IL-6 antibody

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Chicken anti Bovine Interleukin-6:Biotin

Chicken anti Bovine Interleukin-6

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2380B E 50 µg
AHP2380 E WB 0.1 mg
Chicken anti Bovine interleukin-6 antibody recognizes bovine interleukin-6 (IL-6), a 23.8 kDa cytokine belonging to the IL-6 superfamily.

Bovine interleukin-6 is a multifunctional cytokine secreted by a range of cell types, in particular T lymphocytes and macrophages. IL-6 has a range of activities and includes the differentiation of B cells into Ig producing cells, differentiation of lymphocytes and of monocytes. IL-6 functions in both a pro-inflammatory role where it is an important inducer of the accute phase response and also as an anti-inflamatory myokine.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep Weak
Cat
Dog
Horse
Human
Mouse
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to bovine interleukin-6 were raised by repeated immunisation of chickens with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant bovine interleukin-6
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch

More Information

UniProt
P26892 Related reagents
Entrez Gene
IL6 Related reagents
GO Terms
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
Regulatory
For research purposes only

Applications of IL-6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.1ug/ml 0.5ug/ml
ELISA 1ug/ml 5ug/ml
Western Blotting 0.1ug/ml 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used as a detection antibody in a sandwich ELISA together with AHP2380 as the capture reagent. Recombinant Bovine interleukin-6 (PBP021) may be used as a standard.
ELISA
This product may be used as a capture antibody in a sandwich ELISA together with AHP2380B as the detection reagent. Recombinant Bovine interleukin-6 (PBP021) may be used as a standard.