IL-1 Beta antibody


Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Biotin - lyophilised
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to murine IL-1beta were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by antigen affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Recombinant murine IL-1 beta.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution.
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 6 months from date of despatch
- Guarantee
- 6 months from date of despatch
More Information
- UniProt
- P10749
- Entrez Gene
- Il1b
- GO Terms
- GO:0001660 fever generation
- GO:0005125 cytokine activity
- GO:0005149 interleukin-1 receptor binding
- GO:0005615 extracellular space
- GO:0030141 stored secretory granule
- GO:0008083 growth factor activity
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010575 positive regulation vascular endothelial growth factor production
- GO:0010829 negative regulation of glucose transport
- GO:0030593 neutrophil chemotaxis
- GO:0031622 positive regulation of fever generation
- GO:0032308 positive regulation of prostaglandin secretion
- GO:0032496 response to lipopolysaccharide
- GO:0032755 positive regulation of interleukin-6 production
- GO:0033198 response to ATP
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043407 negative regulation of MAP kinase activity
- GO:0045080 positive regulation of chemokine biosynthetic process
- GO:0045410 positive regulation of interleukin-6 biosynthetic process
- GO:0045766 positive regulation of angiogenesis
- GO:0046330 positive regulation of JNK cascade
- GO:0046627 negative regulation of insulin receptor signaling pathway
- GO:0050996 positive regulation of lipid catabolic process
- GO:0051781 positive regulation of cell division
- GO:0070164 negative regulation of adiponectin secretion
- Regulatory
- For research purposes only
Applications of IL-1 Beta antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.25 | 1.0ug/ml | |
Western Blotting | 0.1 | 0.2ug/ml | |
ELISA | 0.5 | 2.0ug/ml | |
Functional Assays | 0.1 | 0.15ug/ml | |
Immunofluorescence | |||
Immunohistology - Frozen | |||
Western Blotting | 0.1 | 0.2ug/ml |
- ELISA
- AAM13B may be used in a direct ELISA or as the detection reagent in in a sandwich ELISA with AAM13G as the capture reagent and PMP71 as the standard.
- ELISA
- AAM13G may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with AAM13B as the detection reagent an PMP71 as the standard.
- Western Blotting
- AAM13B may be used under either reducing or non-reducing conditions with PMP71 as the positive control.
- Western Blotting
- AAM13G may be used under either reducing or non-reducing conditions with PMP71 as the positive control.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
![]() |
|
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
![]() |
|
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
![]() |
|
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
![]() |
|
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
![]() |
|
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
![]() |
|
Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti Mouse Interleukin-1 beta | AAM13G | C E FN IF WB | 0.1 mg |
![]() |
|
Rabbit anti Mouse Interleukin-1 beta:Biotin | AAM13B | E WB | 50 µg |
![]() |
|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Positive Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Recombinant Mouse Interleukin-1 beta | PMP71 | E FN WB | 10 µg |
![]() |
|
Recombinant Mouse Interleukin-1 beta | PMP71 | E FN WB | 10 µg |
![]() |
Product Specific References
References for IL-1 Beta antibody
-
Clausen, B.H. et al. (2008) Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice.
J Neuroinflammation. 5: 46. -
Ruan, L. et al. (2009) Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease.
Curr Alzheimer Res. 6: 531-40.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra