IL-6 antibody

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Rabbit anti Human Interleukin-6:Biotin

Rabbit anti Human Interleukin-6

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1040B E WB 50 µg
AHP1040 E FN* IF P* WB 0.1 mg
Rabbit anti Human Interleukin-6 antibody is specific for human IL-6 (Interleukin-6), a 184 amino acid multifunctional pro-inflammatory cytokine, secreted by activated T cells, macrophages, endothelial cells, fibroblasts and osteoblasts.

The diverse biological properties of IL-6 include the principle inducer of the acute phase response in the liver, stimulation of B cell differentiation and subsequent immunoglobulin production, promotion of hybridoma growth, regulation of bone metabolism and stimulation of energy mobilisation in muscle and fatty tissue. IL-6 signals through a membrane bound receptor complex consisting of the ligand-binding IL-6Rα chain (CD126) and the signal-transducing component gp130 (CD130), but can also stimulate cells expressing gp130 through a soluble IL-6R.

Rabbit anti Human Interleukin-6 antibody has been shown to inhibit the biological activity of Human IL-6.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to human IL-6 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Immunogen
Recombinant human IL-6 (PHP045)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored udiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P05231 Related reagents
Entrez Gene
IL6 Related reagents
GO Terms
GO:0001781 neutrophil apoptosis
GO:0002384 hepatic immune response
GO:0002446 neutrophil mediated immunity
GO:0002548 monocyte chemotaxis
GO:0002675 positive regulation of acute inflammatory response
GO:0002690 positive regulation of leukocyte chemotaxis
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0005896 interleukin-6 receptor complex
GO:0006959 humoral immune response
GO:0008083 growth factor activity
GO:0050830 defense response to Gram-positive bacterium
GO:0050829 defense response to Gram-negative bacterium
GO:0008285 negative regulation of cell proliferation
GO:0010574 regulation of vascular endothelial growth factor production
GO:0010888 negative regulation of lipid storage
GO:0045727 positive regulation of translation
GO:0030168 platelet activation
GO:0031018 endocrine pancreas development
GO:0031175 neuron projection development
GO:0032494 response to peptidoglycan
GO:0032722 positive regulation of chemokine production
GO:0032755 positive regulation of interleukin-6 production
GO:0032966 negative regulation of collagen biosynthetic process
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0042102 positive regulation of T cell proliferation
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0043066 negative regulation of apoptosis
GO:0043193 positive regulation of gene-specific transcription
GO:0043410 positive regulation of MAPKKK cascade
GO:0045079 negative regulation of chemokine biosynthetic process
GO:0045599 negative regulation of fat cell differentiation
GO:0045669 positive regulation of osteoblast differentiation
GO:0045765 regulation of angiogenesis
GO:0045768 positive regulation of anti-apoptosis
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0050871 positive regulation of B cell activation
GO:0051024 positive regulation of immunoglobulin secretion
GO:0051091 positive regulation of transcription factor activity
GO:0051384 response to glucocorticoid stimulus
GO:0051607 defense response to virus
GO:0070091 glucagon secretion
GO:0070102 interleukin-6-mediated signaling pathway
Regulatory
For research purposes only

Applications of IL-6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.15 0.30ug/ml
Western Blotting 0.1 0.2ug/ml
ELISA 0.5ug/ml
Functional Assays 1 0.20 0.40ug/ml
Immunofluorescence
Immunohistology - Paraffin 2 0.25 0.5ug/ml
Western Blotting 0.1 0.2ug/ml
  1. 1 To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-6 (1.0 ng/ml).
  2. 2This product requires heat-mediated antigen retrieval prior to staining paraffin-embedded sections. Citrate buffer, pH6.0, is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This biotinylated rabbit anti human IL-6 may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human IL-6 antibody (AHP1040) as the capture reagent and recombinant human IL-6 (PHP045) as the standard.
ELISA
Purified rabbit anti human IL-6 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with biotinylated rabbit anti human IL-6 (AHP1040B) as the detection reagent and recombinant human IL-6 (PHP045) as the standard.
Western Blotting
Rabbit anti human IL-6 may used under either reducing or non-reducing conditions with recombinant human IL-6 (PHP045) as the positive control
Western Blotting
Rabbit anti human IL-6 may used under either reducing or non-reducing conditions with recombinant human IL-6 (PHP045) as the positive control

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Human Interleukin-6 AHP1040 0.1 mg E FN* IF P* WB
Rabbit anti Human Interleukin-6:Biotin AHP1040B 50 µg E WB
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Positive Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Recombinant Human Interleukin-6 PHP045 20 µg E FN WB
Recombinant Human Interleukin-6 PHP045A 5 µg E FN WB
Recombinant Human Interleukin-6 PHP045 20 µg E FN WB
Recombinant Human Interleukin-6 PHP045A 5 µg E FN WB

Product Specific References

Further Reading

  1. Yasukawa, K. et al. (1987) Structure and expression of human B cell stimulatory factor-2 (BSF-2/IL-6) gene.
    EMBO J. 6 (10): 2939-45.
  2. Peters, M. et al. (1998) Interleukin-6 and soluble interleukin-6 receptor: direct stimulation of gp130 and hematopoiesis.
    Blood. 92 (10): 3495-504.