StarBright UltraViolet Dyes

Excitable by the 355 nm laser, StarBright UltraViolet (SBUV) Dyes are a perfect way to expand your flow cytometry panels, giving you more flexibility in your panel design while avoiding spillover and spreading. Available with maximal emissions ranging from 400 to 800 nm, eight additional dyes are coming soon with the same exceptional properties as the other StarBright Dyes and are conjugated to our validated antibodies. Suitable for use in conventional and full spectrum flow cytometry, these bright and stable dyes can be used in any buffer and have narrow excitation and emission profiles enabling you to build bigger and better panels.

SBUV Dye Range

Table 1. StarBright UltraViolet range.

StarBright Dye

Max Ex, nm

Max Em, nm

Brightness (15)

ZE5 Cell Analyzer Optimal Filter

Comparison Dye

Launch Date

Flier

StarBright UltraViolet 400 Dye

335

394

3

387/11

Brilliant Ultraviolet (BUV) 395

Available now

SBUV400 PDF

StarBright UltraViolet 445 Dye

347

440

3

447/60 or 460/22

Alexa Fluor 350, Dylight 350

Available now

SBUV445 PDF

StarBright UltraViolet 510 Dye

340

513

3

509/25 or 525/50

BUV496

Available now

SBUV510 PDF

StarBright UltraViolet 575 Dye

340

569

4

577/15

BUV563

Coming soon

Coming soon

StarBright UltraViolet 605 Dye

340

609

4

615/24

BUV615

Coming soon

Coming soon

StarBright UltraViolet 665 Dye

340

669

4

670/30

BUV661

Available now

SBUV665 PDF

StarBright UltraViolet 740 Dye

344

743

4

747/33

BUV737

Coming soon

Coming soon

StarBright UltraViolet 795 Dye

340

792

2

780LP

BUV805

Available now

SBUV795 PDF


Fig. 1. Excitation and emission spectra of StarBright UltraViolet Dyes.


Fig. 1. Excitation and emission spectra of StarBright UltraViolet Dyes.


StarBright UltraViolet 400 (SBUV400) Dye

SBUV400 Dye is a bright, 355 nm laser excitable dye that has all the benefits of StarBright Dyes and is suitable for use in conventional and full spectrum flow cytometry (Figure 2).

Fig 2. Brightness comparison.


Fig 2. Brightness comparison. Human peripheral blood was stained with CD4SBUV400 (MCA1267SBUV400) or CD4BUV395. SBUV400 is twice as bright as the competitor dye. BUV, Brilliant Ultraviolet; SBUV, StarBright UltraViolet; SI, staining index.

With minimum excitation and emission into other channels, and no special buffer required, SBUV400 can be easily added to existing panels to increase panel size or replace a fluorescent dye that has spillover into other channels. Examples of SBUV400 staining with other fluorescent dyes, including StarBright Dyes, can be seen in Figure 3.

Fig. 3. Performance of SBUV Dyes.


Fig. 3. Performance of SBUV Dyes. Human peripheral blood was stained with various SBUV400 labeled antibodies and counterstained with different StarBright Violet or PE labeled antibodies. Staining was performed in PBS containing 1% BSA.


StarBright UltraViolet 445 (SBUV445) Dye

SBUV445 is up to 20 times as bright as the nearest competitor dye (Figure 4) and allows clear separation of positive and negative populations, which is sometimes difficult when using competitor dyes with similar excitation and emission spectra, even with high antigen density.

Fig. 4. Brightness comparison. Human peripheral blood was stained with CDSBUV445 (MCA1267SBUV445) or CD4 DyLight 350. SBUV445 is over 20x as bright as the competitor dye. SBUV, StarBright UltraViolet; SI, stain index.


Fig. 4. Brightness comparison. Human peripheral blood was stained with CDSBUV445 (MCA1267SBUV445) or CD4 DyLight 350. SBUV445 is over 20x as bright as the competitor dye. SBUV, StarBright UltraViolet; SI, stain index.

SBUV445 is up to 20 times as bright as the nearest competitor dye (Figure 4) and allows clear separation of positive and negative populations, which is sometimes difficult when using dyes with similar excitation and emission spectra, even with high antigen density. SBUV445 is bright enough to detect rare populations, such as murine peripheral blood NK cells, upregulated markers upon activation such as CD25, and general immunophenotyping markers like CD27 (Figure 5). It is fixable, with no special buffer required when staining, and has high lot-to-lot reproducibility. SBUV445 can be easily added to existing panels, replacing dimmer dyes for enhanced resolution. As there are no other suitable dyes emitting at this wavelength from the 355 nm UV laser, this dye allows you to follow best practice, separating your markers across all lasers and filters. Furthermore, in spectral flow cytometry, as there are no competitor dyes, SBUV445 has a low similarity score, allowing you to increase panel size without increasing the overall complexity.

Fig. 5. Performance of SBUV445. Human peripheral blood was stained with various SBUV445 labeled antibodies and counterstained with a variety of StarBright Violet Dyes, StarBright Blue Dyes, or FITC labeled antibodies. Staining was performed in PBS containing 1% BSA with the addition of Fc block.


Fig. 5. Performance of SBUV445. Human peripheral blood was stained with various SBUV445 labeled antibodies and counterstained with a variety of StarBright Violet Dyes, StarBright Blue Dyes, or FITC labeled antibodies. Staining was performed in PBS containing 1% BSA with the addition of Fc block.


StarBright UltraViolet 510 (SBUV510) Dye

SBUV510 is a bright, 355 nm laser excitable dye that has all the benefits of StarBright Dyes and is suitable for use in both conventional and full spectrum flow cytometry (Figure 6).

Fig. 6. Brightness comparison.


Fig. 6. Brightness comparison. Human peripheral blood was stained with CDSBUV510 (MCA1267SBUV510) or CD4 BUV496. SBUV510 is twice as bright as the competitor dye. BUV, Brilliant Ultraviolet; SBUV, StarBright UltraViolet; SI, stain index.

SBUV510 is twice as bright as the nearest competitor dye (Figure 6) and is not a traditional tandem dye. Therefore, it has reduced signal in the channels expected from a donor dye. Fixable, with no special buffer required and lot-to-lot reproducibility compared to tandem dyes, SBUV510 can be easily added to existing panels and replace dimmer dyes for enhanced resolution.


StarBright UltraViolet 665 (SBUV665) Dye

SBUV665 Dye is a bright, 355 nm laser excitable dye that has all the benefits of StarBright Dyes, requires no special buffer and is suitable for use in conventional and full spectrum flow cytometry (Figure 7).

Fig 7. Brightness comparison


Fig. 7.
Brightness comparison. Human peripheral blood was stained with CD4SBUV665 (MCA1267SBUV665) or CD4BUV395. SBUV665 is twice as bright as the competitor dye. BUV, Brilliant Ultraviolet; SBUV, StarBright UltraViolet; SI, staining index.

Although SBUV665 exhibits reduced spillover, compared to BUV661, into Alexa 647 and Alexa 700, resolving weekly positive populations may still be an issue. You should follow panel building best practice and avoid marker combinations using dyes that can cause problems. This is demonstrated with CD127 (Figure 8), which has a broad expression range, and CD4. The CD127lo CD4hi cells are not distinguishable from CD127negCD4hi. When a marker such as CD3 is used, which does not have a CD3negCD4hi cluster, populations can be easily distinguished even when combined with Alexa 700. Spillover and spreading values are shown below (Table 2).

Fig. 8. Spillover considerations.


Fig. 8. Spillover considerations. Human peripheral blood was stained for CD4SBUV665 (MCA1267SBUV665), CD127 (HCA145A647), and CD3A700 (MCA463A700). The spillover and spreading is shown in the respective tables. 

Table 2. Spillover and spreading values for SBUV665.

Spillover:

Source

Target

 

SBUV665

A700

A647

SBUV665

1

31.7%

48.9%

A700

0%

1

1.9%

A647

0.5%

79%

1

Spreading:

Source

Target

 

SBUV665

A700

A647

SBUV665

0

1.01

1.72

A700

0.19

0

0.29

A647

0.11

2.46

0


StarBright UltraViolet 795 (SBUV795) Dye

SBUV795 is a bright, 355 nm laser excitable dye that has all the benefits of StarBright Dyes and is suitable for use in both conventional and full spectrum flow cytometry (Figure 9).

Fig. 9. Brightness comparison.


Fig. 9. Brightness comparison. Human peripheral blood was stained with CDSBUV795 (MCA1267SBUV795) or CD4 BUV805. SBUV795 is brighter than the competitor dye (over 2x). BUV, Brilliant Ultraviolet; SBUV, StarBright UltraViolet; SI, stain index.

SBUV795 is twice as bright as the nearest competitor dye (Figure 9) with a similar spillover profile. Fixable, with no special buffer required and lot-to-lot reproducibility not seen in tandem dyes, SBUV795 can be easily added to existing panels to expand the use of lasers and filters or replace dimmer dyes for enhanced resolution. Examples of SBUV795 staining in human and mouse samples with other fluorescent dyes, including multiplexing with other StarBright Dyes, can be seen in Figure 10.

Fig. 10. Performance of SBUV795


Fig. 10. Performance of SBUV795. Human peripheral blood was stained with various SBUV795 labeled antibodies and counterstained with a variety of StarBright UltraViolet, StarBright Violet, or Alexa labeled antibodies. Staining was performed in PBS containing 1% BSA with the addition of Fc block.



More Dyes Coming Soon

We will be expanding the SBUV range very soon with the launch of seven more dyes. With excellent brightness and spectral profiles, they give you extra flexibility in panel design and are ideal replacements to existing dyes excitable by the 355 nm laser. The exceptional performance of SBUV Dyes can be seen in Figure 11.

Fig. 11. Determination of co-stimulatory molecule expression on CD4+ T cells and labeling of CD19+ B cells.


Fig. 11. Determination of co-stimulatory molecule expression on CD4+ T cells and labeling of CD19+ B cells. Human peripheral blood was stained with CD19 SBUV605, CD3 SBUV400 (MCA463SBUV400), CD4 SBUV510, CD28 SBUV575, CD8 SBV710 (MCA1226SBV710), CD45RA SBV515 (MCA88SBV515), CD45RO SBV610 (MCA461SBV610), and CD27 SBV670 (MCA755SBV670) to identify A, B cells and T cells, B, T helper and T cytotoxic cells, and C, naïve or memory status of CD4+ T helper cells. Co-stimulatory molecule expression on (D) naïve and (E) memory cells was determined by the CD27 and CD28 expression. Staining was performed in PBS containing 1% BSA.

StarBright UltraViolet Antibodies

    DescriptionSpecificityTargetFormatHostIsotypeClone Applications Citations Product Type Code Validation Types

    Associated Reading

    For more information on how the StarBright Dye range can fit into your flow cytometry experiments, please take a look at our Starbright Dyes page.

    For more information on how these products can improve the effectiveness of your flow cytometry experiments, please take a look at our flow cytometry explained knowledge hub. Alternatively, feel free to explore the associated resource links below, for more in-depth information about each cited topic. If you are interested in learning the fundamentals of this application, please view our flow cytometry basics guide.