In accordance with recommendations by the International Working Group for Antibody Validation (Uhlen et al. 2016), we use various methods to validate PrecisionAb Antibodies. Our methods include Knockout (KO) antibody validation.
These include genetic strategies where the expression of the target protein is compared between the wild type cell line and the cell line in which gene encoding the protein is knocked out using CRISPR/Cas9 (Figure 1). In the KO cell line, a specific antibody doesn’t produce the signal demonstrating that the antibody detects the intended target protein.
Fig. 1. Western blot analysis of PFKP knockout HeLa (PFKP KO) and wild type HeLa (WT) whole cell lysates. Probed with A, Mouse Anti-PFKP Antibody (VMA00145) and B, hFAB Rhodamine Anti-Tubulin Primary Antibody (12004166). C, normalized signal intensity of PFKP from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.
Antibodies that have been KO validated are marked with a KO validation flag and a PrecisionAb enhanced validation logo on product webpages.
Please use the filters to sort the attributes in the table below in order to find the antibody that fits your exact requirements. If you need any further assistance please do not hesitate to contact us.
|Description||Specificity||Target||Format||Host||Isotype||Clone||Applications||Citations||Product Type||Code||Validation Types|
Learn about the validation methods implemented by Bio-Rad, including CRISPR-Cas9 gene editing, siRNA, and immunoprecipitation followed by mass spectrometry, ensuring the specificity and reliability of our antibodies in your application.
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