IL-1 Beta antibody

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Rabbit anti Mouse Interleukin-1 beta:Biotin

Rabbit anti Mouse Interleukin-1 beta

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAM13B E WB 50 µg
AAM13G C E FN IF WB 0.1 mg
Rabbit anti Mouse Interleukin-1 beta antibody recognizes mouse Interleukin-1 beta (IL-1 beta), a cytokine involved in the inflammatory response.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 1.0ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to murine IL-1beta were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by antigen affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Immunogen
Recombinant murine IL-1 beta
Approx. Protein Concentrations
IgG concentration 50 ug/ml after reconstitution
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution.

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P10749 Related reagents
Entrez Gene
Il1b Related reagents
GO Terms
GO:0001660 fever generation
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005615 extracellular space
GO:0030141 stored secretory granule
GO:0008083 growth factor activity
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0010829 negative regulation of glucose transport
GO:0030593 neutrophil chemotaxis
GO:0031622 positive regulation of fever generation
GO:0032308 positive regulation of prostaglandin secretion
GO:0032496 response to lipopolysaccharide
GO:0032755 positive regulation of interleukin-6 production
GO:0033198 response to ATP
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043407 negative regulation of MAP kinase activity
GO:0045080 positive regulation of chemokine biosynthetic process
GO:0045410 positive regulation of interleukin-6 biosynthetic process
GO:0045766 positive regulation of angiogenesis
GO:0046330 positive regulation of JNK cascade
GO:0046627 negative regulation of insulin receptor signaling pathway
GO:0050996 positive regulation of lipid catabolic process
GO:0051781 positive regulation of cell division
GO:0070164 negative regulation of adiponectin secretion
Regulatory
For research purposes only

Applications of IL-1 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
ELISA 0.5 2.0ug/ml
Functional Assays 0.1 0.15ug/ml
Immunofluorescence
Immunohistology - Frozen
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
ELISA
AAM13B may be used in a direct ELISA or as the detection reagent in in a sandwich ELISA with AAM13G as the capture reagent and PMP71 as the standard.
ELISA
AAM13G may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with AAM13B as the detection reagent an PMP71 as the standard.
Western Blotting
AAM13B may be used under either reducing or non-reducing conditions with PMP71 as the positive control.
Western Blotting
AAM13G may be used under either reducing or non-reducing conditions with PMP71 as the positive control.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Mouse Interleukin-1 beta AAM13G 0.1 mg C E FN IF WB
Rabbit anti Mouse Interleukin-1 beta:Biotin AAM13B 50 µg E WB
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Positive Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Recombinant Mouse Interleukin-1 beta PMP71 10 µg E FN WB
Recombinant Mouse Interleukin-1 beta PMP71 10 µg E FN WB

Product Specific References

References for IL-1 Beta antibody

  1. Clausen, B.H. et al. (2008) Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice.
    J Neuroinflammation. 5: 46.
  2. Ruan, L. et al. (2009) Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease.
    Curr Alzheimer Res. 6: 531-40.