FGF Basic antibody
Rabbit anti Human bFGF antibody (AHP1038) used to assess bFGF release from hydrogels by ELISA.
Image caption:
Accumulate release (fitted curves) of bFGF from PAG/AG hydrogels within three weeks.
From: Zhao Y, Lin Z, Liu W, Piao M, Li J, Zhang H.
Controlled Release of Growth Factor from Heparin Embedded Poly(aldehyde guluronate) Hydrogels and Its Effect on Vascularization.
Gels. 2023 Jul 21;9 (7):589.
doi: 10.3390/gels9070589.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Human FGF Basic:Biotin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- FGF Basic
Antibody Quality and Performance Guarantee
| Rabbit anti Human FGF basic polyclonal antibody recognizes human Fibroblast Growth Factor (FGF) basic, otherwise known as FGF 2 (fibroblast growth factor 2), a member of the heparin-binding growth factor family which exists in both cytosolic and nuclear isoforms, ranging in size from 18-24kDa, expressed by the majority of cells and tissues. FGF basic is a multi-functional growth factor identified as a potent inducer of angiogenesis, an important factor in wound healing, tumour vascularisation and cardiovascular disease and is pivotal for the development and maintenance of vascular integrity during embryogenesis. The recombinant basic FGF protein used as immunogen for development of Rabbit anti Human FGF basic polyclonal antibody corresponds to the C-terminal portion of the molecule (A135 - S288), present in all known isoforms of human FGF basic, thus all isoforms are expected to be recognized by this Rabbit anti Human FGF basic polyclonal antibody. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - lyophilized
- Reconstitution
- Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antisera to human FGF basic were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present.
- Carrier Free
- Yes
- Immunogen
- Recombinant human FGF basic (PHP105)
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml after reconstitution.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.15 | 0.30ug/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with a Rabbit anti Human FGF basic antibody (AHP1038) as the capture reagent and E.coli derived recombinant Human FGF basic protein (PHP105) as the standard.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for FGF Basic antibody
-
Wen, Y. et al. (2017) High mechanical strength chitosan-based hydrogels cross-linked with poly(ethylene glycol)/polycaprolactone micelles for the controlled release of drugs/growth factors.
J Mater Chem B. 5 (5): 961-71. -
Zhao, Y. et al. (2023) Controlled Release of Growth Factor from Heparin Embedded Poly(aldehyde guluronate) Hydrogels and Its Effect on Vascularization.
Gels. 9(7):589.
- Synonyms
- FGF2
- RRID
- AB_2231756
- UniProt
- P09038
- Entrez Gene
- FGF2
View more products with FGF BASIC specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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