StarBright Red Dyes

Excitable by the 640 nm laser, StarBright™ Red (SBR) Dyes, are the perfect dyes to expand your capabilities in both conventional and full spectrum flow cytometry.

Designed to be bright, used in any buffer and with outstanding stability, StarBright Red Dyes should be your first choice when building panels using the 640 nm laser.

StarBright Red Dye Range

StarBright Dye	Max Ex, nm	Max Em, nm	ZE5 Cell Analyzer Optimal Filter	Comparison Dyes	Launch Date
StarBright Red 670	653	666	670/30	A647, APC	Available now
StarBright Red 715	638	712	720/50	A700,APC-R700	Coming soon
StarBright Red 775	653	778	775/50	APC-Cy7, APC-H7, APC-Fire750	Available now
StarBright Red 815	654	811	800LP	APC-Fire810	Coming soon

Fig. 1. Excitation and emission spectra of StarBright Red Dyes.

StarBright Red Dyes are perfect replacements for APC and APC tandems in your flow cytometry experiments. They are bright, with unique spectra, not photosensitive, and can be fixed in both formaldehyde and alcohol-based fixatives with minimal changes in performance and spectra, making these dyes an excellent choice for both conventional and full spectrum flow cytometry.

StarBright Red Dyes can be used either with/without a special buffer depending on the requirements of the fluorophores that the StarBright Red Dyes are multiplexed with, offering you flexibility when building panels.

StarBright Red 670 (SBR670) Dye

Fig. 2. Brightness comparison. Human peripheral blood was stained with CD4 SBR670 (MCA1267SBR670) (red), or CD4 A647 (MCA1267A647) (blue) and analyzed on the ZE5 Cell Analyzer detected using the 670/30 filter. All antibodies were titrated prior to use to determine the optimal concentration.

Fig. 2. Brightness comparison. Human peripheral blood was stained with CD4 SBR670 (MCA1267SBR670) (red), or CD4 A647 (MCA1267A647) (blue) and analyzed on the ZE5 Cell Analyzer detected using the 670/30 filter. All antibodies were titrated prior to use to determine the optimal concentration.

SBR670 is the first of our new StarBright Red Dye series. It is a bright 640 nm laser excitable dye emitting at 666 nm with a narrow excitation and emission profile.

SBR670 has been found to be brighter than Alexa Fluor 647 (A647) (Figure 2) and Allophycocyanin (APC) (not shown). It can be used with any common staining buffer and can be fixed in PFA or alcohol based buffers with minimal loss of signal.

As it has a unique spectra, StarBright Red 670 Dye can be used with A647 and APC in full spectrum flow cytometry multicolor panels.

View All Our StarBright Red 670 Antibodies

Figure 3 shows how SBR Dyes can be used to identify cell subsets in human peripheral blood.

Fig. 3. Immunophenotyping panels including StarBright Red Dye CD4 conjugated antibodies. Red blood cell lysed human peripheral blood was stained with A. CD19SBUV665 (MCA1940SBUV665), CD3PE (MCA463PE), CD8SBV790 (MCA1226SBV790), CD45RASBV670 (MCA88SBV670), CD45ROSBUV795 (MCA461SBUV795), and CD4SBR670 (MCA1267SBR670), or B. CD14PE-A750 (MCA1568P750), CD57FITC (MCA1305), and CD4 SBR775. Cells were gated on live, single cells (not shown) and analyzed on the ZE5 Cell Analyzer.

StarBright Red Antibodies

Description	Specificity	Target	Format	Host	Isotype	Clone	Applications	Citations	Product Type	Code	Validation Types

Associated Reading

For more information on how these products can improve the effectiveness of your flow cytometry experiments, please take a look at our StarBright Dyes page.

Useful information about fluorescent dyes and immunophenotyping can be found in our flow cytometry explained knowledge hub. Alternatively, feel free to explore the associated resource links below, for more in-depth information about each cited topic. If you are interested in learning the fundamentals of this application, please view our flow cytometry basics guide.

Resources for Flow Cytometry