-4C12

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Mouse anti Human Interleukin-11

Product Type
Monoclonal Antibody
Clone
D08-4C12
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6204GA
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human interleukin-11 antibody, clone D08-4C12, recognizes interleukin-11 (IL-11), also known as AGIF. IL-11 belongs to the IL-6 family of cytokines, and plays a protective role in acute inflammatory settings (Traber et al. 2019). IL-11 has both pro- and anti-inflammatory functions through activation of signaling cascades (Lokau et al. 2017). IL-11 is largely absent in the body fluids of healthy people, but is upregulated in a series of diseases. IL-11 can be expressed in fibroblasts, and its inhibition has been suggested as a treatment for fibrotic disease (Schafer et al. 2017), including fibrotic cardiovascular diseases (Ye et al. 2019). A role of IL-11 has also been identified in development of inflammation-associated cancers, and its inhibition may be a therapeutic goal (Putoczki et al. 2013).

The purified Mouse anti Human interleukin-11 antibody, clone D08-4C12 (MCA6204GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human interleukin-11 antibody, clone C16-4B8 (MCA6205B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
E. coli derived recombinant human full length interleukin-11 (Pro22-Leu199)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P20809
Entrez Gene
IL11
GO Terms
GO:0005125 cytokine activity
GO:0005142 interleukin-11 receptor binding
GO:0005615 extracellular space
GO:0007267 cell-cell signaling
GO:0008083 growth factor activity
GO:0008284 positive regulation of cell proliferation
GO:0030168 platelet activation
GO:0030183 B cell differentiation
GO:0030219 megakaryocyte differentiation
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0043410 positive regulation of MAPKKK cascade
GO:0045444 fat cell differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046888 negative regulation of hormone secretion
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
Regulatory
For research purposes only

Applications of IL-11 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human Interleukin-11:Biotin MCA6205B E 0.1 mg loader

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