MIP-2 antibody
Rabbit anti Mouse MIP-2 antibody used for the identification of MIP-2 positive cells in mouse skin by immunohistochemistry on formailn fixed paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis of 12-O-tetradecanoylphorbol 13-acetate (TPA)-treated skin. TPA-treated skin from control (A–C) and afadin conditional knockout (CKO) (D–F) mice was stained with the antibodies shown at the top of each picture. Scale bar 50 μm.
From: Yoshida T, Iwata T, Takai Y, Birchmeier W, Yamato M, Okano T. Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice. Genes Cells. 2014 Nov;19(11):842-52.
Rabbit anti Mouse MIP-2 antibody recognizes mouse macrophage inflammatory protein-2 (MIP-2), also known as CXCL2.
MIP-2 is expressed by macrophages and epidermal langerhans cells and is chemotactic for neutrophils.
MIP-2 is expressed by macrophages and epidermal langerhans cells and is chemotactic for neutrophils.
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Biotin - lyophilised
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to mouse MIP-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant mouse MIP-2
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution.
Storage Information
- Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of reconstitution.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P10889 Related reagents
- Entrez Gene
- Cxcl2 Related reagents
- GO Terms
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0008009 chemokine activity
- Regulatory
- For research purposes only
Applications of MIP-2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.25 | 1.0ug/ml | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Western Blotting | 0.1 | 0.2ug/ml | |
| ELISA | 0.5ug/ml | ||
| Functional Assays | 0.50ug/ml | 0.90ug/ml | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Western Blotting | 0.1ug/ml | 0.2ug/ml |
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended for this purpose.
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- Biotinylated Rabbit anti Mouse MIP-2 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with AAM48 as the capture antibody and PMP55 as the standard.
- ELISA
- Rabbit anti Mouse MIP-2 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with biotinylated Rabbit anti mouse MIP-2 antibody (AAM48B) as the detection antibody and recombinant Mouse MIP-2 protein (PMP55) as a standard.
- Western Blotting
- Biotinylated Rabbit anti Mouse MIP-2 antibody may used under either reducing or non-reducing conditions with PMP55 as the positive control.
- Western Blotting
- Rabbit anti Mouse MIP-2 antibody may be used under either reducing or non-reducing conditions with recombinant Mouse MIP-2 (PMP55) protein as a positive control.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Mouse MIP-2 | AAM48 | 0.1 mg | C E FN P* WB | ||
| Rabbit anti Mouse MIP-2:Biotin | AAM48B | 50 µg | C E P* WB | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P | ||
| TidyBlot™ Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Positive Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Recombinant Mouse MIP-2 | PMP55 | 20 µg | E FN WB | ||
| Recombinant Mouse MIP-2 | PMP55 | 20 µg | E FN WB |
Product Specific References
References for MIP-2 antibody
-
Tittel, A.P. et al. (2011) Kidney dendritic cells induce innate immunity against bacterial pyelonephritis.
J Am Soc Nephrol. 22 (8): 1435-41. -
Roche, J.K. et al. (2007) CXCL1/KC and CXCL2/MIP-2 are critical effectors and potential targets for therapy of Escherichia coli O157:H7-associated renal inflammation.
Am J Pathol. 170: 526-37. -
Di Carlo, E. et al. (2000) The combined action of IL-15 and IL-12 gene transfer can induce tumor cell rejection without T and NK cell involvement.
J Immunol. 165: 3111-8. -
Cavallo, F. et al (2001) Interleukin 12-activated lymphocytes influence tumor genetic programs.
Cancer Res. 61: 3518-23. -
González-López, A. et al. (2011) Inflammation and matrix remodeling during repair of ventilator-induced lung injury.
Am J Physiol Lung Cell Mol Physiol. 301: L500-9. -
Lazic, M. et al. (2014) Differential regulation of inflammation and apoptosis in Fas-resistant hepatocyte-specific Bid-deficient mice.
J Hepatol. 61: 107-15. -
Nicholas, J. et al. (2015) Time course of chemokine expression and leukocyte infiltration after acute skeletal muscle injury in mice.
Innate Immun. 21 (3): 266-74. -
Yoshida, T. et al. (2014) Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.
Genes Cells. 19: 842-52. -
Lasarte, S. et al. (2016) Sex Hormones Coordinate Neutrophil Immunity in the Vagina by Controlling Chemokine Gradients.
J Infect Dis. 213 (3): 476-84.
