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MIP-2 antibody

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Anti Mouse MIP-2 Antibody thumbnail image 1 Anti Mouse MIP-2 Antibody thumbnail image 2
Anti Mouse MIP-2 Antibody gallery image 1
Published customer image:
Rabbit anti Mouse MIP-2 antibody used for the identification of MIP-2 positive cells in mouse skin by immunohistochemistry on formailn fixed paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis of 12-O-tetradecanoylphorbol 13-acetate (TPA)-treated skin. TPA-treated skin from control (A–C) and afadin conditional knockout (CKO) (D–F) mice was stained with the antibodies shown at the top of each picture. Scale bar 50 μm.

From: Yoshida T, Iwata T, Takai Y, Birchmeier W, Yamato M, Okano T. Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice. Genes Cells. 2014 Nov;19(11):842-52.
Anti Mouse MIP-2 Antibody gallery image 2
Published customer image:
Rabbit anti Mouse MIP-2 antibody (AAM48) used to assess MIP-2 (CXCL2) expression in mouse hepatic tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
CXCL2 expression after bile duct ligation (BDL). B: CXCL2 immunohistochemical staining. CXCL2 protein was found to be expressed around areas of hepatocyte necrosis after BDL in wild-type mice. Original magnification: ×400. Original magnification: ×400.

From: Konishi T, Schuster RM, Goetzman HS, Caldwell CC, Lentsch AB.
Cell-specific regulatory effects of CXCR2 on cholestatic liver injury.
Am J Physiol Gastrointest Liver Physiol. 2019 Dec 1;317(6):G773-G783.
doi: 10.1152/ajpgi.00080.2019
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Rabbit anti Mouse MIP-2:Biotin

Rabbit anti Mouse MIP-2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AAM48B
Datasheet
C E P* WB
SDS
50 µg loader

AAM48
Datasheet
C E FN P* WB
SDS
0.1 mg loader

Rabbit anti Mouse MIP-2 antibody recognizes mouse macrophage inflammatory protein-2 (MIP-2), also known as CXCL2.

MIP-2 is expressed by macrophages and epidermal langerhans cells and is chemotactic for neutrophils.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - lyophilized
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to mouse MIP-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Recombinant mouse MIP-2
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution.

Storage Information

Storage
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
6 months from date of despatch
Guarantee
6 months from date of despatch

More Information

UniProt
P10889
Entrez Gene
Cxcl2
GO Terms
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008009 chemokine activity
Regulatory
For research purposes only

Applications of MIP-2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting 0.1 0.2ug/ml
ELISA 0.5ug/ml
Functional Assays 0.50ug/ml 0.90ug/ml
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting 0.1ug/ml 0.2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

    Sodium citrate buffer pH 6.0 is recommended for this purpose.
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

    Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Biotinylated Rabbit anti Mouse MIP-2 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with AAM48 as the capture antibody and PMP55 as the standard.
ELISA
Rabbit anti Mouse MIP-2 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with biotinylated Rabbit anti mouse MIP-2 antibody (AAM48B) as the detection antibody and recombinant Mouse MIP-2 protein (PMP55) as a standard.
Western Blotting
Biotinylated Rabbit anti Mouse MIP-2 antibody may used under either reducing or non-reducing conditions with PMP55 as the positive control.
Western Blotting
Rabbit anti Mouse MIP-2 antibody may be used under either reducing or non-reducing conditions with recombinant Mouse MIP-2 (PMP55) protein as a positive control.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Mouse MIP-2 AAM48 C E FN P* WB 0.1 mg loader
Rabbit anti Mouse MIP-2:Biotin AAM48B C E P* WB 50 µg loader
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
100x Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Positive Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Recombinant Mouse MIP-2 PMP55 E FN WB 20 µg loader
Recombinant Mouse MIP-2 PMP55 E FN WB 20 µg loader

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for MIP-2 antibody

  1. Tittel, A.P. et al. (2011) Kidney dendritic cells induce innate immunity against bacterial pyelonephritis.
    J Am Soc Nephrol. 22 (8): 1435-41.
  2. Roche, J.K. et al. (2007) CXCL1/KC and CXCL2/MIP-2 are critical effectors and potential targets for therapy of Escherichia coli O157:H7-associated renal inflammation.
    Am J Pathol. 170: 526-37.
  3. Di Carlo, E. et al. (2000) The combined action of IL-15 and IL-12 gene transfer can induce tumor cell rejection without T and NK cell involvement.
    J Immunol. 165: 3111-8.
  4. Cavallo, F. et al (2001) Interleukin 12-activated lymphocytes influence tumor genetic programs.
    Cancer Res. 61: 3518-23.
  5. González-López, A. et al. (2011) Inflammation and matrix remodeling during repair of ventilator-induced lung injury.
    Am J Physiol Lung Cell Mol Physiol. 301: L500-9.
  6. Lazic, M. et al. (2014) Differential regulation of inflammation and apoptosis in Fas-resistant hepatocyte-specific Bid-deficient mice.
    J Hepatol. 61: 107-15.
  7. Nicholas, J. et al. (2015) Time course of chemokine expression and leukocyte infiltration after acute skeletal muscle injury in mice.
    Innate Immun. 21 (3): 266-74.
  8. Yoshida, T. et al. (2014) Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.
    Genes Cells. 19: 842-52.
  9. Lasarte, S. et al. (2016) Sex Hormones Coordinate Neutrophil Immunity in the Vagina by Controlling Chemokine Gradients.
    J Infect Dis. 213 (3): 476-84.
  10. Salinas-Muñoz, L. et al. (2019) Estradiol impairs epithelial CXCL1 gradient in the cervix to delay neutrophil transepithelial migration during insemination.
    J Reprod Immunol. 132: 9-15.
  11. Konishi, T. et al. (2019) Cell-specific regulatory effects of CXCR2 on cholestatic liver injury.
    Am J Physiol Gastrointest Liver Physiol. 317 (6): G773-G783.

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