BDNF antibody

Rabbit anti Human BDNF

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
BDNF

Product Code Applications Pack Size List Price Your Price Qty
AHP1831
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SDS Safety Datasheet SDS
E WB 0.1 mg loader
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Rabbit anti Human BDNF antibody recognizes human brain-derived neurotrophic factor (BDNF), also known as abrineurin. BDNF is a secreted 119 amino acid ~30 kDa protein with an additional 18 amino acid signal peptide and a 110 amino acid propeptide region. BDNF is a potent neurotrophin and member of the NGF-beta family, secreted from dense core vesicles, which promotes the growth, differentiation and survival of neurons within, or directly connected to, the central nervous system (CNS).

BDNF signals through binding to the high affinity receptor trkB, and also binds to the low affinity neurotrophin receptor LNGFR. BDNF is involved in the modulation of short-term synaptic function and in the formation of long-term memory (Alonso et al. 2002). BDNF is modulated during progression of Alzheimer's with an early increase and reducel levels in patients with latter stages of Alzheimer's (Laske et al. 2005). Decreased levels of BDNF are also noted in the progression of Huntington's disease (Zuccato et al. 2008) .

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antiserum to human BDNF were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This purified human BDNF antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human BDNF antibody (AHP1831B) as the detection reagent and recombinant human BDNF (PHP213) as the standard.

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Synonyms
BRAIN-DERIVED Neurotrophic Factor
RRID
AB_2064219
UniProt
P23560
Entrez Gene
BDNF
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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