Recommended Antibody Protocols

Flow Cytometry Protocols

Protocols for detecting cell surface and intracellular markers

  1. Preparation of tissue culture cell lines for flow cytometry
  2. Preparation of peripheral blood mononuclear cells for flow cytometry
  3. Preparation of peritoneal macrophages, bone marrow, thymus, and spleen cells for flow cytometry
  4. Direct immunofluorescence staining of surface epitopes of cells and whole blood
  5. Indirect immunofluorescence staining of surface epitopes of cells and whole blood
  6. Direct immunofluorescence staining of immunoglobulin light chains on B Lymphocytes in whole blood by flow cytometry
  7. Direct staining of intracellular antigens by flow cytometry (using LEUCOPERM)
  8. Direct staining of intracellular antigens by flow cytometry: methanol method 
  9. Direct staining of intracellular cytokines by flow cytometry 
  10. Whole blood protocol for analysis of intracellular cytokines by flow cytometry 
  11. Digitonin permeabilisation of cells for flow cytometry 
  12. Direct staining of intracellular antigens by flow cytometry - the paraformaldehyde/saponin method
  13. BrdU staining of cells for cell cycle analysis
  14. Propidium iodide staining of cells for cell cycle analysis
  15. Immunofluorescence staining of cells in combination with PI staining of cells for cell cycle analysis
  16. BrdU staining of cells for cell cycle analysis and apoptosis
  17. Unprimed T Cell activation - pharmacologic methods
  18. Unprimed T Cell activation - antibody stimulation methods
  19. Primed T Cell activation - Antigen presenting cell co-culture
  20. Measuring cell proliferation using cell permeable dyes
  21. BrdU staining of cells for cell cycle analysis with Mouse Anti-BrdU Antibody (Bu20a)
  22. Direct staining of intracellular CD68: Leucoperm Accessory Reagent Method
  23. BrdU staining of cells for cell cycle analysis with Rabbit Anti-BrdU Antibody
  24. Direct immunofluorescence staining of cells with StarBright Dyes
  25. Direct immunofluorescence staining of intracellular antigens using antibodies labeled with StarBright Dyes