IGF-I antibody

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Rabbit anti Human Insulin-Like Growth Factor I:Biotin

Rabbit anti Human Insulin-Like Growth Factor I

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1281B E WB 50 µg
AHP1281 E FN P* WB 0.1 mg
Rabbit anti Human insulin-likegGrowth factor I antibody recognizes human IGF-I (Insulin-like Growth Factor I), a 70 amino acid secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver. IGF-I exhibits both autocrine and paracrine properties and has a widespread effect on cells including those of the bone, cartilage, muscle, nerves, skin, liver and lungs, playing a key role in cell proliferation and growth, inhibition of apoptosis and linked with tumour cell growth.

IGF-I signals through binding to the type 1 insulin-like growth factor receptor (IGF-1R) and also to the insulin receptor (IR), resulting in the activation of the AKT signalling pathway. Production of IGF-I is stimulated by growth hormone (GH) and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signalling pathway.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation
Antisera to human IGF-I were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Immunogen
Recombinant human IGF-I
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.
Shelf Life
3 months from date of reconstitution.

Please see label for expiry date.

More Information

UniProt
P05019 Related reagents
Entrez Gene
IGF1 Related reagents
GO Terms
GO:0006260 DNA replication
GO:0002576 platelet degranulation
GO:0005158 insulin receptor binding
GO:0005159 insulin-like growth factor receptor binding
GO:0005179 hormone activity
GO:0006916 anti-apoptosis
GO:0006928 cellular component movement
GO:0007265 Ras protein signal transduction
GO:0007517 muscle organ development
GO:0008083 growth factor activity
GO:0009441 glycolate metabolic process
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
GO:0014834 satellite cell maintenance involved in skeletal muscle regeneration
GO:0014896 muscle hypertrophy
GO:0014904 myotube cell development
GO:0014911 positive regulation of smooth muscle cell migration
GO:0046326 positive regulation of glucose import
GO:0016942 insulin-like growth factor binding protein complex
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0034392 negative regulation of smooth muscle cell apoptosis
GO:0035630 bone mineralization involved in bone maturation
GO:0040014 regulation of multicellular organism growth
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0043568 positive regulation of insulin-like growth factor receptor signaling pathway
GO:0045445 myoblast differentiation
GO:0045669 positive regulation of osteoblast differentiation
GO:0045725 positive regulation of glycogen biosynthetic process
GO:0045740 positive regulation of DNA replication
GO:0045821 positive regulation of glycolysis
GO:0045840 positive regulation of mitosis
GO:0046579 positive regulation of Ras protein signal transduction
GO:0048146 positive regulation of fibroblast proliferation
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0050679 positive regulation of epithelial cell proliferation
GO:0051450 myoblast proliferation
Regulatory
For research purposes only

Applications of IGF-I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
ELISA 0.5 2.0ug/ml
Functional Assays 0.67 1.0ug/ml
Immunohistology - Paraffin 1 0.25ug/ml
Western Blotting 0.1 0.2ug/ml
  1. 1This antibody does not require antigen retrieval for use with formalin-fixed, paraffin-embedded sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This biotinylated human IGF-I antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human IGF-I antibody (AHP1281) as the capture reagent and recombinant human IGF-I (PHP177) as the standard.
ELISA
This purified human IGF-I antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human IGF-I antibody (AHP1281B) as the detection reagent and recombinant human IGF-I (PHP177) as the standard.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Singer, C.F. et al. (2004) Insulin-like growth factor (IGF)-I and IGF-II serum concentrations in patients with benign and malignant breast lesions: free IGF-II is correlated with breast cancer size.
    Clin Cancer Res. 10 (12 Pt 1): 4003-9.