IP-10 antibody
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Rabbit anti Mouse IP-10
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IP-10
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| Rabbit anti Mouse IP-10 antibody recognizes interferon gamma inducible protein (IP-10), also known as CXCL10. IP-10 is expressed in a variety of tissues including liver, gut, spleen, kidney and thymus, in response to interferon gamma. IP-10 acts as a chemoattractant for monocytes, NK cells and certain T-cells. |
- Target Species
- Mouse
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to mouse IP-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant mouse IP-10.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 6 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5ug/ml | |
| Functional Assays | |
0.5ug/ml | 0.9ug/ml |
| Immunohistology - Frozen | |
||
| Western Blotting | |
0.1ug/ml | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
- ELISA
- Purified rabbit anti mouse IP-10 may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with our biotinylated anti mouse IP-10 antibody (AAM39B) as a detection reagent.
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References for IP-10 antibody
-
Zhou, J. et al. (2010) CXCR3-dependent accumulation and activation of perivascular macrophages is necessary for homeostatic arterial remodeling to hemodynamic stresses.
J Exp Med. 207 (9): 1951-66.
- Synonyms
- CXCL10
- RRID
- AB_2230175
- UniProt
- P17515
- Entrez Gene
- Cxcl10
- GO Terms
- GO:0005615 extracellular space
- GO:0006935 chemotaxis
- GO:0006954 inflammatory response
- GO:0008009 chemokine activity
- GO:0009897 external side of plasma membrane
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