VEGF antibody
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Rabbit anti Rat VEGF antibody specifically recognizes rat VEGF (vascular endothelial growth factor), a heparin-binding cytokine and member of the PDGF/VEGF growth factor family, which acts as a vasodilator, regulator of vasculogenesis, and is a potent stimulator of angiogenesis of both normal and cancerous cells.
All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), and the functional forms of VEGF, which exist as disulphide-linked homodimers, are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF165.
All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), and the functional forms of VEGF, which exist as disulphide-linked homodimers, are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF165.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Biotin - lyophilised
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to rat VEGF was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant rat VEGF.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml after reconstitution
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of reconstitution.
- Shelf Life
- 3 months from date of reconstitution.
Please see label for expiry date.
More Information
- UniProt
- P16612 Related reagents
- Entrez Gene
- Vegfa Related reagents
- GO Terms
- GO:0001974 blood vessel remodeling
- GO:0016020 membrane
- GO:0005604 basement membrane
- GO:0005615 extracellular space
- GO:0008083 growth factor activity
- GO:0008201 heparin binding
- GO:0009409 response to cold
- GO:0042088 T-helper 1 type immune response
- GO:0042803 protein homodimerization activity
- GO:0043117 positive regulation of vascular permeability
- GO:0045779 negative regulation of bone resorption
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048255 mRNA stabilization
- GO:0048286 lung alveolus development
- GO:0048661 positive regulation of smooth muscle cell proliferation
- GO:0051781 positive regulation of cell division
- Regulatory
- For research purposes only
Applications of VEGF antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5 | 2.0ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml | |
| ELISA | 0.5 | 2.0ug/ml | |
| Functional Assays | 0.05 | 0.1ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- Biotinylated rabbit anti rat VEGF may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified rabbit anti rat VEGF antibody as the capture reagent.
- ELISA
- Purified rabbit anti rat VEGF may be used in an indirect ELISA or as the capture reagent in a sandwiche ELISA with our biotinylated VEGF antibody as the detection reagent.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot™ Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
