Protocols, application guides, tips and tricks
At Bio-Rad we aim to provide you with a reliable source of antibodies and support you with the design and optimization of your experiments. To help you with your experiments, we have developed a range of useful application specific guides, protocols and tips for:
To view all recommended protocols, please visit bio-rad-antibodies.com/protocols.
You can access our antibody resources by clicking on the relevant sections below.
Measuring DNA content and cell cycle in flow cytometry can give you valuable information on specific cell populations and can be measured by staining cells with DNA binding dyes, such as propidium iodide or 7-AAD.
Absorbance charts for our fluorochrome dyes showing both our single and tandem dyes.
Tips to help you with your Immunohistochemistry experiments.
PrecisionAb™ Lab-Validated Antibodies for Western Blotting
These antibodies are fully lab-validated using strict testing criteria to deliver superior performance in western blotting detection.
Bio-Rad exclusively offers two on-line calculators for alamarBlue® generated colorimetric and fluorometric readings. Your raw data can be converted into quantitative results and exported to Excel with a few clicks.
Useful resources which include an "Introduction to Flow Cytometry" guide, protocols and tips to help you design multi-color Flow Cytometry panels.
Our "Introduction to Flow Cytometry" guide is aimed at beginners, and covers the principles of fluorescence, data analysis advice and troubleshooting.
Discover a selection of Flow Cytometry protocols for direct and indirect staining of cells and blood. We also provide several protocols for intracellular stainings.
Ten tips and tricks to help you with the design of multi-color Flow Cytometry panels. From choosing the brightest fluorophores to using tandem dyes when designing panels of eight or more colors.
Tips for the optimization of intracellular Flow Cytometry staining results using Leucoperm™.
An overview of what an isotype control is, when and how to use it and what additional controls to perform in your Flow Cytometry experiments.
Detailed information about the excitation and emission maxima of all fluorophores available from Bio-Rad.
These resources include an "Introduction to Western Blotting" guide, protocols and an easy to follow Western Blotting video protocol.
A useful Western Blotting guide covering the core process, troubleshooting, and quantification steps. A great resource to gain a quick overview of this popular laboratory technique.
Learn how to perform the different Western Blotting steps with our protocols and hands-on video showing SDS-PAGE separation of proteins.
An overview of what a loading control is, why they are crucial and how to select the right loading controls for your Western Blotting experiments.
These resources include an "Introduction to ELISA" guide, protocols, tips, troubleshooting and advice.
Our "Introduction to ELISA" guide includes basic ELISA procedures, different types of ELISA, detection, results and sensitivity.
Protocols for sandwich ELISAs, direct, indirect and competition ELISAs.
A selection of helpful ELISA tips which include, setting up your ELISA, consistency between wells, advice on buffers, antibodies, coating, samples, blocking and washing.
A table of typical ELISA related problems, showing the possible causes, and solutions to achieve successful results.
These resources include protocols and information about our fluorophore offering.
View our Immunofluorescence (IF) protocols for cell suspensions.
A selection of helpful IF tips which include, microscope compatibility, and fluorophore selection based on quantum yields and photostability.
These include protocols, and tips for IHC staining optimization.
A selection of protocols for performing various Immunohistochemistry techniques ranging from IHC-paraffin to IHC-frozen.
Tips to help you with the optimization of your Immunohistochemistry experiments.
Crucial controls to help you optimize your IHC experiments.
A webinar overview of the different IHC protocol steps, the most common pitfalls and hands-on tips on how to troubleshoot this complex type of experiment.
View our Immunoprecipitation resources.
Immunoprecipitation with SureBeads™ Protein A/G Magnetic Beads.
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