IFN Gamma antibody | AbD00676
Human anti Human interferon gamma antibody, clone AbD00676 recognizes human IFN gamma, a key cytokine in the immune response.
Product Details
- Target Species
- Human
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Purified recombinant human interferon gamma
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 6 months from date of despatch
More Information
- UniProt
- P01579
- Entrez Gene
- IFNG
- GO Terms
- GO:0000060 protein import into nucleus, translocation
- GO:0002053 positive regulation of mesenchymal cell proliferation
- GO:0003340 negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
- GO:0009615 response to virus
- GO:0005125 cytokine activity
- GO:0005133 interferon-gamma receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0007050 cell cycle arrest
- GO:0050796 regulation of insulin secretion
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0032735 positive regulation of interleukin-12 production
- GO:0032747 positive regulation of interleukin-23 production
- GO:0033141 positive regulation of peptidyl-serine phosphorylation of STAT protein
- GO:0034393 positive regulation of smooth muscle cell apoptosis
- GO:0042508 tyrosine phosphorylation of Stat1 protein
- GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0045672 positive regulation of osteoclast differentiation
- GO:0048662 negative regulation of smooth muscle cell proliferation
- GO:0051044 positive regulation of membrane protein ectodomain proteolysis
- GO:0051712 positive regulation of killing of cells of other organism
- GO:0060333 interferon-gamma-mediated signaling pathway
- GO:0060334 regulation of interferon-gamma-mediated signaling pathway
- GO:0060550 positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
- GO:0060552 positive regulation of fructose 1,6-bisphosphate metabolic process
- GO:0060557 positive regulation of vitamin D biosynthetic process
- GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
- GO:0072308 negative regulation of metanephric nephron tubule epithelial cell differentiation
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of IFN Gamma antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA 1 | 1 ug/ml |
- 1 For use as a capture reagent in Sandwich ELISA we recommend the use of HISPEC ASSAY DILUENT for sample and antibody dilution.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | 0500-0099 | E WB | 0.5 ml |
|
|
| Mouse anti c-Myc:HRP | MCA2200P | C E P WB* | 0.1 mg |
|
|
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml |
Product Specific References
References for IFN Gamma antibody
-
Wienken, C.J. et al. (2010) Protein-binding assays in biological liquids using microscale thermophoresis.
Nat Commun. 1: 100.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
