BMP-2 antibody

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Rabbit anti Human BMP-2:Biotin

Rabbit anti Human BMP-2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP960B E WB 50 µg
AHP960 E IF P* WB 50 µg
Rabbit anti Human BMP-2 antibody recognizes the disulphide-linked homodimeric cysteine knot protein known as human Bone Morphogenetic Protein 2 (BMP-2/BMP-2a), full-length 396 amino acids. BMP-2 is a member of the Transforming Growth Factor beta (TGF-B) superfamily and one of a growing number of osteogenic proteins shown to induce bone and cartilage formation and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis.

BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation.

The realization that BMP-2 was involved in the stimulation of bone formation emerged from research in spinal surgery, following the discovery that the healing of bones was directed by proteins contained within the bone matrix itself.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - liquid
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
N.B. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antisera to human BMP-2 were raised by repeated immunisations of rabbits with highly purified antigen.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human BMP-2
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P12643 Related reagents
Entrez Gene
BMP2 Related reagents
GO Terms
GO:0001649 osteoblast differentiation
GO:0001938 positive regulation of endothelial cell proliferation
GO:0003130 BMP signaling pathway involved in heart induction
GO:0005125 cytokine activity
GO:0005160 transforming growth factor beta receptor binding
GO:0005615 extracellular space
GO:0040007 growth
GO:0008083 growth factor activity
GO:0008285 negative regulation of cell proliferation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0010922 positive regulation of phosphatase activity
GO:0016362 activin receptor activity, type II
GO:0016563 transcription activator activity
GO:0016566 specific transcriptional repressor activity
GO:0019211 phosphatase activator activity
GO:0030501 positive regulation of bone mineralization
GO:0035051 cardiac cell differentiation
GO:0035630 bone mineralization involved in bone maturation
GO:0043065 positive regulation of apoptosis
GO:0045669 positive regulation of osteoblast differentiation
GO:0045786 negative regulation of cell cycle
GO:0046332 SMAD binding
GO:0046982 protein heterodimerization activity
GO:0051216 cartilage development
GO:0060317 cardiac epithelial to mesenchymal transition
GO:0060389 pathway-restricted SMAD protein phosphorylation
GO:0060395 SMAD protein signal transduction
GO:0061036 positive regulation of cartilage development
GO:0070700 BMP receptor binding
Regulatory
For research purposes only

Applications of BMP-2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 ug/ml 1.0 ug/ml
Western Blotting 0.1 ug/ml 0.2 ug/ml
ELISA 0.5 5.0ug/ml
Immunofluorescence
Immunohistology - Paraffin 1 0.25ug/ml
Western Blotting 0.1 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This biotinylated human BMP-2 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human BMP-2 antibody (AHP960) as the capture reagent.
ELISA
This purified human BMP-2antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human BMP-2 antibody (AHP960B) as the detection reagent.
Western Blotting
This antibody may be used in Western Blotting under either reducing or non-reducing conditions.
Western Blotting
This antibody may be used in Western Blotting under either reducing or non-reducing conditions.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for BMP-2 antibody

  1. Bessa, P.C. et al. (2008) Osteoinduction in human fat-derived stem cells by recombinant human bone morphogenetic protein-2 produced in Escherichia coli.
    Biotechnol Lett. 30 (1): 15-21.

Further Reading

  1. Kimura, N. et al. (2000) BMP2-induced apoptosis is mediated by activation of the TAK1-p38 kinase pathway that is negatively regulated by Smad6.
    J Biol Chem. 275 (23): 17647-52.
  2. Kirsch, T. et al. (2000) BMP-2 antagonists emerge from alterations in the low-affinity binding epitope for receptor BMPR-II.
    EMBO J. 19 (13): 3314-24.
  3. Keller, S. et al. (2004) Molecular recognition of BMP-2 and BMP receptor IA.
    Nat Struct Mol Biol. 11 (5): 481-8.
  4. Balemans, W. & VanHul, W. (2002) Extracellular regulation of BMP signaling in vertebrates: a cocktail of modulators.
    Dev Biol. 250 (2): 231-50.
  5. Sebald, W. et al. (2004) Molecular recognition in bone morphogenetic protein (BMP)/receptor interaction.
    Biol Chem. 385 (8): 697-710.