IL-2 antibody
Rabbit anti Human Interleukin-2:Biotin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-2
Antibody Quality and Performance Guarantee
| Rabbit anti Human Interleukin-2 antibody recognizes human IL-2 (Interleukin-2), otherwise known as T-cell growth factor, a 133 amino acid glycoprotein and potent immunoregulatory cytokine produced by antigen or mitogen stimulated T cells. IL-2 is a multi-functional cytokine which signals through the IL-2 receptor complex (IL-2R), and is principally involved in the stimulation of the T cell proliferative response, promoting T cell clonal expansion and the expansion of T memory cells expressing low levels of high-affinity IL-2R. IL-2 also stimulates the growth/differentiation of B cells, monocytes, NK cells, macrophages and lymphokine activated killer cells. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antisera to human IL-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human IL-2
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstituiton.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.25 | 1.0ug/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This biotinylated human IL-2 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human IL-2 antibody (AHP1032) and recombinant human IL-2 (PHP042) as the standard.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Human Interleukin-2 | AHP1032 | E FN P * WB | 0.1 mg |
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| Description | Rabbit anti Human Interleukin-2 | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Recombinant Human Interleukin-2 | PHP042A | E FN WB | 10 µg | Log in | |||
| List Price | Your Price | ||||||
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| Description | Recombinant Human Interleukin-2 | ||||||
Further Reading
-
Fujita, T. et al. (1983) Structure of the human interleukin 2 gene.
Proc Natl Acad Sci U S A. 80 (24): 7437-41. -
Nelson, B.H. (2004) IL-2, regulatory T cells, and tolerance.
J Immunol. 172 (7): 3983-8.
- RRID
- AB_2125582
- UniProt
- P60568
- Entrez Gene
- IL2
- GO Terms
- GO:0002903 negative regulation of B cell apoptosis
- GO:0007155 cell adhesion
- GO:0005125 cytokine activity
- GO:0005134 interleukin-2 receptor binding
- GO:0005615 extracellular space
- GO:0006916 anti-apoptosis
- GO:0006955 immune response
- GO:0007267 cell-cell signaling
- GO:0008083 growth factor activity
- View More GO Terms
- GO:0019209 kinase activator activity
- GO:0030101 natural killer cell activation
- GO:0030217 T cell differentiation
- GO:0030307 positive regulation of cell growth
- GO:0030890 positive regulation of B cell proliferation
- GO:0032740 positive regulation of interleukin-17 production
- GO:0034105 positive regulation of tissue remodeling
- GO:0042104 positive regulation of activated T cell proliferation
- GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
- GO:0050729 positive regulation of inflammatory response
View more products with IL-2 specificity
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