CD120b antibody
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IF ResetRabbit anti Human CD120b:Biotin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- CD120b
Antibody Quality and Performance Guarantee
| Rabbit anti Human CD120b recognizes the soluble form of human CD120b, otherwise known as sTNF receptor type II (sTNFR1I), a member of the tumor necrosis factor receptor (TNFR) superfamily, produced by proteolytic cleavage of the membrane form by the metalloprotease TACE. CD120b plays a key role in regulating the inflammatory response, acting as a receptor for both tumour necrosis factor alpha (TNF-alpha) and TNF-beta, and is also involved in programmed cell death and anti-viral activity. sTNFR1I acts as an inhibitor of TNF-alpha induced responses, by acting as a decoy receptor. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - lyophilized
- Reconstitution
- Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to human CD120b were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human CD120b.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.25 | 1.0ug/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This biotinylated human CD120b antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with a purified human CD120b antibody (AHP1833) as the capture reagent.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
- Synonyms
- TNF-R2
- RRID
- AB_2206652
- UniProt
- P20333
- Entrez Gene
- TNFRSF1B
- GO Terms
- GO:0005886 plasma membrane
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0005031 tumor necrosis factor receptor activity
- GO:0045121 membrane raft
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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