Secondary Antibody Selection Process - Step by Step
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- Select your secondary antibody
- Top tips
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Select your secondary antibody
Using the knowledge from the “Experimental design – how-to reference guide” section and your desired application, follow the steps below to discover your ideal secondary antibody.
Select:
- Target species
- Host
- Class and chain (found in the specificity filter)
- Isotype
- Format
- Check before you select – does your antibody need to be cross-adsorbed?
Use the filters to systemically select your requirements and discover the best secondary antibody for your experiment, then download the results.
Secondary Antibodies Range
| Description | Target | Host | Specificity | Format | Citations | Isotype | Code |
|---|
- When using more than one secondary antibody ensure that they don’t cross react
- When working with some immune tissues or cells that contain a lot of Fc receptors, it helps to choose a F(ab) or F(ab’)2 fragment to eliminate non-specific binding. Alternatively, block Fc receptors via an absorption step, using purified IgG from the host species of your secondary antibody or serum from target cells
- View our IHC tips when using a secondary antibody
Any questions? Let us help, contact our technical support team for advice
Optimize your Secondary Antibody Detection System Protocols and Resources