MIP-2 antibody
MIP-2 is expressed by macrophages and epidermal langerhans cells and is chemotactic for neutrophils.
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Biotin - lyophilised
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to mouse MIP-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Recombinant mouse MIP-2
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution.
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 6 months from date of despatch
- Guarantee
- 6 months from date of despatch
More Information
- UniProt
- P10889
- Entrez Gene
- Cxcl2
- GO Terms
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0008009 chemokine activity
- Regulatory
- For research purposes only
Applications of MIP-2 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.25 | 1.0ug/ml | |
Immunohistology - Frozen | |||
Immunohistology - Paraffin 1 | |||
Western Blotting | 0.1 | 0.2ug/ml | |
ELISA | 0.5ug/ml | ||
Functional Assays | 0.50ug/ml | 0.90ug/ml | |
Immunohistology - Frozen | |||
Immunohistology - Paraffin 1 | |||
Western Blotting | 0.1ug/ml | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended for this purpose.
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended for this purpose.
- ELISA
- Biotinylated Rabbit anti Mouse MIP-2 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with AAM48 as the capture antibody and PMP55 as the standard.
- ELISA
- Rabbit anti Mouse MIP-2 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with biotinylated Rabbit anti mouse MIP-2 antibody (AAM48B) as the detection antibody and recombinant Mouse MIP-2 protein (PMP55) as a standard.
- Western Blotting
- Biotinylated Rabbit anti Mouse MIP-2 antibody may used under either reducing or non-reducing conditions with PMP55 as the positive control.
- Western Blotting
- Rabbit anti Mouse MIP-2 antibody may be used under either reducing or non-reducing conditions with recombinant Mouse MIP-2 (PMP55) protein as a positive control.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti Mouse MIP-2 | AAM48 | C E FN P* WB | 0.1 mg |
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Rabbit anti Mouse MIP-2:Biotin | AAM48B | C E P* WB | 50 µg |
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Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Positive Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Recombinant Mouse MIP-2 | PMP55 | E FN WB | 20 µg |
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Recombinant Mouse MIP-2 | PMP55 | E FN WB | 20 µg |
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Product Specific References
References for MIP-2 antibody
-
Tittel, A.P. et al. (2011) Kidney dendritic cells induce innate immunity against bacterial pyelonephritis.
J Am Soc Nephrol. 22 (8): 1435-41. -
Roche, J.K. et al. (2007) CXCL1/KC and CXCL2/MIP-2 are critical effectors and potential targets for therapy of Escherichia coli O157:H7-associated renal inflammation.
Am J Pathol. 170: 526-37. -
Di Carlo, E. et al. (2000) The combined action of IL-15 and IL-12 gene transfer can induce tumor cell rejection without T and NK cell involvement.
J Immunol. 165: 3111-8. -
Cavallo, F. et al (2001) Interleukin 12-activated lymphocytes influence tumor genetic programs.
Cancer Res. 61: 3518-23. -
González-López, A. et al. (2011) Inflammation and matrix remodeling during repair of ventilator-induced lung injury.
Am J Physiol Lung Cell Mol Physiol. 301: L500-9. -
Lazic, M. et al. (2014) Differential regulation of inflammation and apoptosis in Fas-resistant hepatocyte-specific Bid-deficient mice.
J Hepatol. 61: 107-15. -
Nicholas, J. et al. (2015) Time course of chemokine expression and leukocyte infiltration after acute skeletal muscle injury in mice.
Innate Immun. 21 (3): 266-74. -
Yoshida, T. et al. (2014) Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.
Genes Cells. 19: 842-52. -
Lasarte, S. et al. (2016) Sex Hormones Coordinate Neutrophil Immunity in the Vagina by Controlling Chemokine Gradients.
J Infect Dis. 213 (3): 476-84.
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