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IGF-I antibody

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Anti Human Insulin-Like Growth Factor I Antibody thumbnail image 1
Anti Human Insulin-Like Growth Factor I Antibody gallery image 1
Western blot analysis of recombinant human IGF-1 (PHP177; reduced) probed with Rabbit anti Human insulin-like Growth factor I antibody (AHP1281) followed by the detection with HRP conjugated Goat anti Rabbit IgG (STAR208P) at 1/10,000. Different dilutions of AHP1281 are shown on lane 2 (0.2 ug/ml) and lane 4 (0.1 ug/ml). STAR208P only at a 1/10,000 dilution was included as a secondary only control (lane 6).

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Rabbit anti Human Insulin-Like Growth Factor I

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IGF-I

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Product Code Applications Pack Size List Price Your Price Qty
AHP1281
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN P * WB 0.1 mg loader
List Price Your Price
loader

Rabbit anti Human insulin-like Growth factor I antibody recognizes human IGF-I (Insulin-like Growth Factor I), a 70 amino acid secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver. IGF-I exhibits both autocrine and paracrine properties and has a widespread effect on cells including those of the bone, cartilage, muscle, nerves, skin, liver and lungs, playing a key role in cell proliferation and growth, inhibition of apoptosis and linked with tumour cell growth.

IGF-I signals through binding to the type 1 insulin-like growth factor receptor (IGF-1R) and also to the insulin receptor (IR), resulting in the activation of the AKT signalling pathway. Production of IGF-I is stimulated by growth hormone (GH) and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signalling pathway.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation
Antisera to human IGF-I were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 0.67 1.0ug/ml
Immunohistology - Paraffin 1 0.25ug/ml
Western Blotting 0.1 0.2ug/ml
  1. 1This antibody does not require antigen retrieval for use with formalin-fixed, paraffin-embedded sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This purified human IGF-I antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human IGF-I antibody (AHP1281B) as the detection reagent and recombinant human IGF-I (PHP177) as the standard.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):Biotin
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
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loader
Description Goat anti Rabbit IgG (Fc):HRP
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:FITC
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB * 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

Further Reading

  1. Singer, C.F. et al. (2004) Insulin-like growth factor (IGF)-I and IGF-II serum concentrations in patients with benign and malignant breast lesions: free IGF-II is correlated with breast cancer size.
    Clin Cancer Res. 10 (12 Pt 1): 4003-9.

Synonyms
Insulin-Like Growth Factor I
RRID
AB_2122111
UniProt
P05019
Entrez Gene
IGF1
GO Terms
GO:0006260 DNA replication
GO:0002576 platelet degranulation
GO:0005158 insulin receptor binding
GO:0005159 insulin-like growth factor receptor binding
GO:0005179 hormone activity
GO:0006916 anti-apoptosis
GO:0006928 cellular component movement
GO:0007265 Ras protein signal transduction
GO:0007517 muscle organ development
GO:0008083 growth factor activity
GO:0009441 glycolate metabolic process
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
GO:0014834 satellite cell maintenance involved in skeletal muscle regeneration
GO:0014896 muscle hypertrophy
GO:0014904 myotube cell development
GO:0014911 positive regulation of smooth muscle cell migration
GO:0046326 positive regulation of glucose import
GO:0016942 insulin-like growth factor binding protein complex
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0034392 negative regulation of smooth muscle cell apoptosis
GO:0035630 bone mineralization involved in bone maturation
GO:0040014 regulation of multicellular organism growth
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0043568 positive regulation of insulin-like growth factor receptor signaling pathway
GO:0045445 myoblast differentiation
GO:0045669 positive regulation of osteoblast differentiation
GO:0045725 positive regulation of glycogen biosynthetic process
GO:0045740 positive regulation of DNA replication
GO:0045821 positive regulation of glycolysis
GO:0045840 positive regulation of mitosis
GO:0046579 positive regulation of Ras protein signal transduction
GO:0048146 positive regulation of fibroblast proliferation
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0050679 positive regulation of epithelial cell proliferation
GO:0051450 myoblast proliferation

AHP1281

146050 150477 166877

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