CCL2 antibody | AA03-4H10

Mouse anti Human CCL2:Biotin

Product Type
Monoclonal Antibody
Clone
AA03-4H10
Isotype
IgG1
Specificity
CCL2

Product Code Applications Pack Size List Price Your Price Qty
MCA6219B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader
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Mouse anti Human CCL2 antibody, clone AA03-4H10, recognizes C-C motif chemokine 2, also known as MCP1, HC11, and MCAF. CCL2 is a constitutively expressed chemokine, which is upregulated during the inflammatory response and promotes recruitment of immune cells to specific sites. Many cell types can produce CCL2, including endothelial, epithelial, myeloid, smooth muscle cells and fibroblasts, and CCL2 is able to recruit monocytes, basophils, T lymphocytes and NK cells (Lim et al. 2016). CCL2 promotes cancer development and metastasis through binding to the cognate receptor CCR2, and CCL2-CCR2 signaling has multiple tumorigenic roles. The tumor microenvironment itself produces CCL2, which facilitates the accumulation of tumor associated macrophages (TAMs), which have immune suppressive and tumor promoting functions (Yoshimura et al. 2018).

The biotinylated Mouse anti Human CCL2 antibody, clone AA03-4H10 (MCA6219B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human CCL2 antibody, clone AA07-3B8 (MCA6218GA) as the capture antibody.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human CCL2 (Gln24-Thr99)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human CCL2 MCA6218GA E 0.1 mg loader
List Price Your Price
loader
Description Mouse anti Human CCL2

Synonyms
MCP-1
UniProt
P13500
Entrez Gene
CCL2
GO Terms
GO:0002548 monocyte chemotaxis
GO:0007155 cell adhesion
GO:0004672 protein kinase activity
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0006468 protein phosphorylation
GO:0006874 cellular calcium ion homeostasis
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0006959 humoral immune response
GO:0007187 G-protein signaling, coupled to cyclic nucleotide second messenger
GO:0007259 JAK-STAT cascade
GO:0008009 chemokine activity
GO:0019079 viral genome replication
GO:0031727 CCR2 chemokine receptor binding
GO:0048246 macrophage chemotaxis
View more products with CCL2 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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