IL-1 Beta antibody
Rabbit anti Mouse interleukin-1β antibody (AAM13G) used for the detection of IL-1β in macrophages by flow cytometry
Image caption:
Sensitivity of cytokine detection using flow cytometry. Histograms and dot plots of IL-1β (A) expression in LPS-activated peritoneal macrophages versus macrophages/granulocytes isolated from cortex 24 hours after pMCAO. Light colored histograms represent cells stained with isotype control antibodies and filled histograms represent cells stained with antibodies for IL-1β (A).
From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B.
Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice.
J Neuroinflammation. 2008 Oct 23;5:46.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Mouse interleukin-1β antibody (AAM13G) used for the detection of IL-1β in macrophages by immunofluorescence.
Image caption:
Co-expression of IL-1β or TNF-α with CD11b+ cells. Cortical infarction 24 hours after pMCAO leads to expression of IL-1β and TNF-α in activated CD11b+ microglia and macrophages/granulocytes. (A) Distribution of CD11b+, IL-1β+ and TNF-α+ cells in infarct (IF) and peri-infarct (P-IF) regions. IL-1β+ (B) and TNF-α+ (C) cells were particularly numerous at the edge of the infarct, and these cytokines were exclusively expressed by CD11b+ cells (arrows). CD11b+ cells were visualized using Alexa Fluor® 488-conjugated goat anti-rat IgG, IL-1β+ and TNF-α+ cells using Alexa Fluor® 594-conjugated donkey anti-rabbit IgG. Scale bars: 200 μm (A), 20 μm (B, C).
From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B.
Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice.
J Neuroinflammation. 2008 Oct 23;5:46.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Mouse Interleukin-1 beta antibody recognizes mouse Interleukin-1 beta (IL-1 beta), a cytokine involved in the inflammatory response.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Biotin - lyophilised
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to murine IL-1beta were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by antigen affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Recombinant murine IL-1 beta.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution.
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 6 months from date of despatch
- Guarantee
- 6 months from date of despatch
More Information
- UniProt
- P10749
- Entrez Gene
- Il1b
- GO Terms
- GO:0001660 fever generation
- GO:0005125 cytokine activity
- GO:0005149 interleukin-1 receptor binding
- GO:0005615 extracellular space
- GO:0030141 stored secretory granule
- GO:0008083 growth factor activity
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010575 positive regulation vascular endothelial growth factor production
- GO:0010829 negative regulation of glucose transport
- GO:0030593 neutrophil chemotaxis
- GO:0031622 positive regulation of fever generation
- GO:0032308 positive regulation of prostaglandin secretion
- GO:0032496 response to lipopolysaccharide
- GO:0032755 positive regulation of interleukin-6 production
- GO:0033198 response to ATP
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043407 negative regulation of MAP kinase activity
- GO:0045080 positive regulation of chemokine biosynthetic process
- GO:0045410 positive regulation of interleukin-6 biosynthetic process
- GO:0045766 positive regulation of angiogenesis
- GO:0046330 positive regulation of JNK cascade
- GO:0046627 negative regulation of insulin receptor signaling pathway
- GO:0050996 positive regulation of lipid catabolic process
- GO:0051781 positive regulation of cell division
- GO:0070164 negative regulation of adiponectin secretion
- Regulatory
- For research purposes only
Applications of IL-1 Beta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.25 | 1.0ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml | |
| ELISA | 0.5 | 2.0ug/ml | |
| Functional Assays | 0.1 | 0.15ug/ml | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Western Blotting | 0.1 | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
- ELISA
- AAM13B may be used in a direct ELISA or as the detection reagent in in a sandwich ELISA with AAM13G as the capture reagent and PMP71 as the standard.
- ELISA
- AAM13G may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with AAM13B as the detection reagent an PMP71 as the standard.
- Western Blotting
- AAM13B may be used under either reducing or non-reducing conditions with PMP71 as the positive control.
- Western Blotting
- AAM13G may be used under either reducing or non-reducing conditions with PMP71 as the positive control.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Mouse Interleukin-1 beta | AAM13G | C E FN IF WB | 0.1 mg |
|
|
| Rabbit anti Mouse Interleukin-1 beta:Biotin | AAM13B | E WB | 50 µg |
|
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| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Positive Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Recombinant Mouse Interleukin-1 beta | PMP71 | E FN WB | 10 µg |
|
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| Recombinant Mouse Interleukin-1 beta | PMP71 | E FN WB | 10 µg |
|
Product Specific References
References for IL-1 Beta antibody
-
Clausen, B.H. et al. (2008) Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice.
J Neuroinflammation. 5: 46. -
Ruan, L. et al. (2009) Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease.
Curr Alzheimer Res. 6: 531-40.
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