IL-1 Beta antibody

Rabbit anti Mouse Interleukin-1 beta

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-1 Beta

Product Code Applications Pack Size List Price Your Price Qty
AAM13G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E FN IF WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti Mouse Interleukin-1 beta antibody recognizes mouse Interleukin-1 beta (IL-1 beta), a cytokine involved in the inflammatory response.

Target Species
Mouse
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to murine IL-1beta were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by antigen affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant murine IL-1 beta.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution.
Regulatory
For research purposes only
Guarantee
6 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 0.1 0.15ug/ml
Immunofluorescence
Immunohistology - Frozen
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
ELISA
AAM13G may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with AAM13B as the detection reagent an PMP71 as the standard.
Western Blotting
AAM13G may be used under either reducing or non-reducing conditions with PMP71 as the positive control.

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Rabbit anti Mouse Interleukin-1 beta:Biotin AAM13B E WB 50 µg loader
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References for IL-1 Beta antibody

  1. Clausen, B.H. et al. (2008) Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice.
    J Neuroinflammation. 5: 46.
  2. Ruan, L. et al. (2009) Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease.
    Curr Alzheimer Res. 6: 531-40.

RRID
AB_2233635
UniProt
P10749
Entrez Gene
Il1b
GO Terms
GO:0001660 fever generation
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005615 extracellular space
GO:0030141 stored secretory granule
GO:0008083 growth factor activity
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0010829 negative regulation of glucose transport
GO:0030593 neutrophil chemotaxis
GO:0031622 positive regulation of fever generation
GO:0032308 positive regulation of prostaglandin secretion
GO:0032496 response to lipopolysaccharide
GO:0032755 positive regulation of interleukin-6 production
GO:0033198 response to ATP
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043407 negative regulation of MAP kinase activity
GO:0045080 positive regulation of chemokine biosynthetic process
GO:0045410 positive regulation of interleukin-6 biosynthetic process
GO:0045766 positive regulation of angiogenesis
GO:0046330 positive regulation of JNK cascade
GO:0046627 negative regulation of insulin receptor signaling pathway
GO:0050996 positive regulation of lipid catabolic process
GO:0051781 positive regulation of cell division
GO:0070164 negative regulation of adiponectin secretion
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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