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VEGF antibody

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Rabbit anti Rat VEGF:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
VEGF

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Rabbit anti Rat VEGF antibody specifically recognizes rat VEGF (vascular endothelial growth factor), a heparin-binding cytokine and member of the PDGF/VEGF growth factor family, which acts as a vasodilator, regulator of vasculogenesis, and is a potent stimulator of angiogenesis of both normal and cancerous cells.

All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), and the functional forms of VEGF, which exist as disulphide-linked homodimers, are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF165.

Target Species
Rat
Product Form
Purified IgG conjugated to Biotin - lyophilized
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antiserum to rat VEGF was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant rat VEGF.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Biotinylated rabbit anti rat VEGF may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified rabbit anti rat VEGF antibody as the capture reagent.

Synonyms
VPF
RRID
AB_2212684
UniProt
P16612
Entrez Gene
Vegfa
GO Terms
GO:0001974 blood vessel remodeling
GO:0016020 membrane
GO:0005604 basement membrane
GO:0005615 extracellular space
GO:0008083 growth factor activity
GO:0008201 heparin binding
GO:0009409 response to cold
GO:0042088 T-helper 1 type immune response
GO:0042803 protein homodimerization activity
GO:0043117 positive regulation of vascular permeability
GO:0045779 negative regulation of bone resorption
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048255 mRNA stabilization
GO:0048286 lung alveolus development
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0051781 positive regulation of cell division
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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