IL-22 antibody | AbD20463
IL-22 is a T and NK cell cytokine, a member of IL-10 cytokine family and is expressed by mitogen activated bovine peripheral blood γδ T cells. IL-22 is rapidly produced by splenic cells and can be produced by Th17 cells. IL-22 takes part in regulating the immune responses of adaptive and innate immune systems.
Human anti Bovine Interleukin-22, clone AbD20463 has been successfully used to analyze IL-22 expression in PBMC from M. bovis. – infected cattle. Bovine tuberculin induced IL-22 and IL-17A production in M. bovis. infected cattle was observed in both CD4+ T cells and γδ T cell populations (Steinbach et al. 2016).
- Target Species
- Product Form
- A bivalent human recombinant Fab antibody selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Fc-fusion protein containing the amino acid sequence 34-190 from bovine interleukin-22
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
His-tag is a registered trademark of EMD Biosciences.
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- For research purposes only
Applications of IL-22 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
Useful Reagents Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Rat anti DYKDDDDK Tag:HRP||MCA4764P||E P WB||0.1 mg|
Product Specific References
Antibody Characterization Reference
Steinbach, S. et al. (2016) CD4+ and γδ T Cells are the main Producers of IL-22 and IL-17A in Lymphocytes from Mycobacterium bovis-infected Cattle.
Sci Rep. 6: 29990.
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