FGF Basic antibody

Rabbit anti Human FGF Basic

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
FGF Basic

Product Code Applications Pack Size List Price Your Price Qty
AHP1038
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Rabbit anti Human FGF basic polyclonal antibody recognizes human Fibroblast Growth Factor (FGF) basic, otherwise known as FGF 2 (fibroblast growth factor 2), a member of the heparin-binding growth factor family which exists in both cytosolic and nuclear isoforms, ranging in size from 18-24kDa, expressed by the majority of cells and tissues.

FGF basic is a multi-functional growth factor identified as a potent inducer of angiogenesis, an important factor in wound healing, tumour vascularisation and cardiovascular disease and is pivotal for the development and maintenance of vascular integrity during embryogenesis.

The recombinant basic FGF protein used as immunogen for development of Rabbit anti Human FGF basic polyclonal antibody corresponds to the C-terminal portion of the molecule (A135 - S288), present in all known isoforms of human FGF basic, thus all isoforms are expected to be recognized by this Rabbit anti Human FGF basic polyclonal antibody.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB. For functional studies do not add azide.
Antiserum Preparation
Antisera to human FGF basic were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present.
Carrier Free
Yes
Immunogen
Recombinant human FGF basic (PHP105)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 ug/ml 2.0 ug/ml
Functional Assays 0.25 0.40 ug/ml
Immunohistology - Paraffin 1 0.25 0.5 ug/ml
Western Blotting 0.1 0.2 ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with AHP1038B as the detection reagent and PHP105 as the standard.
Histology Positive Control Tissue
Human breast invasive ductal carcinoma

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References for FGF Basic antibody

  1. Qian, X. et al. (1997) FGF2 concentration regulates the generation of neurons and glia from multipotent cortical stem cells.
    Neuron. 18: 81-93.
  2. Yan, W. et al. (2017) High mechanical strength chitosan-based hydrogels cross-linked with poly(ethylene glycol)/polycaprolactone micelles for the controlled release of drugs/growth factors
    J Mater Chem B. 5 (5): 961-71.

Synonyms
FGF2
RRID
AB_609641
UniProt
P09038
Entrez Gene
FGF2
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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