Secondary Antibody Selection Process - Step by Step

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Secondary Antibody Guide

Download or request a free printed copy of our new secondary antibody guide

Download or request a free printed copy of our new secondary antibody guide. It is easy-to-read and practical, explaining step-by-step what you need to know, what you need to do, and how to do it.

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Fundamentals of Secondary Antibodies

PODCASTS Fundamentals of Secondary Antibodies

Hear how you can get the right result with our series of secondary antibody podcasts

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Secondary Reagents in Flow Cytometry

Secondary Reagents in Flow Cytometry

Guiding the way to successful secondary detection

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Select your secondary antibody

Using the knowledge from the “Experimental design – how-to reference guide” section and your desired application, follow the steps below to discover your ideal secondary antibody.

Select:

  1. Target species
  2. Host
  3. Class and chain (found in the specificity filter)
  4. Isotype
  5. Format
  6. Check before you select – does your antibody need to be cross-adsorbed?

Use the filters to systemically select your requirements and discover the best secondary antibody for your experiment, then download the results.

Secondary Antibodies Range

    Description Target Host Clone Specificity Format Citations Isotype Applications Product Type Code

    Top tips

    • When using more than one secondary antibody ensure that they don’t cross react
    • When working with some immune tissues or cells that contain a lot of Fc receptors, it helps to choose a F(ab) or F(ab’)2 fragment to eliminate non-specific binding. Alternatively, block Fc receptors via an absorption step, using purified IgG from the host species of your secondary antibody or serum from target cells
    • View our IHC tips when using a secondary antibody

    Any questions? Let us help, contact our technical support team for advice


    Applications of Secondary AntibodiesProtocols and Resources
    Optimize your Secondary Antibody Detection System