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StarBright Dyes

Build the Best Panels with the Brightest and Biggest Range of Dyes

StarBright Dyes have been designed to be stable with superior brightness, narrow excitation and emission characteristics, and the flexibility to be included in new and existing experiments. Improved resolution of specific cell populations and minimized spillover and spreading allow you to build bigger, better panels with ease. StarBright Dyes are a great addition to Bio-Rad’s range of fluorescent dyes available for the ultraviolet, violet, and blue lasers suitable for use on all cell sorters, cell analyzers, and spectral instruments.

Benefits of StarBright Dyes

Benefits of StarBright Dyes

BrightBright

Narrow excitation and emissionNarrow excitation and emission

Flexible to work in all buffersFlexible to work in all buffers

Compatible with all instruments and reagentsCompatible with all instruments and reagents

Consistent stainingConsistent staining

On key immunophenotyping targetsOn key immunophenotyping targets

Find out more below   down arrow

StarBright Dyes Range

StarBright Dyes Range

Bio-Rad has launched multiple StarBright Dyes excitable by the blue, violet, and now ultraviolet lasers on a range of human and mouse markers and streptavidin.

StarBright Dye Resources

StarBright Dye Resources

At Bio-Rad, we provide a comprehensive range of useful antibody resources to support your research and experiments.

See all our flow resources   right arrow

New, Bright, Fluorescent Nanoparticles Developed for Flow Cytometry

StarBright Dyes are new, proprietary fluorescent nanoparticles conjugated to Bio-Rad's highly validated antibodies. This video demonstrates StarBright Dyes’ exceptional brightness, narrow excitation and emission, and stability with high lot-to-lot reproducibility. You will also discover how easily they can fit into your flow cytometry experiments and how they can be used in immunophenotyping panels to obtain improved results compared to conventional dyes.

Benefits of StarBright Dyes

Exceptional Brightness

Designed to be as bright, or brighter than competitor dyes. The exceptional brightness of StarBright Dyes allows detection of rare and low antigen density populations with excellent separation of positive from negative populations.

Fig. 1. StarBright Dyes are brighter than dyes with similar maximal emission. Click to enlarge

Fig. 1. StarBright Dyes are designed to be brighter than competitor dyes. A, examples of CD4 staining for 488, 405, and 355 nm excitable StarBright Dyes compared to competitors. B, stain index of human peripheral blood stained with various 405 nm excitable StarBright, Brilliant Violet and Super Bright Dyes conjugated to CD4. Data generated on the ZE5 Cell Analyzer.

Fig. 1. StarBright Dyes are brighter than dyes with similar maximal emission.

Narrow Excitation and Emission

Narrow emission spectra to minimize spillover and reduced excitation by other common laser lines make StarBright Dyes an ideal choice for inclusion in multicolor panels. Use our Spectraviewer to find the right StarBright Dye for your experiment.

Fig. 2. Examples of improved spectral characteristics of StarBright Dyes. Enlarge Image

Fig. 2. Examples of improved spectral characteristics of StarBright Dyes. Reduced excitation of StarBright Blue 700 Dye, compared with PerCP-Cy5.5 (red circle), and reduced excitation by the 355 nm laser with narrow emission for StarBright Violet 515 Dye, compared to Brilliant Violet 510 (blue circles).

Fig. 2. Examples of improved spectral characteristics of StarBright Dyes.

Flexible to Work in All Buffers

StarBright Dyes have no drop in performance regardless of the staining buffer, and do not require a special staining buffer when multiplexing with each other. However, if special buffers are required due to the presence of multiple polymer dyes in your panel, the flexibility of StarBright Dyes means you will still achieve bright, reliable staining.

Fig. 3. Examples of staining of human peripheral blood in different buffers. Enlarge Image

Fig. 3. Examples of staining of human peripheral blood in different buffers. A, no change in CD4 staining (MCA1267SBV670) is observed, regardless of the buffer. B, StarBright Dyes, in this case CD4SBV790 (MCA1267SBV790), work well in both Bio-Rad Staining Buffer or Brilliant Staining Buffer with one Brilliant Violet Dye. Data generated on the ZE5 Cell Analyzer.

Fig. 3. Examples of staining of human peripheral blood in different buffers.

Compatible with All Instruments and Reagents

Designed for multiplexing using the ZE5 Cell Analyzer, StarBright Dyes are suitable for cell analysis or cell sorting on any instrument with the appropriate lasers and filters. StarBright Dyes are compatible with all fluorescently-labeled antibodies and can even be added to premixed cocktails without the need for experimental changes. Furthermore, their unique spectra make them ideal for incorporating into spectral flow cytometry panels with novel dye combinations now possible.

Below are thumbnails of posters which show examples of high parameter panels in conventional and full spectrum flow cytometry. These, along with other useful flow cytometry posters, can be downloaded for your own use.

The Use of New StarBright Dyes in Spectral Flow Cytometry

The Use of New StarBright Dyes in Spectral Flow Cytometry

27-color spectral panel built using StarBright Dyes and a spectral flow cytometer.

Introducing StarBright Dyes, Novel Ultra Violet, Violet, and Blue Laser Excitable Fluorescent Nanoparticles Suitable for Immunophenotyping in Flow Cytometry

Introducing StarBright Dyes, Novel Ultra Violet, Violet, and Blue Laser Excitable Fluorescent Nanoparticles Suitable for Immunophenotyping in Flow Cytometry

18-color panel built using StarBright Dyes and the ZE5 Cell Analyzer.

View and Download These Useful Posters

Consistent Staining

Designed with minimum lot-to-lot variability, StarBright Dyes give the same level of staining and population identification regardless of the batch. Unlike some polymer and other dyes, they are not traditional tandem dyes and therefore exhibit less cross-laser excitation, emitted by the donor and acceptors in tandem dyes, due to insufficient FRET. They are resistant to photobleaching, stable without loss of performance over time, and can be fixed in paraformaldehyde or alcohol-based fixatives with no drop in signal or change in their spectral characteristics. Conveniently, they can even be premixed for up to 28 days without loss of performance for use at a later date. To help you get consistently reproducible results, refer to the StarBright Dyes staining protocol.

Fig. 4. StarBright Dyes — consistent, stable, and fixable. Enlarge Image

Fig. 4. StarBright Dyes — consistent, stable, and fixable. StarBright Dyes show consistent results regardless of the lot. A, multiple batches were used to stain peripheral blood mononuclear cells with CD4SBV515 (MCA126SBV515) and the results overlaid on a histogram. Dotted line is CD4BV510 for comparison. B, CD14SBV610 (MCA1267) was left at room temperature in the dark or in the light for up to 14 days prior to staining human peripheral blood, and the staining compared to antibody stored correctly in the fridge. A reduction in staining was only seen after 14 days storage at room temperature in the light. C, fixation in Bio-Rad Fixation Buffer or 2% PFA in PBS has no effect on the stain index compared to freshly stained samples. Data generated on the ZE5 Cell Analyzer.

Fig. 4. StarBright Dyes — consistent, stable, and fixable.

On Common Immunophenotyping Targets

StarBright Dyes are available directly conjugated to antibodies validated for flow cytometry to detect human and mouse targets, and streptavidin for binding to biotinylated antibodies. These antibodies are well-known clones which are highly cited, giving you confidence in your experiments.

Fig. 5. Examples of staining of common immunophenotyping markers using StarBright Dyes Enlarge Image

Fig. 5. Examples of staining of common immunophenotyping markers using StarBright Dyes. Human peripheral blood was stained for CD3 (MCA463SBUV400), CD19 (MCA1940SBUV605), CD4 (MCA1267SBUV510), CD8 (MCA1226SBV710), CD25 (MCA2127SBV440), and CD127 (HCA145A647) to identify B and T lymphocytes and T helper, T cytotoxic cells, and T regulatory cells within the T cell population. Classical, intermediate, and nonclassical monocytes were identified using CD14 (MCA16568SBV790) and CD16 (MCA2537A700). Further characterization of the T helper cells was obtained from CD45RA (MCA88SBV515) and CD45RO (MCA461SBV610) to identify naïve and memory cells, and the co-stimulation marker expression on T regulatory cells was measured by CD27 (MCA755SBV670) and CD28 (MCA709SBUV575) levels. Data generated on the ZE5 Cell Analyzer.

Fig. 5. Examples of staining of common immunophenotyping markers using StarBright Dyes

Useful StarBright Dye Information

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StarBright Dyes Posters

StarBright Dyes Posters

These four posters showcase the properties and benefits of StarBright Dyes in large multicolor panel experiments and their incredible performance in conventional and spectral flow cytometry.

CD4 antibody | RPA-T4

CD4 antibody | RPA-T4

Mouse Anti-Human CD4 clone RPA-T4 detects the surface glycoprotein human CD4 primarily expressed on T helper lymphocytes, T regulatory lymphocytes, peripheral blood monocytes, and tissue macrophages. Conjugated to all StarBright Dyes, CD4 is ideal for calculating and comparing stain indices between fluorescent dyes.

Introducing StarBright Dyes Webinar

Introducing StarBright Dyes Webinar

In this webinar, we will demonstrate StarBright Dyes’ exceptional brightness, narrow excitation and emission, and stability with high lot-to-lot reproducibility. You will also discover how easily they can fit into your flow cytometry experiments.

StarBright Dye Brochure

StarBright Dye Brochure

Find out what dyes are available, including example data in both conventional and spectral flow cytometry.

Flow Cytometry Basics Guide

Flow Cytometry Basics Guide

The new, updated Flow Cytometry Basics Guide has it all covered. From an introduction to basic flow cytometry principles, practical advice for planning and optimizing your experiments, to examples of conventional and spectral multicolor panels. 

CD8SBB700 antibody | LT8

CD8SBB700 antibody | LT8

Mouse Anti-Human CD8 recognizes the human CD8 cell surface glycoprotein expressed by a subset of peripheral blood T cells which express cytotoxic/suppressor activity and weakly expressed on NK cells.

CD14SBV515 antibody | TÜK4

CD14SBV515 antibody | TÜK4

Mouse Anti-Human CD14 recognizes the human cell surface antigen CD14 which functions as a pattern recognition receptor in innate immunity for a variety of ligands. It is strongly expressed on the surface of monocytes and macrophages but has also been shown to be expressed on the surface of non-myeloid cells.

Flow Cytometry Workbook

Flow Cytometry Workbook

This comprehensive course provides an introduction to flow cytometry and covers essential considerations when planning your first experiments, helping ensure successful flow cytometry and trustworthy data. To test your understanding, take a quiz at the end of each module.

Customized Flow Cytometry Panel Building Service

Customized Flow Cytometry Panel Building Service

Building multicolor flow cytometry panels can be a daunting process as it can be complex and time consuming.

Simply complete the form and we will be in touch to help build your panel free of charge.