VEGF antibody

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Rabbit anti Mouse VEGF:Biotin

Rabbit anti Mouse VEGF

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAM51B E P WB 50 µg
AAM51 E FN IF P* WB 0.1 mg
Rabbit anti Mouse VEGF antibody recognizes mouse Vascular Endothelial Growth Factor (VEGF), a protein active in angiogenesis, vasculogenesis and endothelial cell growth.

Rabbit anti Mouse VEGF antibody has not been cross adsorbed and may react with homologous proteins from related species.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to mouse VEGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Immunogen
Recombinant mouse VEGF
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution.

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
3 months from date of reconstitution.

Please see label for expiry date.

More Information

UniProt
Q00731 Related reagents
Entrez Gene
Vegfa Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001541 ovarian follicle development
GO:0001569 patterning of blood vessels
GO:0001701 in utero embryonic development
GO:0001822 kidney development
GO:0001938 positive regulation of endothelial cell proliferation
GO:0002053 positive regulation of mesenchymal cell proliferation
GO:0003007 heart morphogenesis
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0040007 growth
GO:0007498 mesoderm development
GO:0007595 lactation
GO:0007610 behavior
GO:0008083 growth factor activity
GO:0008201 heparin binding
GO:0008283 cell proliferation
GO:0030855 epithelial cell differentiation
GO:0031077 post-embryonic camera-type eye development
GO:0042462 eye photoreceptor cell development
GO:0043117 positive regulation of vascular permeability
GO:0043129 surfactant homeostasis
GO:0048010 vascular endothelial growth factor receptor signaling pathway
GO:0048469 cell maturation
GO:0048593 camera-type eye morphogenesis
GO:0048739 cardiac muscle fiber development
GO:0048844 artery morphogenesis
GO:0051781 positive regulation of cell division
GO:0060319 primitive erythrocyte differentiation
GO:0060749 mammary gland alveolus development
GO:0090190 positive regulation of branching involved in ureteric bud morphogenesis
Regulatory
For research purposes only

Applications of VEGF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25ug/ml 1.0ug/ml
Immunohistology - Paraffin
Western Blotting 0.10ug/ml 0.20ug/ml
ELISA 0.5ug/ml
Functional Assays 1.0ug/ml 2.0ug/ml
Immunofluorescence 2.5ug/ml
Immunohistology - Paraffin 1 2.5ug/ml
Western Blotting 0.1ug/ml 0.2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA buffer pH 8.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This antibody may be used in direct ELISA or as a detection reagent in a sandwich ELISA with AAM51 as a capture reagent and PMP67 as the standard.
ELISA
This antibody may be used in indirect ELISA or as a capture reagent in a sandwich ELISA with AAM51B as a detection reagent and PMP67 as the standard.
Histology Positive Control Tissue
Kidney

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Application Based External Images

Flow Cytometry

Western Blotting

Product Specific References

References for VEGF antibody

  1. Nahrendorf, M. et al. (2007) The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions.
    J Exp Med. 204: 3037-47.
  2. Araújo, W.F. et al. (2015) Renin-angiotensin system (RAS) blockade attenuates growth and metastatic potential of renal cell carcinoma in mice.
    Urol Oncol. 33 (9): 389.e1-7.
  3. Lin, E.Y. et al. (2007) Vascular endothelial growth factor restores delayed tumor progression in tumors depleted of macrophages.
    Mol Oncol. 1: 288-302.
  4. HogenEsch H et al. (2016) Angiogenesis in the skin of SHARPIN-deficient mice with chronic proliferative dermatitis.
    Exp Mol Pathol. Oct 26. pii: S0014-4800(16)30084-3. [Epub ahead of print]
  5. Hogenesch, H. et al. (2016) Angiogenesis in the skin of SHARPIN-deficient mice with chronic proliferative dermatitis.
    Exp Mol Pathol. 101 (3): 303-7.