Antibody protocols

Antibody Protocols

Flow Cytometry Protocols

Flow Cytometry Protocols

Protocols for detecting cell surface and intracellular markers

  1. Direct immunofluorescence staining of cells and blood for Flow Cytometry
  2. Indirect immunofluorescence staining of cells and blood for Flow Cytometry
  3. Direct Immunofluorescence Staining of Immunoglobulin Light Chains on B Lymphocytes in Whole Blood by Flow Cytometry
  4. Direct staining of intracellular antigens by Flow Cytometry (using LEUCOPERM™)
  5. Direct staining of intracellular antigens by Flow Cytometry: methanol method 
  6. Direct Staining of Intracellular Cytokines by Flow Cytometry 
  7. Preparation of Cells for Flow Cytometry 
  8. Whole Blood Protocol for Analysis of Intracellular Cytokines by Flow Cytometry 
  9. Digitonin Permeabilisation of Cells for Flow Cytometry 
  10. Direct Staining of Intracellular Antigens by Flow Cytometry - the paraformaldehyde/saponin method
  11. BrdU staining of cells for cell cycle analysis
  12. Propidium iodide staining of cells for cell cycle analysis
  13. Immunofluorescence staining of cells in combination with PI staining of cells for cell cycle analysis
  14. BrdU staining of cells for cell cycle analysis and apoptosis
  15. Unprimed T Cell Activation - Pharmacologic Methods
  16. Unprimed T Cell Activation - Antibody Stimulation Methods
  17. Primed T Cell Activation - Antigen Presenting Cell Co-Culture
  18. Measuring Cell Proliferation Using Cell Permeable Dyes
  19. BrdU staining of cells for cell cycle analysis with Mouse Anti-BrdU Antibody (Bu20a)
  20. Direct Staining of Intracellular CD68: Leucoperm™ Accessory Reagent Method
  21. BrdU staining of cells for cell cycle analysis with Rabbit Anti-BrdU Antibody

Functional Assay (FN) Protocol

  1. Thymocyte Il-2/PHA Co-Stimulation Assay
Western Blot Protocols

Western Blot (WB) Protocol

  1. Western Blotting protocol