CXCL13 antibody

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Rabbit anti Human CXCL13:Biotin

Rabbit anti Human CXCL13

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP766B E WB 50 µg
AHP766 E WB 0.1 mg
Rabbit anti Human CXCL13 antibody recognizes human BCA-1 (B cell attractant chemokine), a 10.1 kDa CXC cytokine designated CXCL13, a potent chemoattractant for B lymphocytes expressed at high levels in the liver and to a lesser extent by lymph nodes, spleen, stomach, appendix and salivary glands.

BCA-1 functions as a ligand for Burkitt's lymphoma receptor 1 (BLR-1), a chemokine receptor designated CXCR5, expressed by all mature B lymphocytes.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
N.B. For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution
Reconstitute with 0.1ml distilled water.
For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antisera to human CXCL13 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present.
Immunogen
Recombinant human CXCL13.
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
O43927 Related reagents
Entrez Gene
CXCL13 Related reagents
GO Terms
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0007267 cell-cell signaling
GO:0008009 chemokine activity
Regulatory
For research purposes only

Applications of CXCL13 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
ELISA 0.5ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This biotinylated human CXCL13 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human CXCL13 antibody (AHP766) as the capture reagent and recombinant human CXCL13 (PHP129) as the standard.
ELISA
This purified human CXCL13 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human CXCL13 antibody (AHP766B) as the detection reagent and recombinant human CXCL13 (PHP129) as the standard.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for CXCL13 antibody

  1. Gunn, M.D. et al. (1998) A B-cell-homing chemokine made in lymphoid follicles activates Burkitt’s lymphoma receptor-1.
    Nature. 391: 799-803.