SDF-1 Alpha antibody
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Goat anti Mouse SDF-1 Alpha
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- SDF-1 Alpha
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| Goat anti Mouse SDF-1 alpha antibody recognizes mouse SDF-1 alpha, otherwise known as CXCL12a, a stromal cell derived CXC chemokine which, together with SDF-1 beta, arises from alternative splicing of the SDF-1 gene, and acts as a chemoattractant for T and B lymphocytes, monocytes and migratory neurons. SDF-1 binds with high-affinity to the G protein–coupled receptor CXCR4 (fusin), and acts as an inhibitor of the CXCR4-mediated entry of HIV-1 virus into target T-cells. |
- Target Species
- Mouse
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antiserum to mouse SDF-1 alpha were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant mouse SDF-1 alpha expressed in E. coli (PMP77).
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 6 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays | |
2.5 | 3.5ug/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):HRP | ||||||
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):FITC | ||||||
- Synonyms
- CXCL12a
- RRID
- AB_2088306
- UniProt
- P40224
- Entrez Gene
- Cxcl12
- GO Terms
- GO:0001569 patterning of blood vessels
- GO:0001667 ameboidal cell migration
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0007281 germ cell development
- GO:0008009 chemokine activity
- GO:0008045 motor axon guidance
- GO:0008083 growth factor activity
- GO:0008354 germ cell migration
- View More GO Terms
- GO:0009897 external side of plasma membrane
- GO:0030335 positive regulation of cell migration
- GO:0042098 T cell proliferation
- GO:0050930 induction of positive chemotaxis
View more products with SDF-1 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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