alamarBlue Protocols

Detailed guides to help you use alamarBlue

  1. QC test for alamarBlue
  2. Method to determine LD50 using alamarBlue
  3. General method for measuring cytotoxicity or proliferation using alamarBlue (spectrophotometry method and fluorescence method)
  4. Method for determining optimum length of incubation and plating density
  5. Spectrophotometry calculations for using different filters
  6. alamarBlue example experiments

MCM2 Protocol

Detailed guide to help you use MCM2

  1. Rabbit anti-MCM2 proliferation pairs staining procedure

ELISA Protocols

General ELISA protocols

  1. Indirect
  2. Competition (Inhibition)
  3. Direct ELISA with streptavidin-biotin detection
  4. Sandwich ELISA with streptavidin-biotin detection
  5. Sandwich ELISA with direct detection

Protocols for use with anti-biotherapeutic antibodies

  1. PK bridging ELISA measuring free drug
  2. PK bridging ELISA measuring total drug
  3. PK antigen capture ELISA measuring bound drug exclusively
  4. ADA bridging ELISA

Flow Cytometry Protocols

  1. Preparation of tissue culture cell lines for flow cytometry
  2. Preparation of peripheral blood mononuclear cells for flow cytometry
  3. Preparation of peritoneal macrophages, bone marrow, thymus, and spleen cells for flow cytometry
  4. Direct immunofluorescence staining of surface epitopes of cells and whole blood
  5. Indirect immunofluorescence staining of surface epitopes of cells and whole blood
  6. Direct immunofluorescence staining of immunoglobulin light chains on B lymphocytes in whole blood
  7. Direct immunofluorescence staining of intracellular antigens: the Leucoperm method
  8. Direct immunofluorescence staining of intracellular antigens: the methanol plus Leucoperm method
  9. Direct immunofluorescence staining of intracellular cytokines in whole blood
  10. Direct immunofluorescence staining of intracellular antigens: the digitonin method
  11. Direct immunofluorescence staining of intracellular antigens: the paraformaldehyde/saponin method
  12. Propidium iodide staining of cells for cell cycle analysis
  13. Propidium iodide staining of cells for cell cycle analysis in combination with direct immunofluorescence intracellular staining
  14. BrdU staining of cells for cell cycle analysis
  15. Unprimed T-cell activation: pharmacologic methods
  16. Unprimed T-cell activation: antibody stimulation method
  17. Primed T-cell activation: antigen-presenting cell co-culture method
  18. Measuring cell proliferation using CytoTack, a cell-permeable dye
  19. Direct immunofluorescence staining of intracellular antigens using antibodies labeled with StarBright™ Dyes
  20. Direct immunofluorescence staining of whole blood for intracellular CD68: the Leucoperm method
  21. Direct immunofluorescence staining of cells with StarBright Dye-conjugated antibodies

Immunofluorescence (IF) Protocols

  1. Fluorescence microscopy (Direct immunofluorescence) for cell suspensions
  2. BrdU Labeling of heLa cells followed by immunostaining with Mouse Anti-BrdU Antibody
  3. BrdU labeling of heLa cells followed by immunostaining with Rabbit Anti-BrdU Antibody

Immunohistochemistry (IHC) Protocols

Protocols for IHC-frozen and IHC-paraffin

  1. Indirect Immunostaining of frozen tissue sections 
  2. Streptavidin-biotin immunostaining of frozen tissue sections 
  3. PAP/APAAP Immunostaining of frozen tissue sections 
  4. Indirect immunostaining of paraffin-embedded tissue sections 
  5. Streptavidin-biotin immunostaining of paraffin-embedded tissue sections 
  6. PAP/APAAP immunostaining of paraffin embedded tissue sections 
  7. Antigen retrieval techniques for use with formalin-fixed paraffin-embedded sections 
  8. Antigen retrieval techniques: For use with F4/80 immunostaining of paraffin-embedded sections 
  9. Fixative Recipes

Western Blot (WB) Protocols

  1. Western Blotting protocol 
  2. Detection of phosphorylated proteins by Western Blotting
  3. Method to avoid interference from denatured IgG using TidyBlot Western Blot detection reagent
  4. Fluorescent Western Blotting Protocol Using StarBright Dye Secondary Antibodies