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MIP-2 antibody

Product Code Applications Pack Size List Price Your Price Qty
AAM48
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SDS Safety Datasheet SDS
C E FN P * WB 0.1 mg loader
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loader

Rabbit anti Mouse MIP-2 antibody recognizes mouse macrophage inflammatory protein-2 (MIP-2), also known as CXCL2.

MIP-2 is expressed by macrophages and epidermal langerhans cells and is chemotactic for neutrophils.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to mouse MIP-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Functional Assays 0.50ug/ml 0.90ug/ml
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting 0.1ug/ml 0.2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

    Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Rabbit anti Mouse MIP-2 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with biotinylated Rabbit anti mouse MIP-2 antibody (AAM48B) as the detection antibody and recombinant Mouse MIP-2 protein (PMP55) as a standard.
Western Blotting
Rabbit anti Mouse MIP-2 antibody may be used under either reducing or non-reducing conditions with recombinant Mouse MIP-2 (PMP55) protein as a positive control.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Mouse MIP-2:Biotin AAM48B C E P * WB 50 µg loader
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Description Rabbit anti Mouse MIP-2:Biotin
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Description Product Code Applications Pack Size List Price Your Price Quantity
Recombinant Mouse MIP-2 PMP55 E FN WB 20 µg
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Description Recombinant Mouse MIP-2

References for MIP-2 antibody

  1. Di Carlo, E. et al. (2000) The combined action of IL-15 and IL-12 gene transfer can induce tumor cell rejection without T and NK cell involvement.
    J Immunol. 165: 3111-8.
  2. Cavallo, F. et al (2001) Interleukin 12-activated lymphocytes influence tumor genetic programs.
    Cancer Res. 61: 3518-23.
  3. Roche, J.K. et al. (2007) CXCL1/KC and CXCL2/MIP-2 are critical effectors and potential targets for therapy of Escherichia coli O157:H7-associated renal inflammation.
    Am J Pathol. 170: 526-37.
  4. González-López, A. et al. (2011) Inflammation and matrix remodeling during repair of ventilator-induced lung injury.
    Am J Physiol Lung Cell Mol Physiol. 301: L500-9.
  5. Tittel, A.P. et al. (2011) Kidney dendritic cells induce innate immunity against bacterial pyelonephritis.
    J Am Soc Nephrol. 22 (8): 1435-41.
  6. Yoshida, T. et al. (2014) Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.
    Genes Cells. 19: 842-52.
  7. Lazic, M. et al. (2014) Differential regulation of inflammation and apoptosis in Fas-resistant hepatocyte-specific Bid-deficient mice.
    J Hepatol. 61: 107-15.
  8. Nicholas, J. et al. (2015) Time course of chemokine expression and leukocyte infiltration after acute skeletal muscle injury in mice.
    Innate Immun. 21 (3): 266-74.
  9. View The Latest Product References
  10. Lasarte, S. et al. (2016) Sex Hormones Coordinate Neutrophil Immunity in the Vagina by Controlling Chemokine Gradients.
    J Infect Dis. 213 (3): 476-84.
  11. Salinas-Muñoz, L. et al. (2019) Estradiol impairs epithelial CXCL1 gradient in the cervix to delay neutrophil transepithelial migration during insemination.
    J Reprod Immunol. 132: 9-15.
  12. Konishi, T. et al. (2019) Cell-specific regulatory effects of CXCR2 on cholestatic liver injury.
    Am J Physiol Gastrointest Liver Physiol. 317 (6): G773-G783.
  13. Shimada, A. & Hasegawa-Ishii, S. (2021) Increased cytokine expression in the choroid plexus stroma and epithelium in response to endotoxin-induced systemic inflammation in mice.
    Toxicol Rep. 8: 520-8.

Synonyms
CXCL2
RRID
AB_2086327
UniProt
P10889
Entrez Gene
Cxcl2
GO Terms
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008009 chemokine activity

AAM48

150882 153847 164508

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