Knockout (KO) Validated Antibodies

Highly Validated Antibodies For Results You Can Trust

In accordance with recommendations by the International Working Group for Antibody Validation (Uhlen et al. 2016), we use various methods to validate PrecisionAb Antibodies. Our methods include Knockout (KO) antibody validation.

Knockout Validation

These include genetic strategies where the expression of the target protein is compared between the wild type cell line and the cell line in which gene encoding the protein is knocked out using CRISPR/Cas9 (Figure 1). In the KO cell line, a specific antibody doesn’t produce the signal demonstrating that the antibody detects the intended target protein.

Fig. 1. Western blot analysis of PFKP knockout HeLa (PFKP KO) and wild type HeLa (WT) whole cell lysates. Probed with A, Rabbit Anti-Annexin I Antibody (VPA00078) and B, hFAB Rhodamine Anti-Tubulin Primary Antibody (12004166). C, normalized signal intensity of Annexin I from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

KO Validated PrecisionAb Antibodies

Antibodies that have been KO validated are marked with a KO validation flag and a PrecisionAb enhanced validation logo on product webpages.

Please use the filters to sort the attributes in the table below in order to find the antibody that fits your exact requirements. If you need any further assistance please do not hesitate to contact us.

Reference

• Uhlen M et al. (2016). A proposal for validation of antibodies. Nature Methods 13, 823-827.