IL-6 antibody
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P IF ResetRabbit anti Human Interleukin-6
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-6
Antibody Quality and Performance Guarantee
| Rabbit anti Human Interleukin-6 antibody is specific for human IL-6 (Interleukin-6), a 184 amino acid multifunctional pro-inflammatory cytokine, secreted by activated T cells, macrophages, endothelial cells, fibroblasts and osteoblasts. The diverse biological properties of IL-6 include the principle inducer of the acute phase response in the liver, stimulation of B cell differentiation and subsequent immunoglobulin production, promotion of hybridoma growth, regulation of bone metabolism and stimulation of energy mobilization in muscle and fatty tissue. IL-6 signals through a membrane bound receptor complex consisting of the ligand-binding IL-6Rα chain (CD126) and the signal-transducing component gp130 (CD130), but can also stimulate cells expressing gp130 through a soluble IL-6R. Rabbit anti Human Interleukin-6 antibody has been shown to inhibit the biological activity of Human IL-6. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to human IL-6 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human IL-6 (PHP045)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5ug/ml | |
| Functional Assays 1 | |
0.20 | 0.40ug/ml |
| Immunofluorescence | |
||
| Immunohistology - Paraffin 2 | |
0.25 | 0.5ug/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
- 1 To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-6 (1.0 ng/ml).
- 2This product requires heat-mediated antigen retrieval prior to staining paraffin-embedded sections. Citrate buffer, pH6.0, is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- Purified rabbit anti human IL-6 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with biotinylated rabbit anti human IL-6 (AHP1040B) as the detection reagent and recombinant human IL-6 (PHP045) as the standard.
- Western Blotting
- Rabbit anti human IL-6 may used under either reducing or non-reducing conditions with recombinant human IL-6 (PHP045) as the positive control
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|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Recombinant Human Interleukin-6 | PHP045 | E FN WB | 20 µg | Log in | |||
| List Price | Your Price | ||||||
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| Description | Recombinant Human Interleukin-6 | ||||||
Further Reading
-
Yasukawa, K. et al. (1987) Structure and expression of human B cell stimulatory factor-2 (BSF-2/IL-6) gene.
EMBO J. 6 (10): 2939-45. -
Peters, M. et al. (1998) Interleukin-6 and soluble interleukin-6 receptor: direct stimulation of gp130 and hematopoiesis.
Blood. 92 (10): 3495-504.
- RRID
- AB_617521
- UniProt
- P05231
- Entrez Gene
- IL6
- GO Terms
- GO:0001781 neutrophil apoptosis
- GO:0002384 hepatic immune response
- GO:0002446 neutrophil mediated immunity
- GO:0002548 monocyte chemotaxis
- GO:0002675 positive regulation of acute inflammatory response
- GO:0002690 positive regulation of leukocyte chemotaxis
- GO:0006953 acute-phase response
- GO:0005125 cytokine activity
- GO:0005138 interleukin-6 receptor binding
- View More GO Terms
- GO:0005615 extracellular space
- GO:0005896 interleukin-6 receptor complex
- GO:0006959 humoral immune response
- GO:0008083 growth factor activity
- GO:0050830 defense response to Gram-positive bacterium
- GO:0050829 defense response to Gram-negative bacterium
- GO:0008285 negative regulation of cell proliferation
- GO:0010574 regulation of vascular endothelial growth factor production
- GO:0010888 negative regulation of lipid storage
- GO:0045727 positive regulation of translation
- GO:0030168 platelet activation
- GO:0031018 endocrine pancreas development
- GO:0031175 neuron projection development
- GO:0032494 response to peptidoglycan
- GO:0032722 positive regulation of chemokine production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0032966 negative regulation of collagen biosynthetic process
- GO:0033138 positive regulation of peptidyl-serine phosphorylation
- GO:0042102 positive regulation of T cell proliferation
- GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
- GO:0043066 negative regulation of apoptosis
- GO:0043193 positive regulation of gene-specific transcription
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0045079 negative regulation of chemokine biosynthetic process
- GO:0045599 negative regulation of fat cell differentiation
- GO:0045669 positive regulation of osteoblast differentiation
- GO:0045765 regulation of angiogenesis
- GO:0045768 positive regulation of anti-apoptosis
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048661 positive regulation of smooth muscle cell proliferation
- GO:0050871 positive regulation of B cell activation
- GO:0051024 positive regulation of immunoglobulin secretion
- GO:0051091 positive regulation of transcription factor activity
- GO:0051384 response to glucocorticoid stimulus
- GO:0051607 defense response to virus
- GO:0070091 glucagon secretion
- GO:0070102 interleukin-6-mediated signaling pathway
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