FGF Basic antibody
Rabbit anti Human FGF basic polyclonal antibody recognizes human Fibroblast Growth Factor (FGF) basic, otherwise known as FGF 2 (fibroblast growth factor 2), a member of the heparin-binding growth factor family which exists in both cytosolic and nuclear isoforms, ranging in size from 18-24kDa, expressed by the majority of cells and tissues.
FGF basic is a multi-functional growth factor identified as a potent inducer of angiogenesis, an important factor in wound healing, tumour vascularisation and cardiovascular disease and is pivotal for the development and maintenance of vascular integrity during embryogenesis.
The recombinant basic FGF protein used as immunogen for development of Rabbit anti Human FGF basic polyclonal antibody corresponds to the C-terminal portion of the molecule (A135 - S288), present in all known isoforms of human FGF basic, thus all isoforms are expected to be recognized by this Rabbit anti Human FGF basic polyclonal antibody.
FGF basic is a multi-functional growth factor identified as a potent inducer of angiogenesis, an important factor in wound healing, tumour vascularisation and cardiovascular disease and is pivotal for the development and maintenance of vascular integrity during embryogenesis.
The recombinant basic FGF protein used as immunogen for development of Rabbit anti Human FGF basic polyclonal antibody corresponds to the C-terminal portion of the molecule (A135 - S288), present in all known isoforms of human FGF basic, thus all isoforms are expected to be recognized by this Rabbit anti Human FGF basic polyclonal antibody.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - lyophilized
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB. For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human FGF basic were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present.
- Preservative Stabilisers
- None present.
- Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Recombinant human FGF basic (PHP105)
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml after reconstitution.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 6 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
Applications of FGF Basic antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.15 | 0.30ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml | |
| ELISA | 0.5 ug/ml | 2.0 ug/ml | |
| Functional Assays | 0.25 | 0.40 ug/ml | |
| Immunohistology - Paraffin 1 | 0.25 | 0.5 ug/ml | |
| Western Blotting | 0.1 | 0.2 ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with a Rabbit anti Human FGF basic antibody (AHP1038) as the capture reagent and E.coli derived recombinant Human FGF basic protein (PHP105) as the standard.
- ELISA
- This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with AHP1038B as the detection reagent and PHP105 as the standard.
- Histology Positive Control Tissue
- Human breast invasive ductal carcinoma
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for FGF Basic antibody
-
Qian, X. et al. (1997) FGF2 concentration regulates the generation of neurons and glia from multipotent cortical stem cells.
Neuron. 18: 81-93. -
Yan, W. et al. (2017) High mechanical strength chitosan-based hydrogels cross-linked with poly(ethylene glycol)/polycaprolactone micelles for the controlled release of drugs/growth factors
J Mater Chem B. 5 (5): 961-71.
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