C-Reactive Protein antibody | F03-2C7

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Mouse anti Human C-Reactive Protein:Biotin

Product Type
Monoclonal Antibody
Clone
F03-2C7
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA6187B
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human C-reactive protein antibody, clone F03-2C7 recognizes, C-reactive protein (CRP). CRP is a member of the pentaxin family of proteins which target and activate various stages of the complement system. CRP is known to exist in 2 states, either as a monomer (mCRP) or as a pentamer (pCRP), where the pantamer is composed of 5 nearly identical subunits. The monomeric and pentameric forms have similar functions, but their relative abundance differs across various tissues. For example, pCRP is present in plasma, while mCRP is present mostly in the surface of damaged cells and platelets (Wu et al. 2015). In humans, levels of CRP increases 1,000-fold within 24 to 48 hours of infection, inflammation or tissue damage (Black et al. 2004).

In response to infection or tissue damage Interleukin-6, Interleukin-1 beta or glucocorticoids induce hepatocyte production of CRP. Available CRP can then detect foreign invading microorganisms, by binding carbohydrate structures. CRP is also able to bind modified low density lipoproteins and cells undergoing cell death, to assist with phagocytosis and clearance mechanisms. This is directly co-ordinated via CRP and other pentaxin proteins to prevent excessive tissue damage and clearance of any injured tissue components.

This biotinylated Mouse anti Human C-reactive protein antibody, clone F03-2C7 (MCA6187B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human C-reactive protein antibody, clone F01-1G4 (MCA6186GA) as the capture antibody.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length C-Reactive Protein (Phe17-Pro224)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P02741
Entrez Gene
CRP
GO Terms
GO:0005515 protein binding
GO:0006953 acute-phase response
GO:0046872 metal ion binding
GO:0008228 opsonization
GO:0010745 negative regulation of macrophage derived foam cell differentiation
GO:0010888 negative regulation of lipid storage
GO:0030169 low-density lipoprotein particle binding
GO:0033265 choline binding
GO:0051637 Gram-positive bacterial cell surface binding
Regulatory
For research purposes only

Applications of C-Reactive Protein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human C-Reactive Protein MCA6186GA E 0.1 mg loader

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