VEGF antibody

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Rabbit anti Rat VEGF:Biotin

Rabbit anti Rat VEGF

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AAR42B	E WB			50 µg
AAR42	E FN WB			0.1 mg

Rabbit anti Rat VEGF antibody specifically recognizes rat VEGF (vascular endothelial growth factor), a heparin-binding cytokine and member of the PDGF/VEGF growth factor family, which acts as a vasodilator, regulator of vasculogenesis, and is a potent stimulator of angiogenesis of both normal and cancerous cells.

All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), and the functional forms of VEGF, which exist as disulphide-linked homodimers, are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF₁₆₅.

Product Details

Target Species: Rat
Product Form: Purified IgG conjugated to Biotin - lyophilised
Product Form: Purified IgG - lyophilised
Reconstitution: Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution: Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation: Antiserum to rat VEGF was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Preservative Stabilisers: None present
Immunogen: Recombinant rat VEGF.
Approx. Protein Concentrations: IgG concentration 0.1mg/ml after reconstitution
Approx. Protein Concentrations: IgG concentration 1.0mg/ml after reconstitution

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at -20^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Shelf Life: 3 months from date of reconstitution.

Please see label for expiry date.

More Information

UniProt: P16612 Related reagents
Entrez Gene: Vegfa Related reagents
GO Terms: GO:0001974 blood vessel remodeling; GO:0016020 membrane; GO:0005604 basement membrane; GO:0005615 extracellular space; GO:0008083 growth factor activity; GO:0008201 heparin binding; GO:0009409 response to cold; GO:0042088 T-helper 1 type immune response; GO:0042803 protein homodimerization activity; GO:0043117 positive regulation of vascular permeability; GO:0045779 negative regulation of bone resorption; GO:0045944 positive regulation of transcription from RNA polymerase II promoter; GO:0048255 mRNA stabilization; GO:0048286 lung alveolus development; GO:0048661 positive regulation of smooth muscle cell proliferation; GO:0051781 positive regulation of cell division
Regulatory: For research purposes only

Applications of VEGF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.5	2.0ug/ml
Western Blotting	0.1	0.2ug/ml
ELISA	0.5	2.0ug/ml
Functional Assays	0.05	0.1ug/ml
Western Blotting	0.1	0.2ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: Biotinylated rabbit anti rat VEGF may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified rabbit anti rat VEGF antibody as the capture reagent.
ELISA: Purified rabbit anti rat VEGF may be used in an indirect ELISA or as the capture reagent in a sandwiche ELISA with our biotinylated VEGF antibody as the detection reagent.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*

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