BMP-2 antibody

Rabbit anti Human BMP-2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
BMP-2

Product Code Applications Pack Size List Price Your Price Qty
AHP960
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Rabbit anti Human BMP-2 antibody recognizes the disulphide-linked homodimeric cysteine knot protein known as human Bone Morphogenetic Protein 2 (BMP-2/BMP-2a), full-length 396 amino acids. BMP-2 is a member of the Transforming Growth Factor beta (TGF-B) superfamily and one of a growing number of osteogenic proteins shown to induce bone and cartilage formation and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis.

BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation.

The realization that BMP-2 was involved in the stimulation of bone formation emerged from research in spinal surgery, following the discovery that the healing of bones was directed by proteins contained within the bone matrix itself.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human BMP-2 were raised by repeated immunisations of rabbits with highly purified antigen.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 5.0ug/ml
Immunofluorescence
Immunohistology - Paraffin 1 0.25ug/ml
Western Blotting 0.1 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This purified human BMP-2antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human BMP-2 antibody (AHP960B) as the detection reagent.
Western Blotting
This antibody may be used in Western Blotting under either reducing or non-reducing conditions.

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Description Sheep anti Rabbit IgG:FITC
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
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Description Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
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Description Sheep anti Rabbit IgG:RPE
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
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Description Goat anti Rabbit IgG (Fc):Biotin
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
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Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
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Description Goat anti Rabbit IgG (Fc):HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
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Description Antigen Retrieval Buffer, pH8.0
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
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Description TidyBlot Western Blot Detection Reagent:HRP

References for BMP-2 antibody

  1. Bessa, P.C. et al. (2008) Osteoinduction in human fat-derived stem cells by recombinant human bone morphogenetic protein-2 produced in Escherichia coli.
    Biotechnol Lett. 30 (1): 15-21.

Further Reading

  1. Kimura, N. et al. (2000) BMP2-induced apoptosis is mediated by activation of the TAK1-p38 kinase pathway that is negatively regulated by Smad6.
    J Biol Chem. 275 (23): 17647-52.
  2. Kirsch, T. et al. (2000) BMP-2 antagonists emerge from alterations in the low-affinity binding epitope for receptor BMPR-II.
    EMBO J. 19 (13): 3314-24.
  3. Keller, S. et al. (2004) Molecular recognition of BMP-2 and BMP receptor IA.
    Nat Struct Mol Biol. 11 (5): 481-8.
  4. Balemans, W. & VanHul, W. (2002) Extracellular regulation of BMP signaling in vertebrates: a cocktail of modulators.
    Dev Biol. 250 (2): 231-50.
  5. Sebald, W. et al. (2004) Molecular recognition in bone morphogenetic protein (BMP)/receptor interaction.
    Biol Chem. 385 (8): 697-710.

RRID
AB_609564
UniProt
P12643
Entrez Gene
BMP2
GO Terms
GO:0001649 osteoblast differentiation
GO:0001938 positive regulation of endothelial cell proliferation
GO:0003130 BMP signaling pathway involved in heart induction
GO:0005125 cytokine activity
GO:0005160 transforming growth factor beta receptor binding
GO:0005615 extracellular space
GO:0040007 growth
GO:0008083 growth factor activity
GO:0008285 negative regulation of cell proliferation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0010922 positive regulation of phosphatase activity
GO:0016362 activin receptor activity, type II
GO:0016563 transcription activator activity
GO:0016566 specific transcriptional repressor activity
GO:0019211 phosphatase activator activity
GO:0030501 positive regulation of bone mineralization
GO:0035051 cardiac cell differentiation
GO:0035630 bone mineralization involved in bone maturation
GO:0043065 positive regulation of apoptosis
GO:0045669 positive regulation of osteoblast differentiation
GO:0045786 negative regulation of cell cycle
GO:0046332 SMAD binding
GO:0046982 protein heterodimerization activity
GO:0051216 cartilage development
GO:0060317 cardiac epithelial to mesenchymal transition
GO:0060389 pathway-restricted SMAD protein phosphorylation
GO:0060395 SMAD protein signal transduction
GO:0061036 positive regulation of cartilage development
GO:0070700 BMP receptor binding
View more products with BMP specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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