StarBright UltraViolet Dyes

Excitable by the 355 nm laser, StarBright UltraViolet (SBUV) Dyes are a perfect way to expand your flow cytometry panels, giving you more flexibility in your panel design while avoiding spillover and spreading. Available with maximal emissions ranging from 400 to 800 nm, eight additional dyes are coming soon with the same exceptional properties as the other StarBright Dyes and are conjugated to our validated antibodies. Suitable for use in conventional and full spectrum flow cytometry, these bright and stable dyes can be used in any buffer and have narrow excitation and emission profiles enabling you to build bigger and better panels.

SBUV Dye Range

StarBright Dye

Max Ex, nm

Max Em, nm

Brightness (15)

ZE5 Cell Analyzer Optimal Filter

Comparison Dye

Launch Date

Flier

StarBright UltraViolet 400 Dye

335

394

3

387/11

Brilliant Ultraviolet (BUV) 395

Available now

SBUV400 PDF

StarBright UltraViolet 445 Dye

347

440

3

447/60 or 460/22

Alexa Fluor 350

Coming soon

Coming soon

StarBright UltraViolet 510 Dye

340

513

3

509/25 or 525/50

BUV496

Coming soon

Coming soon

StarBright UltraViolet 575 Dye

340

569

4

577/15

BUV563

Coming soon

Coming soon

StarBright UltraViolet 605 Dye

340

609

4

615/24

BUV615

Coming soon

Coming soon

StarBright UltraViolet 665 Dye

340

669

4

670/30

BUV661

Coming soon

Coming soon

StarBright UltraViolet 740 Dye

344

743

4

747/33

BUV737

Coming soon

Coming soon

StarBright UltraViolet 795 Dye

340

792

2

780LP

BUV805

Coming soon

Coming soon


Fig. 1. Excitation and emission spectra of StarBright UltraViolet Dyes.


Fig. 1. Excitation and emission spectra of StarBright UltraViolet Dyes.


StarBright UltraViolet 400

StarBright UltraViolet 400 (SBUV400) Dye is a bright, 355 nm laser excitable dye that has all the benefits of StarBright Dyes and is suitable for use in conventional and full spectrum flow cytometry.

With minimum excitation and emission into other channels, and no special buffer required, SBUV400 is ideal for adding to existing panels to increase panel size or as a replacement for a fluorescent dye that has spillover into other channels. Examples of SBUV400 staining with other fluorescent dyes, including StarBright Dyes, can be seen in Figure 2.

Fig. 2. Performance of SBUV Dyes.


Fig. 2. Performance of SBUV Dyes. Human peripheral blood was stained with various SBUV400 labeled antibodies and counterstained with different StarBright Violet or PE labeled antibodies. Staining was performed in PBS containing 1% BSA.


More Dyes Coming Soon

We will be expanding the SBUV range very soon with the launch of seven more dyes. With excellent brightness and spectral profiles, they give you extra flexibility in panel design and are ideal replacements to existing dyes excitable by the 355 nm laser. The exceptional performance of SBUV Dyes can be seen in Figure 3.

Fig. 3. Determination of co-stimulatory molecule expression on CD4+ T cells and labeling of CD19+ B cells.


Fig. 3. Determination of co-stimulatory molecule expression on CD4+ T cells and labeling of CD19+ B cells. Human peripheral blood was stained with CD19 SBUV605, CD3 SBUV400 (MCA463SBUV400), CD4 SBUV510, CS28SBUV575, CD8SBV710 (MCA1226SBV710), CD45RASBV515 (MCA88SBV515), CD45ROSBV610 (MCA461SBV610), and CD27SBV670 (MCA755SBV670) to identify A, B cells and T cells, B, T helper and T cytotoxic cells, and C, naïve or memory status of CD4+ T helper cells. Co-stimulatory molecule expression on (D) naïve and (E) memory cells was determined by the CD27 and CD28 expression. Staining was performed in PBS containing 1% BSA.

StarBright UltraViolet 400 Antibodies

    DescriptionSpecificityTargetFormatHostIsotypeClone Applications Citations Product Type Code

    Associated Reading

    For more information on how the StarBright Dye range can fit into your flow cytometry experiments, please take a look at our Starbright Dyes page.

    For more information on how these products can improve the effectiveness of your flow cytometry experiments, please take a look at our flow cytometry explained knowledge hub. Alternatively, feel free to explore the associated resource links below, for more in-depth information about each cited topic. If you are interested in learning the fundamentals of this application, please view our flow cytometry basics guide.