IL-12 p40 antibody | D14-5G11
Mouse anti Human Interleukin-12 p40
- Product Type
- Monoclonal Antibody
- Clone
- D14-5G11
- Isotype
- IgG1
- Specificity
- IL-12 p40
Mouse anti Human Interleukin-12 p40 antibody, clone D14-5G11, recognizes interleukin-12 p40 (IL-12 p40) also known as interleukin-12 subunit beta and cytotoxic lymphocyte maturation factor 40 kDa subunit. IL-12 is a heterodimeric cytokine composed of 2 subunits, a 35 kDa subunit (p35) and a 40 kDa subunit (p40). The IL-12 p40 subunit is largely expressed by dendritic cells, monocytes, macrophages, neutrophils, B-lymphocytes and keratinocytes (Airoldi et al. 2002). It is able to form a homodimer, which was believed to be inactive. However it has subsequently been demonstrated to have proinflammatory properties through its induction of inducible nitric oxide synthase in microglia (Pahan et al. 2001, Jana et al. 2009).
IL-12 p40 coupled to the protein p19 forms the heterodimer IL-23 (p19-p40), when this formation takes place in activated dentritic cells the p19-p40 binds to its receptor IL-12Rβ1 to activate the STAT4 signaling pathway. This results in the stimulation of IFNγ production along with proliferation of PHA blast T cells (Airoldi et al. 2002). The purified Mouse anti Human interleukin-12 p40 antibody, clone D14-5G11 (MCA6194GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human interleukin-12 p40 antibody, clone M18 (MCA6195B) as the detection antibody. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- E. coli derived recombinant human full length interleukin-12 p40 (Ile23-Ser328)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- ELISA Matched Pair(s)
- ELISA Matched Pair-Detection Antibody: MCA6195B
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1.0 ug/ml |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse anti Human Interleukin-12 p40:Biotin | MCA6195B | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse anti Human Interleukin-12 p40:Biotin |
- Synonyms
- IL-12B
- UniProt
- P29460
- Entrez Gene
- IL12B
- GO Terms
- GO:0001916 positive regulation of T cell mediated cytotoxicity
- GO:0002230 positive regulation of defense response to virus by host
- GO:0002827 positive regulation of T-helper 1 type immune response
- GO:0002860 positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
- GO:0016020 membrane
- GO:0004896 cytokine receptor activity
- GO:0005125 cytokine activity
- GO:0005143 interleukin-12 receptor binding
- GO:0007050 cell cycle arrest
- View More GO Terms
- GO:0050829 defense response to Gram-negative bacterium
- GO:0010224 response to UV-B
- GO:0010535 positive regulation of activation of JAK2 kinase activity
- GO:0016477 cell migration
- GO:0019953 sexual reproduction
- GO:0032693 negative regulation of interleukin-10 production
- GO:0032700 negative regulation of interleukin-17 production
- GO:0032725 positive regulation of granulocyte macrophage colony-stimulating factor production
- GO:0032729 positive regulation of interferon-gamma production
- GO:0032733 positive regulation of interleukin-10 production
- GO:0032735 positive regulation of interleukin-12 production
- GO:0032740 positive regulation of interleukin-17 production
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0032819 positive regulation of natural killer cell proliferation
- GO:0034105 positive regulation of tissue remodeling
- GO:0034393 positive regulation of smooth muscle cell apoptosis
- GO:0042088 T-helper 1 type immune response
- GO:0042093 T-helper cell differentiation
- GO:0042095 interferon-gamma biosynthetic process
- GO:0042104 positive regulation of activated T cell proliferation
- GO:0042346 positive regulation of NF-kappaB import into nucleus
- GO:0042510 regulation of tyrosine phosphorylation of Stat1 protein
- GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
- GO:0042520 positive regulation of tyrosine phosphorylation of Stat4 protein
- GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
- GO:0043382 positive regulation of memory T cell differentiation
- GO:0043514 interleukin-12 complex
- GO:0045078 positive regulation of interferon-gamma biosynthetic process
- GO:0045672 positive regulation of osteoclast differentiation
- GO:0045785 positive regulation of cell adhesion
- GO:0046982 protein heterodimerization activity
- GO:0048662 negative regulation of smooth muscle cell proliferation
- GO:0050729 positive regulation of inflammatory response
- GO:0051135 positive regulation of NK T cell activation
- GO:0051142 positive regulation of NK T cell proliferation
- GO:0070743 interleukin-23 complex
MCA6194GA
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