Coming soon — Excitable by the 640 nm laser, StarBright Red (SBR) Dyes, are the perfect dyes to expand your capabilities in both conventional and full spectrum flow cytometry.
Designed to be bright, used in any buffer and with outstanding stability, StarBright Red Dyes should be your first choice when building panels using the 640 nm laser.
StarBright Dye |
Max Ex, nm |
Max Em, nm |
Brightness (1-5) |
ZE5 Cell Analyzer Optimal Filter |
Comparison Dyes |
Launch Date |
Flier |
---|---|---|---|---|---|---|---|
StarBright Red 670 |
653 |
666 |
|
670/30 |
A647, APC |
Coming soon |
|
StarBright Red 715 |
638 |
712 |
|
720/50 |
A700,APC-R700 |
Coming soon |
|
StarBright Red 775 |
653 |
778 |
|
775/50 |
APC-Cy7, APC-H7, APC-Fire750 |
Coming soon |
|
StarBright Red 815 |
654 |
811 |
|
800LP |
APC-Fire810 |
Coming soon |
|
Fig. 1. Excitation and emission spectra of StarBright Red Dyes.
StarBright Red Dyes are perfect replacements for APC and APC tandems in your flow cytometry experiments. They are bright, with unique spectra, not photosensitive, and can be fixed in both formaldehyde and alcohol-based fixatives with minimal changes in performance and spectra, making these dyes an excellent choice for both conventional and full spectrum flow cytometry.
StarBright Red Dyes can be used either with/without a special buffer depending on the requirements of the fluorophores that the StarBright Red Dyes are multiplexed with, offering you flexibility when building panels.
Figure 2 shows how they can be used to identify cell subsets in human peripheral blood.
Fig. 2. Immunophenotyping panels including StarBright Red Dye CD4 conjugated antibodies. Red blood cell lysed human peripheral blood was stained with A. CD19SBUV665 (MCA1940SBUV665), CD3PE (MCA463PE), CD8SBV790 (MCA1226SBV790), CD45RASBV670 (MCA88SBV670), CD45ROSBUV795 (MCA461SBUV795) and CD4SBR670, or B. CD14PE-A750 (MCA1568P750), CD57FITC (MCA1305) and CD4 SBR775. Cells were gated on live, single cells (not shown) and analyzed on the ZE5 Cell Analyzer.
For more information on how these products can improve the effectiveness of your flow cytometry experiments, please take a look at our StarBright Dyes page.
Useful information about fluorescent dyes and immunophenotyping can be found in our flow cytometry explained knowledge hub. Alternatively, feel free to explore the associated resource links below, for more in-depth information about each cited topic. If you are interested in learning the fundamentals of this application, please view our flow cytometry basics guide.