IL-21 antibody
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Rabbit anti Mouse Interleukin-21:Biotin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-21
Antibody Quality and Performance Guarantee
| Rabbit anti Mouse Interleukin-21 antibody recognizes mouse interleukin-21 (IL-21), a pleiotropic cytokine and member of the IL-15/IL-21 family, secreted by stimulated CD4+ T cells, which enhances stimulated B-cell proliferation/differentiation, the proliferation of bone marrow progenitor cells, and increases the activity of NK cells and disease-specific CD8+ T-cells. IL-21 signals through binding to the specific type I cytokine receptor IL-21R, coupled with the common cytokine receptor g chain (gc), initiating the activation of the JAK/STAT signalling pathway. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Biotin - lyophilized
- Reconstitution
- Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to mouse IL-21 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant mouse IL-21.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.25 | 1.0ug/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This antibody may be used in an indirect ELISA or as a detection reagent in a sandwich ELISA with AAM61 as the capture antibody.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
- RRID
- AB_2125164
- UniProt
- Q9ES17
- Entrez Gene
- Il21
- GO Terms
- GO:0001783 B cell apoptosis
- GO:0002729 positive regulation of natural killer cell cytokine production
- GO:0005125 cytokine activity
- GO:0005126 cytokine receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0032733 positive regulation of interleukin-10 production
- GO:0032825 positive regulation of natural killer cell differentiation
- GO:0045078 positive regulation of interferon-gamma biosynthetic process
- View More GO Terms
- GO:0045579 positive regulation of B cell differentiation
- GO:0045954 positive regulation of natural killer cell mediated cytotoxicity
AAM61B
100915
162556
164846
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with IL-21 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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