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VEGF antibody

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN IF P * WB 0.1 mg loader
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Rabbit anti Mouse VEGF antibody recognizes mouse Vascular Endothelial Growth Factor (VEGF), a protein active in angiogenesis, vasculogenesis and endothelial cell growth.

Rabbit anti Mouse VEGF antibody has not been cross adsorbed and may react with homologous proteins from related species.

Target Species
Product Form
Purified IgG - lyophilized
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to mouse VEGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution.
For research purposes only
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Functional Assays 1.0ug/ml 2.0ug/ml
Immunofluorescence 2.5ug/ml
Immunohistology - Paraffin 1 2.5ug/ml
Western Blotting 0.1ug/ml 0.2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA buffer pH 8.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
This antibody may be used in indirect ELISA or as a capture reagent in a sandwich ELISA with AAM51B as a detection reagent and PMP67 as the standard.
Histology Positive Control Tissue

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
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Description Goat anti Rabbit IgG (Fc):Biotin
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
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Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
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Description Goat anti Rabbit IgG (Fc):HRP
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
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Description Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
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Description Sheep anti Rabbit IgG:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
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Description Antigen Retrieval Buffer, pH8.0
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB * 0.5 ml
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Description TidyBlot Western Blot Detection Reagent:HRP

References for VEGF antibody

  1. Nahrendorf, M. et al. (2007) The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions.
    J Exp Med. 204: 3037-47.
  2. Lin, E.Y. et al. (2007) Vascular endothelial growth factor restores delayed tumor progression in tumors depleted of macrophages.
    Mol Oncol. 1: 288-302.
  3. Araújo, W.F. et al. (2015) Renin-angiotensin system (RAS) blockade attenuates growth and metastatic potential of renal cell carcinoma in mice.
    Urol Oncol. 33 (9): 389.e1-7.
  4. HogenEsch, H. et al. (2016) Angiogenesis in the skin of SHARPIN-deficient mice with chronic proliferative dermatitis.
    Exp Mol Pathol. 101 (3): 303-307.

Flow Cytometry

Western Blotting

Entrez Gene
GO Terms
GO:0005886 plasma membrane
GO:0001541 ovarian follicle development
GO:0001569 patterning of blood vessels
GO:0001701 in utero embryonic development
GO:0001822 kidney development
GO:0001938 positive regulation of endothelial cell proliferation
GO:0002053 positive regulation of mesenchymal cell proliferation
GO:0003007 heart morphogenesis
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0040007 growth
GO:0007498 mesoderm development
GO:0007595 lactation
GO:0007610 behavior
GO:0008083 growth factor activity
GO:0008201 heparin binding
GO:0008283 cell proliferation
GO:0030855 epithelial cell differentiation
GO:0031077 post-embryonic camera-type eye development
GO:0042462 eye photoreceptor cell development
GO:0043117 positive regulation of vascular permeability
GO:0043129 surfactant homeostasis
GO:0048010 vascular endothelial growth factor receptor signaling pathway
GO:0048469 cell maturation
GO:0048593 camera-type eye morphogenesis
GO:0048739 cardiac muscle fiber development
GO:0048844 artery morphogenesis
GO:0051781 positive regulation of cell division
GO:0060319 primitive erythrocyte differentiation
GO:0060749 mammary gland alveolus development
GO:0090190 positive regulation of branching involved in ureteric bud morphogenesis


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