IP-10 antibody

Rabbit anti Mouse IP-10

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IP-10

Product Code Applications Pack Size List Price Your Price Qty
AAM39
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E FN WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti Mouse IP-10 antibody recognizes interferon gamma inducible protein (IP-10), also known as CXCL10. IP-10 is expressed in a variety of tissues including liver, gut, spleen, kidney and thymus, in response to interferon gamma.

IP-10 acts as a chemoattractant for monocytes, NK cells and certain T-cells.

Target Species
Mouse
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water

Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to mouse IP-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant mouse IP-10.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
6 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Functional Assays 0.5ug/ml 0.9ug/ml
Immunohistology - Frozen
Western Blotting 0.1ug/ml 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
ELISA
Purified rabbit anti mouse IP-10 may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with our biotinylated anti mouse IP-10 antibody (AAM39B) as a detection reagent.

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References for IP-10 antibody

  1. Zhou, J. et al. (2010) CXCR3-dependent accumulation and activation of perivascular macrophages is necessary for homeostatic arterial remodeling to hemodynamic stresses.
    J Exp Med. 207 (9): 1951-66.

Immunohistology - Frozen

Synonyms
CXCL10
RRID
AB_2230175
UniProt
P17515
Entrez Gene
Cxcl10
GO Terms
GO:0005615 extracellular space
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0008009 chemokine activity
GO:0009897 external side of plasma membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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