IFN Gamma antibody | AbD00676

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Anti Human Interferon Gamma Antibody, clone AbD00676 gallery image 1 — Sandwich ELISA showing detection of human interferon gamma using **HCA043** as a capture reagent and HCA044 as a detection reagent.

Human anti Human Interferon Gamma

Product Type: Monoclonal Antibody
Clone: AbD00676
Isotype: HuCAL Fab bivalent

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
HCA043A	E*			50 µg
HCA043	E*			0.1 mg

Human anti Human interferon gamma antibody, clone AbD00676 recognizes human IFN gamma, a key cytokine in the immune response.

Product Details

Target Species: Human
Product Form: A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Preparation: Metal chelate affinity chromatography
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.01% Thiomersal
Immunogen: Purified recombinant human interferon gamma
Approx. Protein Concentrations: Antibody concentration 0.5 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life: 6 months from date of despatch

More Information

UniProt: P01579 Related reagents
Entrez Gene: IFNG Related reagents
GO Terms: GO:0000060 protein import into nucleus, translocation; GO:0002053 positive regulation of mesenchymal cell proliferation; GO:0003340 negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis; GO:0009615 response to virus; GO:0005125 cytokine activity; GO:0005133 interferon-gamma receptor binding; GO:0005615 extracellular space; GO:0006955 immune response; GO:0007050 cell cycle arrest; GO:0050796 regulation of insulin secretion; GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter; GO:0032735 positive regulation of interleukin-12 production; GO:0032747 positive regulation of interleukin-23 production; GO:0033141 positive regulation of peptidyl-serine phosphorylation of STAT protein; GO:0034393 positive regulation of smooth muscle cell apoptosis; GO:0042508 tyrosine phosphorylation of Stat1 protein; GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein; GO:0045429 positive regulation of nitric oxide biosynthetic process; GO:0045672 positive regulation of osteoclast differentiation; GO:0048662 negative regulation of smooth muscle cell proliferation; GO:0051044 positive regulation of membrane protein ectodomain proteolysis; GO:0051712 positive regulation of killing of cells of other organism; GO:0060333 interferon-gamma-mediated signaling pathway; GO:0060334 regulation of interferon-gamma-mediated signaling pathway; GO:0060550 positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity; GO:0060552 positive regulation of fructose 1,6-bisphosphate metabolic process; GO:0060557 positive regulation of vitamin D biosynthetic process; GO:0060559 positive regulation of calcidiol 1-monooxygenase activity; GO:0072308 negative regulation of metanephric nephron tubule epithelial cell differentiation
Acknowledgements: Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use: For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory: For research purposes only

Applications of IFN Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA ¹			1 ug/ml

¹ For use as a capture reagent in Sandwich ELISA we recommend the use of HISPEC ASSAY DILUENT for sample and antibody dilution.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA: HCA043 may be used in a sandwich ELISA assay as a capture antibody in combination with (HCA044) as detection antibody.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Human IgG F(ab')2:HRP	0500-0099	0.5 ml	E WB
Mouse anti c-Myc:HRP	MCA2200P	0.1 mg	C E P WB*
Mouse anti Penta Histidine Tag:HRP	MCA5995P	125 µl	E WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Hispec Assay Diluent	BUF049A	50 ml	E IY

Product Specific References

References for IFN Gamma antibody

Wienken, C.J. et al. (2010) Protein-binding assays in biological liquids using microscale thermophoresis.
Nat Commun. 1: 100.

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