IL-5 antibody

Rabbit anti Human Interleukin-5

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-5

Product Code Applications Pack Size List Price Your Price Qty
AHP1034
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F FN P WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rabbit anti Human Interleukin-5 antibody recognizes human IL-5 (Interleukin-5), a disulphide-linked homodimer consisting of two 115 amino acid chains, which is homologous to mouse eosinophil differentiation factor (EDF) and is produced by mast cells, eosinophils and T cells.

IL-5 is a lineage-specific cytokine involved in the development and stimulation of eosinophils, and has been linked with diseases involving abnormally high levels of eosinophils, including parasitic infections and allergic airways eosinophilia, the major pathological symptom of allergic asthma.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation
Antisera to human IL-5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human IL-5.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
6 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Flow Cytometry
Functional Assays 0.05 0.09ug/ml
Immunohistology - Paraffin
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This antibody may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with AHP1034B as the detection antibody

Description Product Code Applications Pack Size List Price Your Price Quantity
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:FITC
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:RPE
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):Biotin
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

Further Reading

  1. Campbell, H.D. et al. (1987) Molecular cloning, nucleotide sequence, and expression of the gene encoding human eosinophil differentiation factor (interleukin 5).
    Proc Natl Acad Sci U S A. 84 (19): 6629-33.
  2. Wang, J. et al. (1998) Circulating, but not local lung, IL-5 is required for the development of antigen-induced airways eosinophilia.
    J Clin Invest. 102 (6): 1132-41.

RRID
AB_2127327
UniProt
P05113
Entrez Gene
IL5
GO Terms
GO:0005125 cytokine activity
GO:0005137 interleukin-5 receptor binding
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
View more products with IL-5 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products