CXCL13 antibody
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Rabbit anti Human CXCL13 antibody recognizes human BCA-1 (B cell attractant chemokine), a 10.1 kDa CXC cytokine designated CXCL13, a potent chemoattractant for B lymphocytes expressed at high levels in the liver and to a lesser extent by lymph nodes, spleen, stomach, appendix and salivary glands.
BCA-1 functions as a ligand for Burkitt's lymphoma receptor 1 (BLR-1), a chemokine receptor designated CXCR5, expressed by all mature B lymphocytes.
BCA-1 functions as a ligand for Burkitt's lymphoma receptor 1 (BLR-1), a chemokine receptor designated CXCR5, expressed by all mature B lymphocytes.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - lyophilised
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
N.B. For long term storage the addition of 0.09% sodium azide is recommended. - Reconstitution
- Reconstitute with 0.1ml distilled water.
For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antisera to human CXCL13 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- None present.
- Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Recombinant human CXCL13.
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 6 months from date of despatch
More Information
- UniProt
- O43927
- Entrez Gene
- CXCL13
- GO Terms
- GO:0005615 extracellular space
- GO:0005625 soluble fraction
- GO:0006935 chemotaxis
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0007204 elevation of cytosolic calcium ion concentration
- GO:0007267 cell-cell signaling
- GO:0008009 chemokine activity
- Regulatory
- For research purposes only
Applications of CXCL13 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.25 | 1.0ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml | |
| ELISA | 0.5ug/ml | ||
| Western Blotting | 0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This biotinylated human CXCL13 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human CXCL13 antibody (AHP766) as the capture reagent and recombinant human CXCL13 (PHP129) as the standard.
- ELISA
- This purified human CXCL13 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human CXCL13 antibody (AHP766B) as the detection reagent and recombinant human CXCL13 (PHP129) as the standard.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for CXCL13 antibody
-
Gunn, M.D. et al. (1998) A B-cell-homing chemokine made in lymphoid follicles activates Burkitt’s lymphoma receptor-1.
Nature. 391: 799-803.
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