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Introducing New StarBright Dyes from Bio-Rad. These new, proprietary dyes have been developed specifically for flow cytometry to give you exceptional brightness with specific excitation and emission profiles. StarBright Dyes have been named by the primary excitation laser and the emission maxima to help with panel building.
StarBright Dyes are generated from different monomers, modification of the polymerization process produces exacting excitation and emission characteristics with maximal brightness. The resulting brightness allows better resolution of rare populations and low-density antigens, while the optimized excitation and emission profiles allow flexibility to fit into any multicolor flow cytometry panel.
StarBright Dyes are conjugated to our highly validated flow antibodies. Figure 1 below shows how the StarBright Violet 515 Dye compares to PE, one of the brightest dyes used in flow cytometry, and to fluorophores with similar excitation and emission profiles.
Fig. 1. StarBright Violet 515 Dye compared to other flow cytometry fluorophores. A, mouse peripheral blood was stained with CD4 PE (MCA2691PE) or CD4 StarBright Violet 515 Dye (MCA2691SBV515). B, human peripheral blood was stained with CD4 conjugated to Amethyst Orange (MCA1267AMO), Brilliant Violet 510, and StarBright Violet 515 (MCA1267SBV515) Dye. The stain index for each is shown for all three dyes. Both mouse and human samples were stained in PBS 1% BSA and analyzed on the ZE5 Cell Analyzer. Histograms show the brightness and stain index achieved with these antibodies.
StarBright Dyes easily fit into your existing protocols and panels. They are compatible with most staining protocols, work in virtually all buffers with no loss in performance, and do not require any special buffers. They can be combined with other StarBright Dyes, organic fluorophores, protein-based fluorophores, and polymer dyes without the need for special staining protocols. Furthermore, they work in special polymer buffers without a drop in performance, so can fit into existing experiments which require them.
StarBright Dyes have been developed on the ZE5 Cell Analyzer but can be used on any instrument with the right lasers and filters, including cell sorters. In addition, they work in spectral flow cytometry giving comparable results to conventional flow. The unique spectra of StarBright Dyes allows incorporation into panels that emit at similar wavelengths, which would not be possible in conventional flow cytometry.
StarBright Dyes, unlike some polymer and other dyes, are not tandem dyes and therefore exhibit no cross-laser excitation seen from the acceptor dyes in tandem dyes. They are resistant to photobleaching, are stable without loss of performance over time, and have high lot-to-lot reproducibility. Furthermore, they can be fixed with no loss of signal.
StarBright Dyes allow you to build brighter flow cytometry panels, giving you better resolution of difficult populations without the need for special buffers.
Click here for more information on all of our StarBright Dyes and how they can fit into your flow cytometry experiments.
For more information about fluorophores and immunophenotyping, refer to our flow cytometry resources.
Click on the links below to find out more in-depth information on each topic and view our popular flow cytometry basics guide.