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HuCAL® Recombinant Monoclonal Antibody Generation Service

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Reagents Supporting Antibodies and Proteins Incorporating SpyTag

The innovative SpyTag and SpyCatcher technology has been employed by the scientific community in many ways to address the challenges of detecting and controlling proteins.

We have added SpyTag to our recombinant Fab antibodies and produced a set of SpyCatchers to couple to the Fab, to make TrailBlazer Antibodies.

The SpyTag-SpyCatcher technology enables site-directed conjugation of antibodies to labels such as HRP and biotin, and the formation of bivalent Fabs and Ig-like constructs with human, mouse, and rabbit isotypes.

All SpyCatchers and their derivatives (Table 1) are compatible with our recombinant antibodies that incorporate a SpyTag2 at the C-terminus of the heavy chain (format Fab-F-Spy2-H). SpyCatchers can be coupled to recombinant Fab antibodies as part of our TrailBlazer Antibody Custom Service. SpyCatchers can also be purchased from our catalog for coupling by the user to an antibody or protein with a reactive SpyTag.

Table 1. SpyCatcher Reagents.

Product

Description

Availability

Monovalent Format

Custom Service

Catalog Number

SpyCatcher2

SpyCatcher2 protein

Yes

TZC001

SpyCatcher2-CYS

SpyCatcher2 with an engineered cysteine residue; use for site-specific chemical conjugation to a label of choice

Yes

TZC001CYS

SpyCatcher2:Biotin

SpyCatcher2 conjugated to biotin

Yes

Inquire

SpyCatcher2:HRP

SpyCatcher2 conjugated to HRP

Yes

Inquire

SpyCatcher2:PE

Spycatcher2 conjugated to RPE

Inquire

Inquire

SpyCatcher3

SpyCatcher3 protein

Yes

TZC025

SpyCatcher3-CYS

SpyCatcher3 with an engineered cysteine residue; use for site-specific conjugation to a label of choice

Yes

TZC025CYS

Bivalent Format

Custom Service

Catalog Number

BiSpyCatcher2

BiSpyCatcher2 protein

Yes

TZC002

BiSpyCatcher2-CYS

BiSpyCatcher2 with one engineered cysteine residue; use for site-specific conjugation to a label of choice

Yes

TZC002CYS

BiSpyCatcher2-CYS3

BiSpyCatcher2 with three engineered cysteine residues; use for site-specific conjugation to a label of choice

Yes

TZC002CYS3

BiSpyCatcher2:Biotin

BiSpyCatcher2 conjugated to biotin

Yes

Inquire

BiSpyCatcher2:HRP

BiSpyCatcher2 conjugated to HRP

Yes

Coming soon

BiSpyCatcher2:PE

BiSpyCatcher2 conjugated to RPE

Inquire

Inquire

Ig-like Format

Custom Service

Catalog Number

hIgG1-FcSpyCatcher3

SpyCatcher3 fused to the hinge region, CH2, and CH3 of human IgG1

Yes

TZC009

hIgG1-FcSpyCatcher3:Biotin

hIgG1-FcSpyCatcher3 conjugated to biotin

Yes

Inquire

hIgG1-FcSpyCatcher3:HRP

hIgG1-FcSpyCatcher3 conjugated to HRP

Yes

Inquire

hIgG2-FcSpyCatcher3

SpyCatcher3 fused to the hinge region, CH2, and CH3 of human IgG2

Yes

TZC016

hIgG3-FcSpyCatcher3

SpyCatcher3 fused to the hinge region, CH2, and CH3 of human IgG3

Yes

TZC017

hIgG4-FcSpyCatcher3

SpyCatcher3 fused to the hinge region, CH2, and CH3 of human IgG4

Yes

TZC018

hIgG4-Pro-FcSpyCatcher3

SpyCatcher3 fused to the hinge region, CH2, and CH3 of human IgG4-Pro (S228P)

Yes

TZC019

hIgA-FcSpyCatcher3

SpyCatcher3 fused to the hinge region, CH2, and CH3 of human IgA

Yes

TZC020

mIgG2a-FcSpyCatcher3

SpyCatcher3 fused to the hinge region, CH2, and CH3 of mouse IgG2a

Yes

TZC012

rbIgG-FcSpyCatcher3

SpyCatcher3 fused to the hinge region, CH2, and CH3 of rabbit IgG

Yes

TZC013

Table 2. Supporting Reagents.

Description

Clone

Catalog Number

HuCAL Fab-F-Spy2-H Negative Control Antibody1

AbD18705ad

TZA003

1 Monovalent Fab antibody with (F) DYKDDDDK-, (Spy2) SpyTag version 2, and (H) His-6-tags.



Protocols to Get You Started





Resources

Technical Guide to SpyCatcher Products

Technical Guide to SpyCatcher Products

An essential guide to SpyCatcher products, and how they can be used with SpyTagged antibodies and proteins, plus answers to frequently asked questions.
 

TrailBlazer Antibody Custom Services

TrailBlazer Antibody Custom Services

Get more details about SpyTag technology, how it works with our custom antibody generation service, and see data for different experimental applications.
 


References

  • Hentrich C et al. (2021). Periplasmic expression of SpyTagged antibody fragments enables rapid modular antibody assembly. Cell Chemical Biology 28, 1-12. bioRxiv preprint
  • Keeble A and Howarth M (2020). Power to the protein: Enhancing and combining activities using the Spy toolbox. Chem. Sci., 11, 7,281-7,291.
  • Zakeri B et al. (2012). Peptide Tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. PNAS 109(12), 690-697.