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MCM2
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alamarBlue Protocols
Detailed guides to help you use alamarBlue
QC test for alamarBlue
Method to determine LD50 using alamarBlue
General method for measuring cytotoxicity or proliferation using alamarBlue (Spectrophotometry method and Fluorescence method)
Method for determining optimum length of incubation and plating density
Spectrophotometry calculations for using different filters
alamarBlue example experiments
MCM2 Protocol
Detailed guide to help you use MCM2
Rabbit anti-MCM2 Proliferation Pairs Staining Procedure
ELISA Protocols
General ELISA Protocols
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Sandwich ELISA with direct detection
Protocols for use with Anti-biotherapeutic Antibodies
PK bridging ELISA measuring free drug
PK bridging ELISA measuring total drug
PK antigen capture ELISA measuring bound drug exclusively
ADA bridging ELISA
Flow Cytometry Protocols
Protocols for detecting cell surface and intracellular markers
Direct immunofluorescence staining of cells and blood for Flow Cytometry
Indirect immunofluorescence staining of cells and blood for Flow Cytometry
Direct immunofluorescence staining of immunoglobulin light chains on B Lymphocytes in whole blood by Flow Cytometry
Direct staining of intracellular antigens by Flow Cytometry (using LEUCOPERM)
Direct staining of intracellular antigens by Flow Cytometry: methanol method
Direct staining of intracellular cytokines by Flow Cytometry
Preparation of cells for Flow Cytometry
Whole blood protocol for analysis of intracellular cytokines by Flow Cytometry
Digitonin permeabilisation of cells for Flow Cytometry
Direct staining of intracellular antigens by Flow Cytometry - the paraformaldehyde/saponin method
BrdU staining of cells for cell cycle analysis
Propidium iodide staining of cells for cell cycle analysis
Immunofluorescence staining of cells in combination with PI staining of cells for cell cycle analysis
BrdU staining of cells for cell cycle analysis and apoptosis
Unprimed T Cell activation - pharmacologic methods
Unprimed T Cell activation - antibody stimulation methods
Primed T Cell activation - Antigen presenting cell co-culture
Measuring cell proliferation using cell permeable dyes
BrdU staining of cells for cell cycle analysis with Mouse Anti-BrdU Antibody (Bu20a)
Direct staining of intracellular CD68: Leucoperm Accessory Reagent Method
BrdU staining of cells for cell cycle analysis with Rabbit Anti-BrdU Antibody
Direct immunofluorescence staining of cells with StarBright Dyes
Immunofluorescence (IF) Protocol
Fluorescence microscopy (Direct immunofluorescence) for cell suspensions
BrdU Labeling of heLa cells followed by immunostaining with Mouse Anti-BrdU Antibody
BrdU labeling of heLa cells followed by immunostaining with Rabbit Anti-BrdU Antibody
Immunohistochemistry (IHC) Protocols
Protocols for IHC-frozen and IHC-paraffin
Indirect Immunostaining of frozen tissue sections
Streptavidin-biotin immunostaining of frozen tissue sections
PAP/APAAP Immunostaining of frozen tissue sections
Indirect immunostaining of paraffin-embedded tissue sections
Streptavidin-biotin immunostaining of paraffin-embedded tissue sections
PAP/APAAP immunostaining of paraffin embedded tissue sections
Antigen retrieval techniques for use with formalin-fixed paraffin-embedded sections
Antigen retrieval techniques: For use with F4/80 immunostaining of paraffin-embedded sections
Fixative Recipes
Western Blot (WB) Protocol
Western Blotting protocol
Detection of phosphorylated proteins by Western Blotting
Method to avoid interference from denatured IgG using TidyBlot Western Blot detection reagent