Antibody Conjugation Kits

Conjugation kits offer a convenient way of adding a label to an antibody. The label required will vary according to your application and experimental set up. Bio-Rad labeling kits offer a range of labels to match these varied experimental conditions such as StarBright Dyes and other fluorescent dyes, enzymes, and biotin/streptavidin.

Label Range at a Glance

The table below shows the labels available from Bio-Rad’s range of labeling kits. For more details please refer to the information below the table.

 Flow; Flow Cytometry, WB; Western Blot, IHC: immunohistochemistry, IF: immunofluorescence

TrailBlazer Tag and TrailBlazer StarBright Dye Label Kits 

TrailBlazer^TM Tag and StarBright^TM Dye Label Kits use the innovative technology of SpyTag, SpyCatcher and StarBright Dyes that allows you to generate your own high quality conjugated antibodies. Select from the range of StarBright Dyes to suit your flow assays and western blots best.

In flow, StarBright Dyes have been designed to be stable with superior brightness, narrow excitation and emission characteristics, improving resolution of specific cell populations and minimizing spillover and spreading. In fluorescent western blot, you can expect bright, highly sensitive fluorescent detection with short exposure times.

Discover all the benefits of these StarBright Dye conjugation kits for yourself.

LYNX Kits

Fig.1. Peripheral blood lymphocytes were stained with CD3 (MCA463) conjugated to ReadiLink 700/713 Antibody Labeling Kit (1351008) to detect T cells. Data acquired on the ZE5 Cell Analyzer.

LYNX Rapid and Rapid Plus Conjugation Kits are available in a wide range of options including common fluorophores, enzymes, and biotin/streptavidin conjugations, suitable for use in flow cytometry, ELISA, western blotting, immunohistochemistry, and immunofluorescence. Identical protocols for small and large scale (up to milligram quantities of antibody) conjugations allow for fast one-step conjugation of proteins and antibodies using a method that is superior to traditional kits and techniques. In contrast to conventional conjugation, LYNX Conjugation Kits offer consistent results with no post-conjugation clean-up process required; therefore, the antibodies are ready to use immediately.

Conjugate your antibodies in 3 easy steps with only 30 seconds hands-on time and no purification required. The total conjugation time is fifteen minutes with the LYNX Rapid Plus Conjugation Kits or 3 hours with the LYNX Rapid Conjugation Kits, however the reaction may run overnight without any adverse effects.

ReadiLink Kits

ReadiLink Antibody Conjugation Kits offer ten unique fluorescence conjugation options for small volumes. Quickly label your antibody of interest in two easy steps in just over an hour with the unique chemistry of ReadiLink Dyes. The excitation wavelengths and narrow emissions spectra of the fluorophores ranging from violet to far red are ideal for multicolor flow cytometry. The infrared excitable conjugations are suitable for westerns, ELISA, and in-vivo imaging.

Degree of Labeling (DOL) Calculation

Want to know how much fluorophore is conjugated to your antibody?