VEGF antibody
Rabbit anti Mouse VEGF
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- VEGF
Antibody Quality and Performance Guarantee
| Rabbit anti Mouse VEGF antibody recognizes mouse Vascular Endothelial Growth Factor (VEGF), a protein active in angiogenesis, vasculogenesis and endothelial cell growth. Rabbit anti Mouse VEGF antibody has not been cross adsorbed and may react with homologous proteins from related species. |
- Target Species
- Mouse
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to mouse VEGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant mouse VEGF
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5ug/ml | |
| Functional Assays | |
1.0ug/ml | 2.0ug/ml |
| Immunofluorescence | |
2.5ug/ml | |
| Immunohistology - Paraffin 1 | |
2.5ug/ml | |
| Western Blotting | |
0.1ug/ml | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA buffer pH 8.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for VEGF antibody
-
Nahrendorf, M. et al. (2007) The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions.
J Exp Med. 204: 3037-47. -
Lin, E.Y. et al. (2007) Vascular endothelial growth factor restores delayed tumor progression in tumors depleted of macrophages.
Mol Oncol. 1: 288-302. -
Araújo, W.F. et al. (2015) Renin-angiotensin system (RAS) blockade attenuates growth and metastatic potential of renal cell carcinoma in mice.
Urol Oncol. 33 (9): 389.e1-7. -
HogenEsch, H. et al. (2016) Angiogenesis in the skin of SHARPIN-deficient mice with chronic proliferative dermatitis.
Exp Mol Pathol. 101 (3): 303-307.
- Synonyms
- VPF
- RRID
- AB_2288247
- UniProt
- Q00731
- Entrez Gene
- Vegfa
- GO Terms
- GO:0005886 plasma membrane
- GO:0001541 ovarian follicle development
- GO:0001569 patterning of blood vessels
- GO:0001701 in utero embryonic development
- GO:0001822 kidney development
- GO:0001938 positive regulation of endothelial cell proliferation
- GO:0002053 positive regulation of mesenchymal cell proliferation
- GO:0003007 heart morphogenesis
- GO:0005615 extracellular space
- View More GO Terms
- GO:0006916 anti-apoptosis
- GO:0040007 growth
- GO:0007498 mesoderm development
- GO:0007595 lactation
- GO:0007610 behavior
- GO:0008083 growth factor activity
- GO:0008201 heparin binding
- GO:0008283 cell proliferation
- GO:0030855 epithelial cell differentiation
- GO:0031077 post-embryonic camera-type eye development
- GO:0042462 eye photoreceptor cell development
- GO:0043117 positive regulation of vascular permeability
- GO:0043129 surfactant homeostasis
- GO:0048010 vascular endothelial growth factor receptor signaling pathway
- GO:0048469 cell maturation
- GO:0048593 camera-type eye morphogenesis
- GO:0048739 cardiac muscle fiber development
- GO:0048844 artery morphogenesis
- GO:0051781 positive regulation of cell division
- GO:0060319 primitive erythrocyte differentiation
- GO:0060749 mammary gland alveolus development
- GO:0090190 positive regulation of branching involved in ureteric bud morphogenesis
AAM51
147094
154739
164812
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