Haptoglobin antibody | E07-10E9
Mouse anti Human Haptoglobin
- Product Type
- Monoclonal Antibody
- Clone
- E07-10E9
- Isotype
- IgG1
- Specificity
- Haptoglobin
| Mouse anti Human haptoglobin antibody, clone E07-10E9, recognizes haptoglobin, an alpha sialoglycoprotein. Synthesis of haptoglobin is induced by IL-6, IL-1 beta and TNF and it is produced as a pro-protein before it is cleaved in the endoplasmic reticulum by C1r-like protein, to generate the mature form (Redmond et al. 2018). Haptoglobin is composed of two peptide chains referred to as the alpha and beta chains. It is largely expressed by hepatocytes, but is also expressed to a lesser extent in lung, skin and kidney tissues (Vanuytsel et al. 2013). A key role of haptoglobin is its hemoglobin binding capacity, which is utilized during hemolysis to prevent iron loss and subsequent kidney damage. The haptoglobin-hemoglobin complex is removed by the CD163 receptor expressed on the surface of monocytes and macrophages (Sadrzadeh & Bozorgmehr 2004). Haptoglobin also plays roles in the innate and adaptive immune system and has anti-inflammatory activity such as inhibiting monocyte production of TNF, IL-11 and IL-12 (Arredouani et al. 2005). The purified Mouse anti Human haptoglobin antibody, clone E07-10E9 (MCA6234GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human haptoglobin antibody, clone C08-1E10 (MCA6235B) as the detection antibody. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Native haptoglobin purified from human plasma
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- ELISA Matched Pair(s)
-
ELISA Matched Pair-Detection Antibody:
MCA6235B
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- UniProt
- P00738
- Entrez Gene
- HP
- GO Terms
- GO:0006952 defense response
- GO:0004252 serine-type endopeptidase activity
- GO:0005576 extracellular region
- GO:0006508 proteolysis
- GO:0006879 cellular iron ion homeostasis
- GO:0010942 positive regulation of cell death
- GO:0030492 hemoglobin binding
- GO:0051354 negative regulation of oxidoreductase activity
- GO:2000296 negative regulation of hydrogen peroxide catabolic process
- View More GO Terms
MCA6234GA
100005291
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with HAPTOGLOBIN specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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