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IL-1 Beta antibody

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Anti Mouse Interleukin-1 beta Antibody thumbnail image 1 Anti Mouse Interleukin-1 beta Antibody thumbnail image 2
Anti Mouse Interleukin-1 beta Antibody gallery image 1
Published customer image:
Rabbit anti Mouse interleukin-1β antibody (AAM13G) used for the detection of IL-1β in macrophages by flow cytometry
Image caption:
Sensitivity of cytokine detection using flow cytometry. Histograms and dot plots of IL-1β (A) expression in LPS-activated peritoneal macrophages versus macrophages/granulocytes isolated from cortex 24 hours after pMCAO. Light colored histograms represent cells stained with isotype control antibodies and filled histograms represent cells stained with antibodies for IL-1β (A).

From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B.
Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice.
J Neuroinflammation. 2008 Oct 23;5:46.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Mouse Interleukin-1 beta Antibody gallery image 2
Published customer image:
Rabbit anti Mouse interleukin-1β antibody (AAM13G) used for the detection of IL-1β in macrophages by immunofluorescence.
Image caption:
Co-expression of IL-1β or TNF-α with CD11b+ cells. Cortical infarction 24 hours after pMCAO leads to expression of IL-1β and TNF-α in activated CD11b+ microglia and macrophages/granulocytes. (A) Distribution of CD11b+, IL-1β+ and TNF-α+ cells in infarct (IF) and peri-infarct (P-IF) regions. IL-1β+ (B) and TNF-α+ (C) cells were particularly numerous at the edge of the infarct, and these cytokines were exclusively expressed by CD11b+ cells (arrows). CD11b+ cells were visualized using Alexa Fluor® 488-conjugated goat anti-rat IgG, IL-1β+ and TNF-α+ cells using Alexa Fluor® 594-conjugated donkey anti-rabbit IgG. Scale bars: 200 μm (A), 20 μm (B, C).

From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B.
Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice.
J Neuroinflammation. 2008 Oct 23;5:46.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Rabbit anti Mouse Interleukin-1 beta:Biotin

Rabbit anti Mouse Interleukin-1 beta

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AAM13B
Datasheet
E WB
MSDS
50 µg loader

AAM13G
Datasheet
C E FN IF WB
MSDS
0.1 mg loader

Rabbit anti Mouse Interleukin-1 beta antibody recognizes mouse Interleukin-1 beta (IL-1 beta), a cytokine involved in the inflammatory response.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to murine IL-1beta were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by antigen affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Recombinant murine IL-1 beta.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution.

Storage Information

Storage
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
6 months from date of despatch
Guarantee
6 months from date of despatch

More Information

UniProt
P10749
Entrez Gene
Il1b
GO Terms
GO:0001660 fever generation
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005615 extracellular space
GO:0030141 stored secretory granule
GO:0008083 growth factor activity
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0010829 negative regulation of glucose transport
GO:0030593 neutrophil chemotaxis
GO:0031622 positive regulation of fever generation
GO:0032308 positive regulation of prostaglandin secretion
GO:0032496 response to lipopolysaccharide
GO:0032755 positive regulation of interleukin-6 production
GO:0033198 response to ATP
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043407 negative regulation of MAP kinase activity
GO:0045080 positive regulation of chemokine biosynthetic process
GO:0045410 positive regulation of interleukin-6 biosynthetic process
GO:0045766 positive regulation of angiogenesis
GO:0046330 positive regulation of JNK cascade
GO:0046627 negative regulation of insulin receptor signaling pathway
GO:0050996 positive regulation of lipid catabolic process
GO:0051781 positive regulation of cell division
GO:0070164 negative regulation of adiponectin secretion
Regulatory
For research purposes only

Applications of IL-1 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
ELISA 0.5 2.0ug/ml
Functional Assays 0.1 0.15ug/ml
Immunofluorescence
Immunohistology - Frozen
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
ELISA
AAM13B may be used in a direct ELISA or as the detection reagent in in a sandwich ELISA with AAM13G as the capture reagent and PMP71 as the standard.
ELISA
AAM13G may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with AAM13B as the detection reagent an PMP71 as the standard.
Western Blotting
AAM13B may be used under either reducing or non-reducing conditions with PMP71 as the positive control.
Western Blotting
AAM13G may be used under either reducing or non-reducing conditions with PMP71 as the positive control.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Mouse Interleukin-1 beta AAM13G C E FN IF WB 0.1 mg loader
Rabbit anti Mouse Interleukin-1 beta:Biotin AAM13B E WB 50 µg loader
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Positive Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Recombinant Mouse Interleukin-1 beta PMP71 E FN WB 10 µg loader
Recombinant Mouse Interleukin-1 beta PMP71 E FN WB 10 µg loader

Product Specific References

References for IL-1 Beta antibody

  1. Clausen, B.H. et al. (2008) Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice.
    J Neuroinflammation. 5: 46.
  2. Ruan, L. et al. (2009) Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease.
    Curr Alzheimer Res. 6: 531-40.
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