FGF Basic antibody


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P ResetRabbit anti Human FGF Basic
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- FGF Basic
Rabbit anti Human FGF basic polyclonal antibody recognizes human Fibroblast Growth Factor (FGF) basic, otherwise known as FGF 2 (fibroblast growth factor 2), a member of the heparin-binding growth factor family which exists in both cytosolic and nuclear isoforms, ranging in size from 18-24kDa, expressed by the majority of cells and tissues. FGF basic is a multi-functional growth factor identified as a potent inducer of angiogenesis, an important factor in wound healing, tumour vascularisation and cardiovascular disease and is pivotal for the development and maintenance of vascular integrity during embryogenesis. The recombinant basic FGF protein used as immunogen for development of Rabbit anti Human FGF basic polyclonal antibody corresponds to the C-terminal portion of the molecule (A135 - S288), present in all known isoforms of human FGF basic, thus all isoforms are expected to be recognized by this Rabbit anti Human FGF basic polyclonal antibody. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB. For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human FGF basic were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present.
- Carrier Free
- Yes
- Immunogen
- Recombinant human FGF basic (PHP105)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | ![]() |
0.5 ug/ml | 2.0 ug/ml |
Functional Assays | ![]() |
0.25 | 0.40 ug/ml |
Immunohistology - Paraffin 1 | ![]() |
0.25 | 0.5 ug/ml |
Western Blotting | ![]() |
0.1 | 0.2 ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
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References for FGF Basic antibody
-
Qian, X. et al. (1997) FGF2 concentration regulates the generation of neurons and glia from multipotent cortical stem cells.
Neuron. 18: 81-93. -
Yan, W. et al. (2017) High mechanical strength chitosan-based hydrogels cross-linked with poly(ethylene glycol)/polycaprolactone micelles for the controlled release of drugs/growth factors
J Mater Chem B. 5 (5): 961-71.
AHP1038

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